Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JN178_RS15240 Genome accession   NZ_AP024235
Coordinates   3438193..3439236 (-) Length   347 a.a.
NCBI ID   WP_159626757.1    Uniprot ID   -
Organism   Alteromonas sp. KC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3433193..3444236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN178_RS15210 (KUC3_30050) - 3433835..3434563 (+) 729 WP_202262269.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JN178_RS15215 (KUC3_30060) gshB 3434712..3435665 (+) 954 WP_202262270.1 glutathione synthase -
  JN178_RS15220 (KUC3_30070) - 3435715..3436281 (+) 567 WP_202262271.1 YqgE/AlgH family protein -
  JN178_RS15225 (KUC3_30080) ruvX 3436274..3436705 (+) 432 WP_159626762.1 Holliday junction resolvase RuvX -
  JN178_RS15230 (KUC3_30090) - 3436712..3437041 (-) 330 WP_202262272.1 DUF2007 domain-containing protein -
  JN178_RS15235 (KUC3_30100) pilU 3437071..3438183 (-) 1113 WP_202262273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN178_RS15240 (KUC3_30110) pilT 3438193..3439236 (-) 1044 WP_159626757.1 type IV pilus twitching motility protein PilT Machinery gene
  JN178_RS15245 (KUC3_30120) - 3439264..3439962 (+) 699 WP_202262274.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN178_RS15250 (KUC3_30130) proC 3440028..3440849 (+) 822 WP_159626753.1 pyrroline-5-carboxylate reductase -
  JN178_RS15255 (KUC3_30140) - 3440871..3441410 (+) 540 WP_202262275.1 YggT family protein -
  JN178_RS15260 (KUC3_30150) - 3441445..3441885 (+) 441 WP_202262276.1 DUF4426 domain-containing protein -
  JN178_RS15265 (KUC3_30160) - 3441948..3442622 (-) 675 WP_202262277.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38389.18 Da        Isoelectric Point: 6.5681

>NTDB_id=84189 JN178_RS15240 WP_159626757.1 3438193..3439236(-) (pilT) [Alteromonas sp. KC3]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHALVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLINQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAEKSIDKKSMSGL

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=84189 JN178_RS15240 WP_159626757.1 3438193..3439236(-) (pilT) [Alteromonas sp. KC3]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCAGGCTTACCCCCAAT
CATTCGTGTCGATGGAGAGATGCGTAAACTGAATGTACCCGCCCTAGATCACAAACAGGTGCATGCGCTCGTTTATGAAA
TTATGAACGACATGCAGCGTAAAGAATACGAAGAAAACTTAGAAACCGATTTCTCGTTTGAAGTAAACGGCTTGTCGCGT
TTTCGTGTTAACGCTTTTGTTCAAAACCGTGGTGCCGCTGCGGTTTTGCGTACCATCCCAAGTAAAGTGCTGACACTTGA
CGATTTAGGCGCTCCAGAAATTTTCAAAGAAATCATCAACCAGCCTACGGGAATTGTGCTGGTAACCGGCGCTACAGGTT
CGGGTAAAAGTACCACGCTTGCTGCCATGGTTGACCATATCAATACGCATAAACGCGAACATATTTTAACCATTGAAGAC
CCAATCGAATTTGTGCACGACAACAAGCTGAGCTTGATTAATCAGCGTGAAGTACATCGCGACACTCATTCATTTAATAA
CGCACTTCGTTCAGCATTGCGTGAAGACCCTGATGTCATTCTCGTTGGTGAGCTACGTGATCTTGAAACTATTCGATTGG
CTATTTCTGCCGCTGAAACGGGCCACTTGGTATTTGGTACACTTCACACCAACTCTGCACCCAAAACCATAGACCGTATT
ATCGATGTATTCCCAGCAGAGGAAAAAGCCATGGTTCGCTCTATGCTGTCTGAGTCATTGCGCGCGGTTATCTCGCAAAC
ACTGCTGAAAAAAGTGGGTGGGGGACGCGTGGCGGCACACGAAATTATGGTGGGTATACCGGCTATACGTAACCTTATTC
GTGAAGATAAAGTGCCTCAGATGTATTCTGTTATTCAAACTGGGCAAGCCCACGGTATGCAAACCATGGATCAATGCTTA
CAGCGTTTGGTCGCAATGGGTGTCATTACTAAGCAAGATGCTGCCGAAAAATCTATCGATAAAAAATCGATGAGTGGTCT
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.144

96.254

0.752
  pilT Acinetobacter baumannii strain A118

78.144

96.254

0.752
  pilT Acinetobacter nosocomialis M2

77.844

96.254

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.74

97.406

0.738
  pilT Pseudomonas aeruginosa PAK

75.148

97.406

0.732
  pilT Acinetobacter baylyi ADP1

76.596

94.813

0.726
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.118

0.709
  pilT Vibrio cholerae strain A1552

72.997

97.118

0.709
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Neisseria meningitidis 8013

65.607

99.712

0.654
  pilT Neisseria gonorrhoeae MS11

65.318

99.712

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.586

100

0.527
  pilU Pseudomonas stutzeri DSM 10701

41.092

100

0.412
  pilU Acinetobacter baylyi ADP1

41.716

97.406

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment