Detailed information NTG0843049

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QO259_RS20220
• Genome accession: NZ_CP127244
• Coordinates: 4426089..4427228 (+)
• Length: 379 a.a.
• NCBI ID: WP_285951347.1
• Uniprot ID: A0A9Y2D682
• Organism: Salinicola sp. JS01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4421089..4432228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QO259_RS20195 (QO259_20195)	-	4421473..4422630 (-)	1158	WP_285951343.1	homoserine O-acetyltransferase	-
QO259_RS20200 (QO259_20200)	-	4422711..4423301 (-)	591	WP_207036164.1	YggT family protein	-
QO259_RS20205 (QO259_20205)	proC	4423330..4424169 (-)	840	WP_285951344.1	pyrroline-5-carboxylate reductase	-
QO259_RS20210 (QO259_20210)	-	4424207..4424923 (-)	717	WP_285951345.1	YggS family pyridoxal phosphate-dependent enzyme	-
QO259_RS20215 (QO259_20215)	pilT	4424964..4426004 (+)	1041	WP_285951346.1	type IV pilus twitching motility protein PilT	Machinery gene
QO259_RS20220 (QO259_20220)	pilU	4426089..4427228 (+)	1140	WP_285951347.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QO259_RS20225 (QO259_20225)	tyrS	4427321..4428520 (+)	1200	WP_285951348.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 41800.71 Da
• Isoelectric Point: 5.4569

>NTDB_id=843049 QO259_RS20220 WP_285951347.1 4426089..4427228(+) (pilU) [Salinicola sp. JS01]
MTPHQWFEQLLDIMIAKQASDLLISTGAPPCLKLDGKLVALGDVPLRPEQVRELVATTLPPGGREDFEREHEANFALSLS
GKGRFRVSAFFQRSQQAMVVRRIAYDIPALESLGLPEAVATLPTIRRGLILVVGGTGSGKSTTLASMIQHRNRTSGGHII
CIEDPIEYMHPHQRGIINQREVGIDTESYEIGLRNALRQAPDVIVIGEIRSRDTMELALAFAETGHLCLATLHANNADQA
VERILNLFPSERHDQVWMDLSLNLRAVVAQQLLPRRDGEGRCLATEVMLQSPLISELIRRGEISELKPLIRRSRELGMQT
FDQSLYALYSQGLIDERVALSHADSANDLRMLIKYGEAGASGEAGGETGEGRFTLADDD

Nucleotide

• Length: 1140 bp

>NTDB_id=843049 QO259_RS20220 WP_285951347.1 4426089..4427228(+) (pilU) [Salinicola sp. JS01]
ATGACGCCGCACCAATGGTTTGAACAGCTTCTCGATATCATGATCGCCAAGCAGGCCTCCGACCTGCTGATTTCCACCGG
CGCACCGCCCTGTCTCAAGCTCGATGGCAAGCTGGTCGCGCTGGGTGACGTGCCGCTGCGCCCCGAACAGGTGCGCGAGC
TGGTGGCGACCACCTTGCCGCCAGGCGGGCGCGAGGATTTCGAGCGCGAGCATGAGGCCAACTTTGCGCTCAGCTTGAGC
GGCAAGGGGCGGTTCCGGGTCAGTGCCTTCTTCCAGCGCAGTCAGCAAGCGATGGTGGTGCGCCGCATCGCCTATGACAT
TCCAGCGCTCGAGAGCCTGGGGCTTCCCGAGGCGGTCGCCACGCTGCCGACCATCCGGCGCGGCCTCATACTGGTGGTCG
GGGGGACCGGCAGCGGCAAGTCCACCACCCTGGCGTCGATGATCCAGCACCGCAACCGCACCAGCGGTGGGCATATCATC
TGCATCGAAGACCCGATCGAGTACATGCATCCACATCAGCGCGGGATCATCAATCAGCGCGAGGTGGGCATCGACACCGA
GTCCTACGAGATCGGCCTCAGGAATGCGCTGCGTCAGGCGCCGGACGTGATCGTGATCGGCGAGATTCGCAGTCGCGACA
CCATGGAGCTGGCACTCGCCTTCGCCGAGACCGGGCATCTGTGTCTCGCCACACTCCACGCCAACAACGCCGACCAGGCG
GTGGAGCGGATCCTCAATCTGTTCCCGTCCGAGCGCCACGACCAAGTGTGGATGGATCTGTCGCTCAACCTGCGCGCCGT
CGTGGCTCAGCAACTGCTGCCACGGCGCGATGGCGAGGGGCGCTGTCTGGCCACCGAGGTGATGCTGCAGTCGCCGCTGA
TCAGCGAGCTGATCAGGCGCGGCGAGATCAGTGAACTCAAGCCGCTGATCCGGCGTTCGCGGGAGCTGGGCATGCAGACC
TTCGATCAGTCGCTCTACGCCCTCTATAGCCAGGGATTGATCGATGAGCGCGTTGCGCTCTCTCACGCCGACTCCGCCAA
CGATCTGCGCATGCTGATCAAGTATGGCGAAGCGGGGGCGTCGGGCGAGGCTGGCGGCGAGACTGGCGAAGGCCGGTTCA
CCCTGGCCGACGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.15	98.681	0.554
pilU	Vibrio cholerae strain A1552	55.46	91.821	0.509
pilU	Acinetobacter baylyi ADP1	53.693	92.876	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.818	95.515	0.409
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.896	86.28	0.361
pilT	Vibrio cholerae strain A1552	41.896	86.28	0.361