Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QP482_RS04900 Genome accession   NZ_CP127406
Coordinates   1055941..1056978 (+) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas sp. As-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1050941..1061978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP482_RS04875 soxR 1052234..1052668 (+) 435 WP_032951587.1 redox-sensitive transcriptional activator SoxR -
  QP482_RS04880 - 1052706..1053308 (-) 603 WP_205405056.1 short chain dehydrogenase -
  QP482_RS04885 - 1053407..1054282 (+) 876 WP_180163775.1 LysR family transcriptional regulator -
  QP482_RS04890 proC 1054338..1055159 (-) 822 WP_205405055.1 pyrroline-5-carboxylate reductase -
  QP482_RS04895 - 1055176..1055853 (-) 678 WP_205405054.1 YggS family pyridoxal phosphate-dependent enzyme -
  QP482_RS04900 pilT 1055941..1056978 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  QP482_RS04905 pilU 1057058..1058221 (+) 1164 WP_049467229.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP482_RS04910 - 1058364..1059347 (+) 984 WP_099819251.1 aldo/keto reductase -
  QP482_RS04915 - 1059421..1060320 (-) 900 WP_205405053.1 LysR family transcriptional regulator -
  QP482_RS04920 - 1060728..1060940 (+) 213 WP_033830520.1 DUF4287 domain-containing protein -
  QP482_RS04925 - 1061267..1061974 (+) 708 WP_099819252.1 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=844105 QP482_RS04900 WP_004147099.1 1055941..1056978(+) (pilT) [Stenotrophomonas sp. As-14]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=844105 QP482_RS04900 WP_004147099.1 1055941..1056978(+) (pilT) [Stenotrophomonas sp. As-14]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCATCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGGATCAACATTCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCCTTCGAGATCCCGTCGCTGGCGCGC
TTCCGTGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTATTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGGCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGATACGCATGGTTTCAACGA
GGCGCTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAACTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCGGCGGCCAAAACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTCGGTGGCGGTCGTACTGCTGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAATACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391