Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   COW1_RS13970 Genome accession   NZ_AP024239
Coordinates   2950716..2951753 (+) Length   345 a.a.
NCBI ID   WP_096367367.1    Uniprot ID   A0A1Z4VUR9
Organism   Thiohalobacter sp. COW1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2945716..2956753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COW1_RS13945 (TspCOW1_27720) - 2945912..2947888 (-) 1977 WP_096367371.1 dynamin family protein -
  COW1_RS13950 (TspCOW1_27730) - 2948086..2948379 (-) 294 WP_096367370.1 DUF167 family protein -
  COW1_RS13955 (TspCOW1_27740) - 2948381..2948953 (-) 573 WP_096367369.1 YggT family protein -
  COW1_RS13960 (TspCOW1_27750) proC 2948950..2949780 (-) 831 WP_201344525.1 pyrroline-5-carboxylate reductase -
  COW1_RS13965 (TspCOW1_27760) - 2949839..2950558 (-) 720 WP_370590868.1 YggS family pyridoxal phosphate-dependent enzyme -
  COW1_RS13970 (TspCOW1_27770) pilT 2950716..2951753 (+) 1038 WP_096367367.1 type IV pilus twitching motility protein PilT Machinery gene
  COW1_RS13975 (TspCOW1_27780) pilU 2951946..2953115 (+) 1170 WP_096367366.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  COW1_RS13980 (TspCOW1_27790) - 2953131..2953412 (-) 282 WP_201344526.1 SelT/SelW/SelH family protein -
  COW1_RS13985 (TspCOW1_27800) - 2953478..2954356 (-) 879 WP_201344527.1 dihydroorotate dehydrogenase electron transfer subunit -
  COW1_RS13990 (TspCOW1_27810) - 2954596..2955897 (-) 1302 WP_096367363.1 dihydroorotase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38182.84 Da        Isoelectric Point: 6.7278

>NTDB_id=84249 COW1_RS13970 WP_096367367.1 2950716..2951753(+) (pilT) [Thiohalobacter sp. COW1]
MDIAELLAFGVKNNASDLHLSAGLPPMIRVDGDIRRINVPALEHKEVHALVYDIMNDKQRKDFEEFLETDFSFEIPNLAR
FRVNAFNHNRGAGAVFRTIPSKILSLEDLGCPPVFKDIADQPRGIVLVTGPTGSGKSTTLAAMMNHVNETRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKSMVRSMLSESLRAVISQTLLKKTGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGQAAGMQTLDQNL
QEIVQKGLVSRQDARAKAANKELFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=84249 COW1_RS13970 WP_096367367.1 2950716..2951753(+) (pilT) [Thiohalobacter sp. COW1]
ATGGATATCGCAGAACTGCTCGCCTTCGGGGTCAAGAACAACGCCTCCGACCTGCATCTCTCCGCCGGGCTGCCGCCCAT
GATCCGCGTCGACGGCGACATCCGCCGCATCAATGTGCCGGCGTTGGAGCACAAGGAAGTCCACGCGCTGGTCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCGTTCGAGATCCCGAACCTGGCGCGC
TTCCGTGTCAACGCCTTCAACCACAATCGCGGCGCCGGCGCGGTGTTCCGTACCATCCCCTCCAAGATACTGAGCCTGGA
GGACCTCGGCTGCCCGCCGGTGTTCAAGGATATCGCCGATCAGCCGCGCGGCATCGTGCTGGTCACCGGCCCGACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATGAACCACGTCAACGAGACCCGCTACGAACACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTACACGAATCCAAGAAATGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCGCCGA
GGCGCTGCGTTCCGCCCTGCGCGAGGATCCGGACATCGTGCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACTCTGCATACCAGTTCCGCGGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCCGCGGCGGAGAAATCCATGGTCCGGTCCATGCTCTCCGAGTCGCTGCGCGCGGTCATTTCCCAGAC
GCTGCTCAAGAAGACCGGCGGCGGGCGTGTCGCCGCGCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCCCAGATGTACTCGGCCATCCAGACCGGTCAGGCTGCCGGCATGCAGACCCTGGATCAGAACCTG
CAGGAGATCGTGCAGAAAGGACTCGTCAGCCGGCAGGATGCCCGCGCCAAGGCGGCCAACAAGGAGTTGTTCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z4VUR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

99.71

0.82
  pilT Pseudomonas stutzeri DSM 10701

81.977

99.71

0.817
  pilT Acinetobacter nosocomialis M2

81.105

99.71

0.809
  pilT Acinetobacter baumannii D1279779

81.105

99.71

0.809
  pilT Acinetobacter baumannii strain A118

81.105

99.71

0.809
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Legionella pneumophila strain Lp02

76.744

99.71

0.765
  pilT Legionella pneumophila strain ERS1305867

76.744

99.71

0.765
  pilT Neisseria meningitidis 8013

69.388

99.42

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.681

0.69
  pilT Vibrio cholerae strain A1552

70.623

97.681

0.69
  pilT Neisseria gonorrhoeae MS11

69.096

99.42

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397

Multiple sequence alignment