Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JUH08_RS01950 Genome accession   NZ_AP024354
Coordinates   426524..427558 (+) Length   344 a.a.
NCBI ID   WP_021699006.1    Uniprot ID   U2ZIR1
Organism   Aquipseudomonas alcaligenes strain KAM426     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421524..432558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JUH08_RS01925 (KAM426_03880) - 421891..423879 (-) 1989 WP_061903058.1 dynamin-like GTPase family protein -
  JUH08_RS01930 (KAM426_03890) - 424026..424322 (-) 297 WP_061903059.1 DUF167 domain-containing protein -
  JUH08_RS01935 (KAM426_03900) - 424324..424917 (-) 594 WP_203790058.1 YggT family protein -
  JUH08_RS01940 (KAM426_03910) proC 424927..425745 (-) 819 WP_142135900.1 pyrroline-5-carboxylate reductase -
  JUH08_RS01945 (KAM426_03920) - 425759..426451 (-) 693 WP_203790060.1 YggS family pyridoxal phosphate-dependent enzyme -
  JUH08_RS01950 (KAM426_03930) pilT 426524..427558 (+) 1035 WP_021699006.1 type IV pilus twitching motility protein PilT Machinery gene
  JUH08_RS01955 (KAM426_03940) pilU 427616..428761 (+) 1146 WP_061903063.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JUH08_RS01960 (KAM426_03950) - 428715..429599 (-) 885 WP_203790062.1 TIGR03915 family putative DNA repair protein -
  JUH08_RS01965 (KAM426_03960) - 429599..430810 (-) 1212 WP_203790064.1 putative DNA modification/repair radical SAM protein -
  JUH08_RS01970 (KAM426_03970) - 430960..431358 (+) 399 WP_061903065.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38009.69 Da        Isoelectric Point: 6.8889

>NTDB_id=84925 JUH08_RS01950 WP_021699006.1 426524..427558(+) (pilT) [Aquipseudomonas alcaligenes strain KAM426]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDACL
KNLLSKGLVSRESAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=84925 JUH08_RS01950 WP_021699006.1 426524..427558(+) (pilT) [Aquipseudomonas alcaligenes strain KAM426]
ATGGATATTACCGAGCTGCTCGCCTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTCTCGGCGGGCCTGCCGCCGAT
GATTCGCGTCGACGGTGACGTACGACGGATCAACCTGCCGCCGATGGACCACAAACAGGTCCACGCGTTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCTGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTCCTGAGCATGGA
AGACCTCGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTGAACAACAACAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATTCTGGTCGGTGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAGGAGAAATCCATGGTCCGTTCCATGCTGTCGGAGTCGCTGCAGGCGGTGATTTCGCAGAC
CCTGCTGAAGAAGGTCGGCGGCGGTCGGGTGGCGGCCCACGAGATCATGATCGGCACTCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGTTCGCTGGGCATGCAGACCCTCGACGCCTGCCTG
AAGAACCTGCTGAGCAAGGGCCTGGTCAGTCGCGAGTCCGCGCGCGAGAAGGCCAAGACGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2ZIR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.64

100

0.956
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter nosocomialis M2

84.593

100

0.846
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Vibrio cholerae strain A1552

39.13

100

0.392

Multiple sequence alignment