Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QR722_RS15150 Genome accession   NZ_CP128185
Coordinates   3474675..3475787 (-) Length   370 a.a.
NCBI ID   WP_286283766.1    Uniprot ID   -
Organism   Aliiglaciecola sp. LCG003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3469675..3480787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR722_RS15120 (QR722_15120) - 3470661..3471113 (-) 453 WP_286283760.1 Lrp/AsnC family transcriptional regulator -
  QR722_RS15125 (QR722_15125) cysK 3471240..3472220 (+) 981 WP_286283761.1 cysteine synthase A -
  QR722_RS15130 (QR722_15130) gshB 3472370..3473320 (+) 951 WP_286283762.1 glutathione synthase -
  QR722_RS15135 (QR722_15135) - 3473322..3473879 (+) 558 WP_286283763.1 YqgE/AlgH family protein -
  QR722_RS15140 (QR722_15140) ruvX 3473931..3474350 (+) 420 WP_286283764.1 Holliday junction resolvase RuvX -
  QR722_RS15145 (QR722_15145) - 3474337..3474672 (-) 336 WP_286283765.1 DUF2007 domain-containing protein -
  QR722_RS15150 (QR722_15150) pilU 3474675..3475787 (-) 1113 WP_286283766.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QR722_RS15155 (QR722_15155) pilT 3475797..3476840 (-) 1044 WP_286283767.1 type IV pilus twitching motility protein PilT Machinery gene
  QR722_RS15160 (QR722_15160) - 3476871..3477554 (+) 684 WP_286283768.1 YggS family pyridoxal phosphate-dependent enzyme -
  QR722_RS15165 (QR722_15165) proC 3477573..3478394 (+) 822 WP_286283769.1 pyrroline-5-carboxylate reductase -
  QR722_RS15170 (QR722_15170) - 3478410..3478940 (+) 531 WP_286283770.1 YggT family protein -
  QR722_RS15175 (QR722_15175) - 3478972..3479421 (+) 450 WP_286283771.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40953.09 Da        Isoelectric Point: 5.5886

>NTDB_id=846561 QR722_RS15150 WP_286283766.1 3474675..3475787(-) (pilU) [Aliiglaciecola sp. LCG003]
MDLNPFLRKMAEQGASDLFVTVGFPISAKINGQMTPITDECLTEEAALALVQNVMSDKQRDEFETSKECNFAIARDGIGR
FRCSAFWQRDMAGMVVRRIVTQIPKADDLGLPEILKDVIMAKRGLLLFVGATGTGKSTSLAALMGHRNHHSRGHILTIED
PIEFVHEHAGCVVTQREVGIDTRSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPQEQHAKLRFDLSLNLRAIVAQQLVPTIDGKGRVAAIEILLNSPLVTDLIQRNEIGSLKEAMKKGKEMGMQSFDMA
LYELYKEQRISLEQAIHHADSPNDLRLMIKLDTEEGTGLGSLSNVSIDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=846561 QR722_RS15150 WP_286283766.1 3474675..3475787(-) (pilU) [Aliiglaciecola sp. LCG003]
ATGGACTTAAATCCGTTTTTACGAAAAATGGCCGAGCAGGGGGCTTCGGATCTATTTGTCACTGTGGGCTTTCCTATTAG
CGCTAAGATAAATGGTCAAATGACGCCGATTACCGATGAATGCTTAACCGAAGAGGCAGCATTGGCGTTGGTGCAAAATG
TAATGAGTGATAAACAACGGGACGAATTCGAGACTAGCAAAGAGTGTAACTTTGCTATTGCCAGAGATGGCATAGGGCGA
TTTCGTTGCAGTGCATTTTGGCAGCGTGATATGGCGGGCATGGTTGTGCGCCGGATCGTGACTCAGATTCCCAAAGCCGA
TGACTTAGGTCTGCCGGAGATCCTAAAAGATGTGATAATGGCAAAGCGTGGCTTACTGTTATTCGTTGGTGCTACCGGAA
CAGGTAAGTCAACCTCTTTAGCAGCCCTTATGGGACATCGTAATCACCATTCCCGTGGGCATATTTTAACTATCGAGGAC
CCGATTGAATTTGTGCATGAACATGCCGGATGTGTTGTCACTCAGCGGGAAGTGGGTATTGATACTCGCTCATTCGATGA
TGCTTTAAAAAGTTCTCTGCGCCAAGCGCCTGATGTAATTCTCATCGGTGAAATTCGGTCAATGGAAACCATGGAATATG
CTATGTCGTTCGCCGATACAGGCCATTTGTGTGTGGCGACATTACATGCCAATAATGCTAACCAAGCAATCGAACGTATC
ATGCACTTAGCCCCGCAGGAGCAGCACGCGAAGTTGCGCTTTGATTTGAGTTTAAATTTGAGGGCGATTGTCGCACAGCA
GTTAGTACCTACTATTGATGGGAAGGGCCGTGTAGCAGCAATTGAGATTTTGTTAAACTCGCCACTGGTAACAGATTTAA
TCCAGCGTAACGAAATAGGCAGCCTCAAAGAAGCGATGAAAAAAGGAAAAGAGATGGGCATGCAAAGCTTTGATATGGCC
TTGTATGAGCTTTATAAAGAGCAGCGTATCTCCCTTGAGCAAGCGATTCATCATGCGGATTCACCTAACGACCTGAGATT
AATGATAAAACTTGATACGGAAGAGGGCACTGGGTTAGGCTCTCTGTCTAACGTATCAATAGATATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.341

98.378

0.584
  pilU Acinetobacter baylyi ADP1

57.766

99.189

0.573
  pilU Vibrio cholerae strain A1552

56.757

100

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.771

98.108

0.4
  pilT Pseudomonas aeruginosa PAK

41.449

93.243

0.386
  pilT Pseudomonas stutzeri DSM 10701

39.71

93.243

0.37
  pilT Legionella pneumophila strain Lp02

40.597

90.541

0.368
  pilT Legionella pneumophila strain ERS1305867

40.597

90.541

0.368
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362