Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QP512_RS04635 Genome accession   NZ_CP127407
Coordinates   1021219..1022313 (+) Length   364 a.a.
NCBI ID   WP_049411044.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 57     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1016219..1027313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP512_RS04605 soxR 1016310..1016744 (+) 435 WP_164082433.1 redox-sensitive transcriptional activator SoxR -
  QP512_RS04610 - 1016793..1017395 (-) 603 WP_164082432.1 short chain dehydrogenase -
  QP512_RS04615 - 1017494..1018369 (+) 876 WP_286071116.1 LysR family transcriptional regulator -
  QP512_RS04620 proC 1018429..1019250 (-) 822 WP_286071117.1 pyrroline-5-carboxylate reductase -
  QP512_RS04625 - 1019269..1019946 (-) 678 WP_164082429.1 YggS family pyridoxal phosphate-dependent enzyme -
  QP512_RS04630 pilT 1020034..1021071 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  QP512_RS04635 pilU 1021219..1022313 (+) 1095 WP_049411044.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP512_RS04640 - 1022657..1023640 (+) 984 WP_164082428.1 aldo/keto reductase -
  QP512_RS04645 - 1023696..1024595 (-) 900 WP_164082427.1 LysR family transcriptional regulator -
  QP512_RS04650 - 1024877..1025089 (+) 213 WP_164082426.1 DUF4287 domain-containing protein -
  QP512_RS04655 - 1025201..1025908 (+) 708 WP_029379885.1 YitT family protein -
  QP512_RS04660 - 1025992..1026537 (-) 546 WP_164082424.1 DNA-3-methyladenine glycosylase I -
  QP512_RS04665 - 1026626..1027192 (+) 567 WP_005408377.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40537.55 Da        Isoelectric Point: 6.7715

>NTDB_id=844124 QP512_RS04635 WP_049411044.1 1021219..1022313(+) (pilU) [Stenotrophomonas sp. 57]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLATLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=844124 QP512_RS04635 WP_049411044.1 1021219..1022313(+) (pilU) [Stenotrophomonas sp. 57]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACGCCACTGACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCACAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTTGTCGGTGCTACCGGTACCGGTAAATCGACTTCGCTGGCGGCGA
TGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAG
GGTTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAGCTGGGAGGCTGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGGCCACGCTGCATGCCAACAATGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGGCGC
AACCAGCTGTTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTACAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACTTTCGACCAGAGCCTCTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCGCGCACCCTGTCGCAGGGCTTGGATGGCGTGGAGATCTCCGAGATCCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.994

98.352

0.679
  pilU Acinetobacter baylyi ADP1

64.368

95.604

0.615
  pilU Vibrio cholerae strain A1552

54.755

95.33

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363