Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QSG83_RS04490 Genome accession   NZ_CP127892
Coordinates   721202..722320 (-) Length   372 a.a.
NCBI ID   WP_317028373.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SAAt401     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716202..727320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG83_RS04460 (QSG83_04460) - 717012..717860 (+) 849 WP_004975313.1 class II glutamine amidotransferase -
  QSG83_RS04465 (QSG83_04465) - 718124..718579 (+) 456 WP_004975314.1 bacteriohemerythrin -
  QSG83_RS04470 (QSG83_04470) - 718727..719812 (+) 1086 WP_317028372.1 hypothetical protein -
  QSG83_RS04475 (QSG83_04475) - 719809..720129 (+) 321 WP_096902265.1 RnfH family protein -
  QSG83_RS04480 (QSG83_04480) - 720186..720584 (-) 399 WP_178318246.1 outer membrane protein assembly factor BamE -
  QSG83_RS04485 (QSG83_04485) fur 720702..721139 (+) 438 WP_019838290.1 ferric iron uptake transcriptional regulator -
  QSG83_RS04490 (QSG83_04490) pilU 721202..722320 (-) 1119 WP_317028373.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QSG83_RS04495 (QSG83_04495) pilT 722342..723379 (-) 1038 WP_096902267.1 type IV pilus twitching motility protein PilT Machinery gene
  QSG83_RS04500 (QSG83_04500) - 723504..724193 (+) 690 WP_317028374.1 YggS family pyridoxal phosphate-dependent enzyme -
  QSG83_RS04505 (QSG83_04505) - 724554..724829 (-) 276 WP_104852403.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41800.04 Da        Isoelectric Point: 6.5793

>NTDB_id=845090 QSG83_RS04490 WP_317028373.1 721202..722320(-) (pilU) [Acinetobacter towneri strain SAAt401]
MDFNDLLNLMIQQQASDLFITADVEPSMKIHGQIVPVAKSKLTGEIIGQLLNSIMSEKQRKEFAETRECNFAITNREKTA
RFRVSAFQQRDEPGMVLRRIETKIPAMDDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAIVAQQLIPTIDGNSRRAAIEILINSPLVADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLAEEGGERLLHATSNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=845090 QSG83_RS04490 WP_317028373.1 721202..722320(-) (pilU) [Acinetobacter towneri strain SAAt401]
ATGGACTTTAATGATTTATTGAATTTAATGATTCAGCAACAAGCATCAGATTTGTTTATTACAGCCGATGTTGAACCTTC
CATGAAAATTCATGGACAAATTGTACCTGTGGCAAAAAGTAAGCTCACAGGTGAAATTATCGGTCAGTTACTTAACTCAA
TTATGAGTGAAAAACAGCGCAAGGAATTTGCCGAAACTCGTGAATGTAACTTTGCGATCACCAACCGTGAAAAAACAGCA
CGTTTCCGTGTCAGCGCATTCCAGCAACGAGATGAGCCGGGCATGGTACTGCGTCGGATTGAAACCAAAATTCCGGCCAT
GGATGACTTGAAACTTCCTCCTATTCTCAAAGATTTGGCGATGACCAAACGTGGCATCATCATTTTTGTAGGCGCAACGG
GTACAGGTAAATCGACTTCTTTGGCGTCTATTATTGGTTACCGCAACCAAAACTCTAAAGGTCATATCATCACCATTGAA
GACCCGATTGAATTTGTGCATGAACATGCGGGCTGTATTATTACCCAACGTGAAGTCGGGATTGATACCGATTCCTTTGA
AATTGCGTTAAAAAACACCTTGCGTCAAGCGCCTGATGTGATTCTGATTGGTGAGATTCGTTCACGCGAAACCATGGATT
ATGCAATTGCCTTTGCAGAAACGGGGCATTTGGTATTTGCCACCCTGCACGCCAACAACGCCAACCAAGCAATTGACCGT
ATTATTCACTTCTTTGAGGCAGACCGTCATAACCAGTTGTTCATGGATTTATCACTAAACCTGAAAGCAATTGTGGCACA
GCAGTTAATCCCAACCATTGATGGCAACTCACGACGAGCTGCGATTGAAATTTTAATTAACTCACCATTAGTTGCAGATT
TAATCCGTAAAGGTGATGTGCATGAAATTAAAGACCTAATGAAGCGTTCACGTGAATTGGGTATGCAAACCTTTGACCAA
GCTTTATATGACCTCTATAAAGCCAAGCAAATTAGTTATAAAGATGCACTCAAACACGCCGACTCGCCAAACGATTTGCG
TCTACAAATCAAACTTGCCGAAGAAGGCGGAGAGCGCTTGTTACATGCAACCTCGAATATTACTTTCGATGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

55.271

94.355

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain ERS1305867

41.124

90.86

0.374
  pilT Legionella pneumophila strain Lp02

41.124

90.86

0.374
  pilT Acinetobacter baumannii strain A118

40.233

92.204

0.371
  pilT Acinetobacter nosocomialis M2

40.233

92.204

0.371
  pilT Acinetobacter baumannii D1279779

40.233

92.204

0.371
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36
  pilT Acinetobacter baylyi ADP1

39.067

92.204

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36