Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QSG83_RS04495 Genome accession   NZ_CP127892
Coordinates   722342..723379 (-) Length   345 a.a.
NCBI ID   WP_096902267.1    Uniprot ID   A0A2A5PG39
Organism   Acinetobacter towneri strain SAAt401     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 717342..728379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG83_RS04465 (QSG83_04465) - 718124..718579 (+) 456 WP_004975314.1 bacteriohemerythrin -
  QSG83_RS04470 (QSG83_04470) - 718727..719812 (+) 1086 WP_317028372.1 hypothetical protein -
  QSG83_RS04475 (QSG83_04475) - 719809..720129 (+) 321 WP_096902265.1 RnfH family protein -
  QSG83_RS04480 (QSG83_04480) - 720186..720584 (-) 399 WP_178318246.1 outer membrane protein assembly factor BamE -
  QSG83_RS04485 (QSG83_04485) fur 720702..721139 (+) 438 WP_019838290.1 ferric iron uptake transcriptional regulator -
  QSG83_RS04490 (QSG83_04490) pilU 721202..722320 (-) 1119 WP_317028373.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QSG83_RS04495 (QSG83_04495) pilT 722342..723379 (-) 1038 WP_096902267.1 type IV pilus twitching motility protein PilT Machinery gene
  QSG83_RS04500 (QSG83_04500) - 723504..724193 (+) 690 WP_317028374.1 YggS family pyridoxal phosphate-dependent enzyme -
  QSG83_RS04505 (QSG83_04505) - 724554..724829 (-) 276 WP_104852403.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38254.77 Da        Isoelectric Point: 6.5056

>NTDB_id=845091 QSG83_RS04495 WP_096902267.1 722342..723379(-) (pilT) [Acinetobacter towneri strain SAAt401]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEIRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMIDFINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQSLLKKNGGGRVAAHEIMIGIPAVRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVSRGVISPQTARTVAKQSESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=845091 QSG83_RS04495 WP_096902267.1 722342..723379(-) (pilT) [Acinetobacter towneri strain SAAt401]
ATGGATATTACAGAATTATTGGCGTTCTCCGCTAAAAACGGCGCTTCGGATTTACACTTATCTGCGGGCATGCCACCACT
GATTCGTGTGGATGGTGAAATTCGTCGAATTAACTTACCAGCATTAGAACATAAAGAAGTACACAAATTAGTGTACGACA
TTATGAATGATAAACAACGTCGTGATTATGAAGAAAATCTAGAAACGGACTTTTCTTTTGAAGTCCCAGGCGTGGCTCGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCTTCTAAAGTTTTGACCCTTGA
AGATTTAGGCATGGGACAAATCTTTAAAGATATTTGTGAATATCCACGTGGTTTGGTATTGGTGACAGGCCCAACGGGTT
CAGGTAAATCAACCACACTTGCCGCGATGATTGACTTTATTAATGACAATCGCTACGACCATATTCTGACCGTCGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGTTTGGTTAACCAACGAGAAGTACACCGTGATACGCATGGCTTTAACGA
AGCATTACGTTCGGCACTGCGTGAAGACCCTGACATTATTCTAGTAGGTGAGATGCGTGACTTAGAAACCATTCGTTTGG
CACTGACTGCTGCTGAAACGGGTCACTTGGTATTTGGTACGCTACACACCACCTCTGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGTTGTCTGAATCTTTACAAGCCGTCATTTCACAATC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTAGCTGCGCATGAAATCATGATTGGTATTCCTGCAGTGCGTAACCTGATTC
GTGAAAACAAAGTAGCTCAAATGTACTCGGCAATTCAGACAGGTGCCAACTACGGTATGACTACCCTCGACCAGAGTTTG
AAGAACTTGGTATCGCGCGGTGTGATTAGCCCACAAACAGCTCGAACGGTTGCAAAACAATCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5PG39

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

93.913

100

0.939
  pilT Acinetobacter baumannii D1279779

93.623

100

0.936
  pilT Acinetobacter baumannii strain A118

93.623

100

0.936
  pilT Acinetobacter baylyi ADP1

85.756

99.71

0.855
  pilT Pseudomonas aeruginosa PAK

82.558

99.71

0.823
  pilT Pseudomonas stutzeri DSM 10701

82.267

99.71

0.82
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.986

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.696

100

0.687
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

97.391

0.67
  pilT Vibrio cholerae strain A1552

68.75

97.391

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.474

95.942

0.513
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

39.458

96.232

0.38