Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QO259_RS20215 Genome accession   NZ_CP127244
Coordinates   4424964..4426004 (+) Length   346 a.a.
NCBI ID   WP_285951346.1    Uniprot ID   A0A9Y2D870
Organism   Salinicola sp. JS01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4419964..4431004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QO259_RS20185 (QO259_20185) sfsA 4420089..4420859 (+) 771 WP_285951342.1 DNA/RNA nuclease SfsA -
  QO259_RS20190 (QO259_20190) metW 4420877..4421473 (-) 597 WP_035476190.1 methionine biosynthesis protein MetW -
  QO259_RS20195 (QO259_20195) - 4421473..4422630 (-) 1158 WP_285951343.1 homoserine O-acetyltransferase -
  QO259_RS20200 (QO259_20200) - 4422711..4423301 (-) 591 WP_207036164.1 YggT family protein -
  QO259_RS20205 (QO259_20205) proC 4423330..4424169 (-) 840 WP_285951344.1 pyrroline-5-carboxylate reductase -
  QO259_RS20210 (QO259_20210) - 4424207..4424923 (-) 717 WP_285951345.1 YggS family pyridoxal phosphate-dependent enzyme -
  QO259_RS20215 (QO259_20215) pilT 4424964..4426004 (+) 1041 WP_285951346.1 type IV pilus twitching motility protein PilT Machinery gene
  QO259_RS20220 (QO259_20220) pilU 4426089..4427228 (+) 1140 WP_285951347.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QO259_RS20225 (QO259_20225) tyrS 4427321..4428520 (+) 1200 WP_285951348.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37952.38 Da        Isoelectric Point: 6.5087

>NTDB_id=843048 QO259_RS20215 WP_285951346.1 4424964..4426004(+) (pilT) [Salinicola sp. JS01]
MDITELLAFSAKQDASDLHLSAGLPPMIRVDGDMRRLNVPALDDSQVRRLIYDVMNDRQRRDFEADLEADFSFEVNGVAR
FRVNVFTQGRGAGAVFRIIPSLVRSLEELDLGDVFRRLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINSHRQEHILTIED
PIEFVHQSKRCLINQREVHRDTKGFAPALRSALREDPDVILVGELRDLETVRLALTAAETGHLVFATLHTTSAAKTIDRI
IDVFPGDEKGMVRSMLSESLQGVISQTLLKRAGEGGGRVAAHEILLGNSAIRNLIREGKVAQMYSAIQTGGNLGMQTLDA
ALARLVADGLVSRQEAQAKSRTGFES

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=843048 QO259_RS20215 WP_285951346.1 4424964..4426004(+) (pilT) [Salinicola sp. JS01]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAAGACGCCTCGGACCTGCACCTCTCGGCGGGCCTGCCGCCGAT
GATTCGCGTCGATGGCGACATGCGACGTCTCAACGTGCCGGCGCTCGACGACAGCCAGGTCAGGCGGCTTATCTATGATG
TGATGAACGATCGCCAGCGGCGCGACTTCGAAGCCGACCTGGAAGCCGATTTCTCGTTCGAGGTGAACGGCGTGGCCCGC
TTCCGGGTCAATGTCTTCACCCAAGGGCGCGGTGCCGGTGCGGTGTTCCGGATCATTCCCAGCCTCGTGCGTTCGCTCGA
AGAGCTCGATCTGGGCGATGTGTTCCGGCGCCTGGCGATGCTGCCGCGGGGGTTGGTGCTGGTCACCGGCCCGACCGGTT
CCGGCAAGAGCACCACGCTGGCGGCGATGATCGACTACATCAATAGTCATCGCCAGGAGCATATCCTGACCATCGAGGAC
CCGATCGAGTTCGTCCACCAGAGCAAGCGCTGTCTGATCAACCAGCGCGAGGTGCACCGTGACACCAAGGGCTTCGCGCC
GGCGCTGCGCTCCGCTCTGCGCGAGGACCCGGACGTGATTCTGGTGGGTGAGCTGCGCGACCTGGAGACGGTGCGTCTGG
CGCTGACCGCCGCCGAGACCGGGCACCTGGTCTTCGCTACCCTGCATACCACCTCGGCGGCCAAGACCATCGATCGTATC
ATCGATGTCTTCCCCGGTGACGAGAAGGGCATGGTGCGCTCGATGCTGTCGGAGTCGCTGCAGGGGGTGATCTCGCAGAC
GCTGCTCAAGCGTGCCGGCGAGGGCGGCGGTCGTGTCGCCGCGCACGAGATCCTCCTCGGCAACTCGGCGATCCGCAATC
TGATCCGCGAGGGCAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGTGGCAATCTGGGCATGCAGACCCTGGATGCC
GCGCTCGCCCGACTGGTCGCCGATGGCTTGGTCTCGCGTCAGGAAGCGCAGGCCAAATCCCGTACAGGGTTCGAGAGCTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.833

98.555

0.757
  pilT Pseudomonas stutzeri DSM 10701

76.246

98.555

0.751
  pilT Acinetobacter baylyi ADP1

74.854

98.844

0.74
  pilT Acinetobacter baumannii D1279779

74.487

98.555

0.734
  pilT Acinetobacter baumannii strain A118

74.487

98.555

0.734
  pilT Acinetobacter nosocomialis M2

74.194

98.555

0.731
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.142

97.977

0.668
  pilT Vibrio cholerae strain A1552

68.142

97.977

0.668
  pilT Legionella pneumophila strain Lp02

68.546

97.399

0.668
  pilT Legionella pneumophila strain ERS1305867

68.546

97.399

0.668
  pilT Neisseria meningitidis 8013

65.23

100

0.656
  pilT Neisseria gonorrhoeae MS11

65.103

98.555

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.518

95.954

0.523
  pilU Vibrio cholerae strain A1552

44.048

97.11

0.428
  pilU Pseudomonas stutzeri DSM 10701

41.964

97.11

0.408
  pilU Acinetobacter baylyi ADP1

41.003

97.977

0.402