Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V5F97_RS10210 Genome accession   NZ_CP145057
Coordinates   1953678..1954721 (-) Length   347 a.a.
NCBI ID   WP_353114497.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain WHO_alpha_2024     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1948678..1959721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F97_RS10195 (V5F97_10235) - 1949257..1949541 (+) 285 WP_003692093.1 GIY-YIG nuclease family protein -
  V5F97_RS10200 (V5F97_10240) yccS 1950021..1952171 (-) 2151 WP_003697785.1 YccS family putative transporter -
  V5F97_RS10205 (V5F97_10245) pilU 1952288..1953514 (-) 1227 WP_003697786.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V5F97_RS10210 (V5F97_10250) pilT 1953678..1954721 (-) 1044 WP_353114497.1 type IV pilus twitching motility protein PilT Machinery gene
  V5F97_RS10215 (V5F97_10255) - 1954841..1955527 (+) 687 WP_003690201.1 YggS family pyridoxal phosphate-dependent enzyme -
  V5F97_RS10220 (V5F97_10260) - 1955527..1955934 (+) 408 WP_003701818.1 hypothetical protein -
  V5F97_RS10225 (V5F97_10265) proC 1955983..1956774 (+) 792 WP_003692105.1 pyrroline-5-carboxylate reductase -
  V5F97_RS10230 (V5F97_10270) dksA 1956924..1957340 (+) 417 WP_003692108.1 RNA polymerase-binding protein DksA -
  V5F97_RS10235 (V5F97_10275) - 1957355..1957513 (+) 159 WP_172761082.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37910.29 Da        Isoelectric Point: 6.8962

>NTDB_id=849673 V5F97_RS10210 WP_353114497.1 1953678..1954721(-) (pilT) [Neisseria gonorrhoeae strain WHO_alpha_2024]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLGFANALSSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEAARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=849673 V5F97_RS10210 WP_353114497.1 1953678..1954721(-) (pilT) [Neisseria gonorrhoeae strain WHO_alpha_2024]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGACATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGCCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACACCCTCGGCTTCGCCAA
CGCGCTGAGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGAGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTCCATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACGATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGCCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

99.712

100

0.997
  pilT Neisseria meningitidis 8013

98.847

100

0.988
  pilT Acinetobacter baumannii D1279779

68.116

99.424

0.677
  pilT Acinetobacter baumannii strain A118

68.116

99.424

0.677
  pilT Acinetobacter nosocomialis M2

68.116

99.424

0.677
  pilT Acinetobacter baylyi ADP1

67.536

99.424

0.671
  pilT Pseudomonas stutzeri DSM 10701

67.246

99.424

0.669
  pilT Pseudomonas aeruginosa PAK

66.667

99.424

0.663
  pilT Legionella pneumophila strain Lp02

64.223

98.271

0.631
  pilT Legionella pneumophila strain ERS1305867

64.223

98.271

0.631
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.939

95.101

0.608
  pilT Vibrio cholerae strain A1552

63.939

95.101

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.097

97.695

0.519
  pilU Pseudomonas stutzeri DSM 10701

40

99.424

0.398
  pilU Acinetobacter baylyi ADP1

38.15

99.712

0.38
  pilU Vibrio cholerae strain A1552

38.04

100

0.38