Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   COW1_RS13975 Genome accession   NZ_AP024239
Coordinates   2951946..2953115 (+) Length   389 a.a.
NCBI ID   WP_096367366.1    Uniprot ID   A0A1Z4VV67
Organism   Thiohalobacter sp. COW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2946946..2958115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COW1_RS13950 (TspCOW1_27730) - 2948086..2948379 (-) 294 WP_096367370.1 DUF167 family protein -
  COW1_RS13955 (TspCOW1_27740) - 2948381..2948953 (-) 573 WP_096367369.1 YggT family protein -
  COW1_RS13960 (TspCOW1_27750) proC 2948950..2949780 (-) 831 WP_201344525.1 pyrroline-5-carboxylate reductase -
  COW1_RS13965 (TspCOW1_27760) - 2949839..2950558 (-) 720 WP_370590868.1 YggS family pyridoxal phosphate-dependent enzyme -
  COW1_RS13970 (TspCOW1_27770) pilT 2950716..2951753 (+) 1038 WP_096367367.1 type IV pilus twitching motility protein PilT Machinery gene
  COW1_RS13975 (TspCOW1_27780) pilU 2951946..2953115 (+) 1170 WP_096367366.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  COW1_RS13980 (TspCOW1_27790) - 2953131..2953412 (-) 282 WP_201344526.1 SelT/SelW/SelH family protein -
  COW1_RS13985 (TspCOW1_27800) - 2953478..2954356 (-) 879 WP_201344527.1 dihydroorotate dehydrogenase electron transfer subunit -
  COW1_RS13990 (TspCOW1_27810) - 2954596..2955897 (-) 1302 WP_096367363.1 dihydroorotase -
  COW1_RS13995 (TspCOW1_27820) - 2955897..2956883 (-) 987 WP_096367362.1 aspartate carbamoyltransferase catalytic subunit -
  COW1_RS14000 (TspCOW1_27830) pyrR 2956880..2957383 (-) 504 WP_096367361.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  COW1_RS14005 (TspCOW1_27840) ruvX 2957383..2957796 (-) 414 WP_096367360.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43554.81 Da        Isoelectric Point: 6.3222

>NTDB_id=84250 COW1_RS13975 WP_096367366.1 2951946..2953115(+) (pilU) [Thiohalobacter sp. COW1]
MAQDLKTLLMLVVRKKASDLFITAGVPPSIKVDGKIVAAAREPLAPAEARDMVMATVPEHLRDEFNRTHECNFALSETGL
GRFRVSAFYQRNHVGMVLRRIETRIPDFEGLNLPPVLEKLAMTKRGLVLFVGATGTGKSTSLAAMLGYRNHHGNGHIVTL
EDPIEFIHQHDGCIVTQREVGLDTDSYEIGLRNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLCLSTLHANNANQALD
RIINFFPEDRRDQLLMDLSLNLKAMIAQQLIPTVDGKGRRAAVEVLINSPLASEYIRNGEIHRLKDLMKRSREQGMQTFD
QALFELYKAGDISYDNALHYADSPNEVRLMIKLDEEDVDKLGDAIADLEIDNSDQYALSGHKRGEKKRT

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=84250 COW1_RS13975 WP_096367366.1 2951946..2953115(+) (pilU) [Thiohalobacter sp. COW1]
ATGGCACAGGATCTCAAGACACTGCTCATGCTGGTGGTCAGGAAGAAGGCCTCCGATCTGTTCATCACCGCCGGTGTGCC
GCCCAGCATCAAGGTCGACGGCAAGATCGTGGCTGCGGCCAGGGAGCCGCTGGCCCCGGCCGAGGCCCGGGACATGGTCA
TGGCGACGGTGCCCGAGCATCTGCGCGATGAGTTCAACCGTACCCATGAATGCAACTTCGCCCTGAGCGAGACCGGCCTC
GGACGCTTCCGTGTCAGCGCCTTCTACCAGCGCAACCACGTCGGCATGGTGCTGCGTCGGATCGAGACCCGGATCCCCGA
CTTCGAGGGGCTGAACCTGCCGCCCGTGCTCGAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGCTGTTCGTGGGCGCGA
CCGGCACCGGCAAGTCCACCTCGCTGGCCGCCATGCTCGGCTATCGCAACCATCACGGCAACGGCCACATCGTCACCCTG
GAAGACCCCATCGAGTTCATCCACCAGCATGACGGCTGCATTGTCACCCAGCGGGAGGTCGGGCTGGACACCGATTCCTA
CGAGATCGGCCTGCGCAACACCCTGCGTCAGGCCCCGGATGTGATCCTGATCGGCGAGATCCGCAGCCGCGAGACCATGG
ACTACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGTCTGTCCACGCTGCACGCCAACAACGCCAACCAGGCCCTGGAC
CGCATCATCAACTTCTTCCCCGAGGACCGCCGCGACCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGCCATGATCGC
CCAGCAGCTGATTCCCACCGTTGACGGCAAGGGCCGTCGTGCCGCCGTCGAGGTGCTGATCAACAGTCCACTGGCCTCGG
AATACATCCGCAATGGCGAGATCCATCGTCTGAAGGACCTGATGAAGCGCTCCCGCGAGCAGGGCATGCAGACCTTTGAT
CAGGCCCTGTTCGAACTCTACAAGGCCGGCGACATCAGCTATGACAACGCCCTGCACTACGCCGACTCGCCCAACGAGGT
GCGGTTGATGATCAAGCTGGACGAAGAGGATGTGGACAAGCTCGGCGATGCCATCGCCGACCTGGAGATCGACAACTCGG
ATCAGTACGCCCTGTCCGGCCACAAGCGCGGGGAGAAGAAGCGGACTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z4VV67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.742

95.63

0.648
  pilU Acinetobacter baylyi ADP1

64.689

91.003

0.589
  pilU Vibrio cholerae strain A1552

58.989

91.517

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.399

88.175

0.365

Multiple sequence alignment