Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QR722_RS15155 Genome accession   NZ_CP128185
Coordinates   3475797..3476840 (-) Length   347 a.a.
NCBI ID   WP_286283767.1    Uniprot ID   -
Organism   Aliiglaciecola sp. LCG003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3470797..3481840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR722_RS15125 (QR722_15125) cysK 3471240..3472220 (+) 981 WP_286283761.1 cysteine synthase A -
  QR722_RS15130 (QR722_15130) gshB 3472370..3473320 (+) 951 WP_286283762.1 glutathione synthase -
  QR722_RS15135 (QR722_15135) - 3473322..3473879 (+) 558 WP_286283763.1 YqgE/AlgH family protein -
  QR722_RS15140 (QR722_15140) ruvX 3473931..3474350 (+) 420 WP_286283764.1 Holliday junction resolvase RuvX -
  QR722_RS15145 (QR722_15145) - 3474337..3474672 (-) 336 WP_286283765.1 DUF2007 domain-containing protein -
  QR722_RS15150 (QR722_15150) pilU 3474675..3475787 (-) 1113 WP_286283766.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QR722_RS15155 (QR722_15155) pilT 3475797..3476840 (-) 1044 WP_286283767.1 type IV pilus twitching motility protein PilT Machinery gene
  QR722_RS15160 (QR722_15160) - 3476871..3477554 (+) 684 WP_286283768.1 YggS family pyridoxal phosphate-dependent enzyme -
  QR722_RS15165 (QR722_15165) proC 3477573..3478394 (+) 822 WP_286283769.1 pyrroline-5-carboxylate reductase -
  QR722_RS15170 (QR722_15170) - 3478410..3478940 (+) 531 WP_286283770.1 YggT family protein -
  QR722_RS15175 (QR722_15175) - 3478972..3479421 (+) 450 WP_286283771.1 DUF4426 domain-containing protein -
  QR722_RS15180 (QR722_15180) - 3479581..3480864 (+) 1284 WP_286283772.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38494.24 Da        Isoelectric Point: 6.7806

>NTDB_id=846562 QR722_RS15155 WP_286283767.1 3475797..3476840(-) (pilT) [Aliiglaciecola sp. LCG003]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRRLNVPELDHKQVHGLVYEIMNDRQRKEYEENLEVDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPAIFKDIINQPTGIVLVTGATGSGKSTTLAAMVDHINTYKREHILTIED
PIEFVHQNKLCVLNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMIGIPAIRNLIREDKVPQMYSVIQTGQSHGMQTMDQCL
QKLVAQGVISAQDAASKSIDKQPKIGF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=846562 QR722_RS15155 WP_286283767.1 3475797..3476840(-) (pilT) [Aliiglaciecola sp. LCG003]
GTGGATATTACCGAACTTTTAGCATTCAGTGTGAAGAATAATGCCTCTGATTTACATCTTTCAGCTGGCTTACCGCCGAT
TATTCGAGTCGATGGCGAAATGCGTCGCCTTAATGTGCCTGAGCTAGACCACAAACAAGTACATGGGCTGGTCTACGAAA
TTATGAATGATAGGCAGCGCAAGGAATATGAAGAAAACCTTGAAGTGGATTTTTCGTTTGAAGTGAAAGATCTATCTCGC
TTCCGGGTGAATGCATTCGTGCAAAACCGTGGCGCTGCAGCGGTATTAAGAACGATCCCGAGCAAGGTACTAACCTTGGA
CGATTTAGGTGCACCGGCTATCTTTAAAGATATCATCAACCAACCAACGGGTATTGTGCTGGTCACAGGCGCAACAGGCT
CAGGGAAAAGTACTACGCTGGCAGCTATGGTCGATCACATTAATACCTACAAGCGCGAACATATTCTGACCATTGAAGAC
CCGATTGAATTCGTCCATCAAAATAAATTGTGCGTGTTGAATCAACGGGAAGTGCATCGGGATACCCATAGCTTCAATAA
TGCCTTGCGCTCTGCGTTGCGGGAAGACCCTGATGTTATTCTGGTGGGTGAATTGCGAGACCTTGAGACAATTCGCTTAG
CCATTTCAGCGGCAGAAACCGGACACCTGGTGTTTGGAACCTTGCATACCAATTCCGCCCCCAAAACCATAGACCGGATT
ATTGATGTGTTCCCCGCAGAAGAAAAGTCGATGGTGCGTTCAATGTTATCCGAGTCGTTACGGGCTGTTATCTCTCAAAC
TTTATTGAAGAAAGTCGGTGGGGGGCGTGTGGCTGCTCATGAAATCATGATTGGCATTCCGGCGATTCGAAACTTGATCC
GTGAGGACAAGGTGCCGCAAATGTATTCAGTGATCCAGACCGGTCAAAGTCATGGTATGCAAACCATGGATCAATGTCTG
CAAAAACTCGTCGCTCAAGGGGTGATCAGTGCCCAAGATGCAGCGTCCAAATCCATTGATAAGCAACCCAAGATTGGTTT
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.326

99.135

0.767
  pilT Acinetobacter baumannii strain A118

77.326

99.135

0.767
  pilT Acinetobacter nosocomialis M2

77.035

99.135

0.764
  pilT Pseudomonas stutzeri DSM 10701

75

99.135

0.744
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

74.556

97.406

0.726
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.118

0.7
  pilT Vibrio cholerae strain A1552

72.107

97.118

0.7
  pilT Legionella pneumophila strain Lp02

71.261

98.271

0.7
  pilT Legionella pneumophila strain ERS1305867

71.261

98.271

0.7
  pilT Neisseria gonorrhoeae MS11

65.517

100

0.657
  pilT Neisseria meningitidis 8013

67.463

96.542

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.478

98.847

0.519
  pilU Pseudomonas stutzeri DSM 10701

42.773

97.695

0.418
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

41.369

96.83

0.401