Detailed information NTG0849672

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V5F97_RS10205
• Genome accession: NZ_CP145057
• Coordinates: 1952288..1953514 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain WHO_alpha_2024
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1947288..1958514

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5F97_RS10195 (V5F97_10235)	-	1949257..1949541 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
V5F97_RS10200 (V5F97_10240)	yccS	1950021..1952171 (-)	2151	WP_003697785.1	YccS family putative transporter	-
V5F97_RS10205 (V5F97_10245)	pilU	1952288..1953514 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V5F97_RS10210 (V5F97_10250)	pilT	1953678..1954721 (-)	1044	WP_353114497.1	type IV pilus twitching motility protein PilT	Machinery gene
V5F97_RS10215 (V5F97_10255)	-	1954841..1955527 (+)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
V5F97_RS10220 (V5F97_10260)	-	1955527..1955934 (+)	408	WP_003701818.1	hypothetical protein	-
V5F97_RS10225 (V5F97_10265)	proC	1955983..1956774 (+)	792	WP_003692105.1	pyrroline-5-carboxylate reductase	-
V5F97_RS10230 (V5F97_10270)	dksA	1956924..1957340 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
V5F97_RS10235 (V5F97_10275)	-	1957355..1957513 (+)	159	WP_172761082.1	hypothetical protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=849672 V5F97_RS10205 WP_003697786.1 1952288..1953514(-) (pilU) [Neisseria gonorrhoeae strain WHO_alpha_2024]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=849672 V5F97_RS10205 WP_003697786.1 1952288..1953514(-) (pilU) [Neisseria gonorrhoeae strain WHO_alpha_2024]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387