Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L6421_RS02515 Genome accession   NZ_AP024202
Coordinates   579136..580173 (-) Length   345 a.a.
NCBI ID   WP_237262853.1    Uniprot ID   -
Organism   Thiomicrorhabdus immobilis strain Am19     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 574136..585173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6421_RS02495 (THMIRHAM_04950) - 574909..575775 (+) 867 WP_237262846.1 type IV pilus biogenesis/stability protein PilW -
  L6421_RS02500 (THMIRHAM_04960) - 575762..576886 (+) 1125 WP_237262848.1 murein L,D-transpeptidase family protein -
  L6421_RS02505 (THMIRHAM_04970) - 577002..577952 (-) 951 WP_237262850.1 M14 family zinc carboxypeptidase -
  L6421_RS02510 (THMIRHAM_04980) pilU 577973..579124 (-) 1152 WP_237262851.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L6421_RS02515 (THMIRHAM_04990) pilT 579136..580173 (-) 1038 WP_237262853.1 type IV pilus twitching motility protein PilT Machinery gene
  L6421_RS02520 (THMIRHAM_05000) - 580327..580932 (+) 606 WP_237262854.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  L6421_RS02525 (THMIRHAM_05010) - 581039..581479 (-) 441 WP_237262856.1 DNA polymerase III subunit chi -
  L6421_RS02530 (THMIRHAM_05020) - 581494..582984 (-) 1491 WP_237262858.1 leucyl aminopeptidase -
  L6421_RS02535 (THMIRHAM_05030) lptF 583087..584199 (+) 1113 WP_255695493.1 LPS export ABC transporter permease LptF -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38402.07 Da        Isoelectric Point: 6.1145

>NTDB_id=83995 L6421_RS02515 WP_237262853.1 579136..580173(-) (pilT) [Thiomicrorhabdus immobilis strain Am19]
MDITQLLEFSVQQGASDLHLSTGQPPIIRINGDIQRVEAPNMEAEQLQNMIYDVMNDEQRRGFESELEADFSFELPKIAR
YRANVFMHNRGIGAVFRTIPSDIITLEELGAPEIFKDISSFPRGLVLVTGPTGSGKSTTLAAMIDYINKKDPAHILTVED
PIEFVHQPIRALINQREVHRDTKGFNNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRSMLSESLRAVISQALLKKTTGGRIAAHEIMLVNSAIRNLIREAKVPQMYSVIQTSKKLGMQTMDQNL
QDLVAKNLITREDARSKAANKAAFA

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=83995 L6421_RS02515 WP_237262853.1 579136..580173(-) (pilT) [Thiomicrorhabdus immobilis strain Am19]
ATGGATATTACCCAGCTTCTTGAATTTAGTGTTCAACAAGGTGCCTCAGATTTACATCTATCTACAGGCCAACCACCGAT
TATCCGTATAAACGGCGATATTCAGCGTGTTGAAGCGCCTAATATGGAGGCTGAGCAACTCCAAAACATGATTTACGATG
TGATGAATGATGAACAGCGCCGAGGGTTTGAATCGGAACTTGAAGCCGACTTTTCCTTTGAACTGCCGAAAATCGCTCGT
TACCGTGCCAATGTATTCATGCACAATCGCGGAATTGGAGCGGTATTTCGTACTATTCCTAGCGATATCATTACCCTTGA
AGAACTCGGTGCGCCAGAGATATTCAAAGACATTTCCAGTTTTCCAAGAGGCTTGGTATTGGTTACCGGGCCTACCGGTT
CGGGTAAATCAACCACCTTGGCCGCAATGATTGATTACATCAATAAAAAAGACCCCGCACACATCTTGACGGTTGAAGAT
CCGATTGAATTTGTACACCAGCCGATTCGAGCACTGATAAACCAACGTGAAGTGCATAGAGATACCAAAGGTTTCAACAA
TGCCTTACGTTCCGCATTACGTGAAGACCCTGACGTTATTTTAGTGGGTGAGATGCGTGATTTAGAAACCATCCGTCTCG
CTCTGACCGCTGCAGAAACCGGGCACTTGGTTTTTGGTACTTTGCATACCAGTTCTGCTGCAAAAACCATTGACCGTATA
ATCGATGTTTTTCCTGCGGAAGAAAAAGAGATGGTGCGTTCGATGCTGTCTGAATCTTTAAGAGCGGTTATTTCGCAAGC
CCTGCTCAAGAAAACCACGGGGGGCCGAATAGCCGCACATGAAATCATGCTGGTCAATTCAGCCATCCGTAACTTAATAC
GTGAAGCCAAGGTGCCACAAATGTATTCGGTTATTCAAACCTCAAAAAAATTGGGCATGCAAACCATGGACCAAAACTTG
CAAGACCTAGTCGCTAAAAACCTGATAACTCGAGAAGATGCGCGTTCAAAAGCCGCAAATAAAGCAGCATTTGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

71.221

99.71

0.71
  pilT Pseudomonas aeruginosa PAK

71.302

97.971

0.699
  pilT Pseudomonas stutzeri DSM 10701

69.477

99.71

0.693
  pilT Acinetobacter baumannii D1279779

69.477

99.71

0.693
  pilT Acinetobacter baumannii strain A118

69.477

99.71

0.693
  pilT Acinetobacter nosocomialis M2

69.186

99.71

0.69
  pilT Neisseria meningitidis 8013

65.896

100

0.661
  pilT Neisseria gonorrhoeae MS11

65.607

100

0.658
  pilT Legionella pneumophila strain Lp02

65.698

99.71

0.655
  pilT Legionella pneumophila strain ERS1305867

65.698

99.71

0.655
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.681

0.641
  pilT Vibrio cholerae strain A1552

65.579

97.681

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.445

100

0.516
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406
  pilU Vibrio cholerae strain A1552

39.528

98.261

0.388
  pilU Acinetobacter baylyi ADP1

38.235

98.551

0.377

Multiple sequence alignment