Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JN178_RS15235 Genome accession   NZ_AP024235
Coordinates   3437071..3438183 (-) Length   370 a.a.
NCBI ID   WP_202262273.1    Uniprot ID   -
Organism   Alteromonas sp. KC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3432071..3443183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN178_RS15205 (KUC3_30040) - 3432149..3432991 (-) 843 WP_202262268.1 mechanosensitive ion channel domain-containing protein -
  JN178_RS15210 (KUC3_30050) - 3433835..3434563 (+) 729 WP_202262269.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JN178_RS15215 (KUC3_30060) gshB 3434712..3435665 (+) 954 WP_202262270.1 glutathione synthase -
  JN178_RS15220 (KUC3_30070) - 3435715..3436281 (+) 567 WP_202262271.1 YqgE/AlgH family protein -
  JN178_RS15225 (KUC3_30080) ruvX 3436274..3436705 (+) 432 WP_159626762.1 Holliday junction resolvase RuvX -
  JN178_RS15230 (KUC3_30090) - 3436712..3437041 (-) 330 WP_202262272.1 DUF2007 domain-containing protein -
  JN178_RS15235 (KUC3_30100) pilU 3437071..3438183 (-) 1113 WP_202262273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN178_RS15240 (KUC3_30110) pilT 3438193..3439236 (-) 1044 WP_159626757.1 type IV pilus twitching motility protein PilT Machinery gene
  JN178_RS15245 (KUC3_30120) - 3439264..3439962 (+) 699 WP_202262274.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN178_RS15250 (KUC3_30130) proC 3440028..3440849 (+) 822 WP_159626753.1 pyrroline-5-carboxylate reductase -
  JN178_RS15255 (KUC3_30140) - 3440871..3441410 (+) 540 WP_202262275.1 YggT family protein -
  JN178_RS15260 (KUC3_30150) - 3441445..3441885 (+) 441 WP_202262276.1 DUF4426 domain-containing protein -
  JN178_RS15265 (KUC3_30160) - 3441948..3442622 (-) 675 WP_202262277.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40752.69 Da        Isoelectric Point: 6.0491

>NTDB_id=84188 JN178_RS15235 WP_202262273.1 3437071..3438183(-) (pilU) [Alteromonas sp. KC3]
MLDTLLQKMVDSNASDLFITAGFPVSAKINGKLTPITEGVSSEEASLALVHEAMSEKQRDEFHTSKECNFAIVREGIGRF
RCSAFWQRDQAGMVVRRIVTQIPKADDLGLPTVLKDIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTKDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=84188 JN178_RS15235 WP_202262273.1 3437071..3438183(-) (pilU) [Alteromonas sp. KC3]
ATGCTAGATACCTTATTACAAAAAATGGTCGACTCGAACGCGTCCGATTTATTTATCACCGCAGGTTTTCCTGTCAGCGC
AAAAATTAACGGCAAGCTAACGCCAATCACCGAAGGTGTGTCGTCTGAAGAGGCCTCATTAGCACTGGTTCATGAAGCCA
TGAGTGAAAAACAGCGTGACGAGTTTCACACGTCGAAAGAGTGTAACTTCGCTATCGTTAGAGAAGGTATTGGCCGTTTC
CGCTGCTCTGCTTTTTGGCAACGTGATCAGGCGGGCATGGTGGTCCGTCGTATTGTGACTCAAATCCCAAAAGCAGACGA
TTTAGGTCTGCCAACTGTCCTGAAAGACATCATTATGGCCAAGCGGGGCTTGGTGCTTTTCGTGGGGGGCACGGGTACGG
GTAAATCAACGTCACTCGCGGCACTCATTGGCCATAGAAATGAAAACTCTCACGGTCATATATTAACGATTGAAGACCCG
ATTGAATTTGTACACGAGCACAAAAATTGTGTTATCACTCAGCGAGAAGTAGGCATAGATACGCAGTCATTTGATGACGC
GCTTAAAAGCTCATTACGTCAAGCTCCAGATGTCATCCTCATTGGTGAGATTCGCTCAATGGAGACTATGGAGTACGCCA
TGTCTTTTGCTGATACTGGACACTTGTGTGTGGCAACACTTCACGCGAATAATGCCAATCAGGCTATCGAGCGCATTATG
CACCTTGCGCCAAAAGATCAGCACGACAAACTTCGTTTTGACTTAAGTCTGAATATTCGAGCAATTGTAGCGCAGCAGTT
AGTGCCGACACCTGATGGAAAAGGTCGTGTGGCGGCCATTGAGATTTTACTTAACTCGCCATTGGTACGCGACTTAATAC
AGCGCAATGAAATCGGTGCGCTAAAAGAAGCGATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGTTTTGATATGGCGCTA
TACGATTTGTATAAAGCGGGCAAAATTGACATGGACCAGGCGCTTCATCATGCCGACTCGCCGAACGATTTACGCCTAAT
GATTAAGCTTGATACGAAAGACGGTTCAAGCTTGGGCACATTGTCTAATGTCTCAATTGACATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Vibrio cholerae strain A1552

55.978

99.459

0.557
  pilU Acinetobacter baylyi ADP1

56.438

98.649

0.557
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.982

92.432

0.397
  pilT Acinetobacter nosocomialis M2

40

91.892

0.368
  pilT Acinetobacter baumannii D1279779

40

91.892

0.368
  pilT Acinetobacter baumannii strain A118

40

91.892

0.368

Multiple sequence alignment