Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QP512_RS04630 Genome accession   NZ_CP127407
Coordinates   1020034..1021071 (+) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas sp. 57     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1015034..1026071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP512_RS04605 soxR 1016310..1016744 (+) 435 WP_164082433.1 redox-sensitive transcriptional activator SoxR -
  QP512_RS04610 - 1016793..1017395 (-) 603 WP_164082432.1 short chain dehydrogenase -
  QP512_RS04615 - 1017494..1018369 (+) 876 WP_286071116.1 LysR family transcriptional regulator -
  QP512_RS04620 proC 1018429..1019250 (-) 822 WP_286071117.1 pyrroline-5-carboxylate reductase -
  QP512_RS04625 - 1019269..1019946 (-) 678 WP_164082429.1 YggS family pyridoxal phosphate-dependent enzyme -
  QP512_RS04630 pilT 1020034..1021071 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  QP512_RS04635 pilU 1021219..1022313 (+) 1095 WP_049411044.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP512_RS04640 - 1022657..1023640 (+) 984 WP_164082428.1 aldo/keto reductase -
  QP512_RS04645 - 1023696..1024595 (-) 900 WP_164082427.1 LysR family transcriptional regulator -
  QP512_RS04650 - 1024877..1025089 (+) 213 WP_164082426.1 DUF4287 domain-containing protein -
  QP512_RS04655 - 1025201..1025908 (+) 708 WP_029379885.1 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=844123 QP512_RS04630 WP_004147099.1 1020034..1021071(+) (pilT) [Stenotrophomonas sp. 57]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=844123 QP512_RS04630 WP_004147099.1 1020034..1021071(+) (pilT) [Stenotrophomonas sp. 57]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTTGACGGCGACGTTCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATCCCGTCGCTGGCGCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGTGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTAGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCATGGCTTCAACGA
AGCACTGCGCTCGGCGCTGCGCGAGGATCCGGACATCATCCTGGTGGGCGAACTTCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391