Detailed information NTG0083994

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L6421_RS02510
• Genome accession: NZ_AP024202
• Coordinates: 577973..579124 (-)
• Length: 383 a.a.
• NCBI ID: WP_237262851.1
• Uniprot ID: -
• Organism: Thiomicrorhabdus immobilis strain Am19
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 572973..584124

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6421_RS02495 (THMIRHAM_04950)	-	574909..575775 (+)	867	WP_237262846.1	type IV pilus biogenesis/stability protein PilW	-
L6421_RS02500 (THMIRHAM_04960)	-	575762..576886 (+)	1125	WP_237262848.1	murein L,D-transpeptidase family protein	-
L6421_RS02505 (THMIRHAM_04970)	-	577002..577952 (-)	951	WP_237262850.1	M14 family zinc carboxypeptidase	-
L6421_RS02510 (THMIRHAM_04980)	pilU	577973..579124 (-)	1152	WP_237262851.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L6421_RS02515 (THMIRHAM_04990)	pilT	579136..580173 (-)	1038	WP_237262853.1	type IV pilus twitching motility protein PilT	Machinery gene
L6421_RS02520 (THMIRHAM_05000)	-	580327..580932 (+)	606	WP_237262854.1	Wzz/FepE/Etk N-terminal domain-containing protein	-
L6421_RS02525 (THMIRHAM_05010)	-	581039..581479 (-)	441	WP_237262856.1	DNA polymerase III subunit chi	-
L6421_RS02530 (THMIRHAM_05020)	-	581494..582984 (-)	1491	WP_237262858.1	leucyl aminopeptidase	-

Sequence

Protein

• Length: 383 a.a.
• Molecular weight: 42525.73 Da
• Isoelectric Point: 5.3036

>NTDB_id=83994 L6421_RS02510 WP_237262851.1 577973..579124(-) (pilU) [Thiomicrorhabdus immobilis strain Am19]
MSVTESLSELDKLLVYFSNNGGSDLFITAGRPATMKKDGKLISLTEDKLTAEMSRKLTFELMNEDQTEIFENTQECNFAY
HIAGIARYRVNVFVQRGTPGMVIRSVRTEIPNFESLNLPPILKESIMDKLGLILIVGGTGSGKSTTLASLLDVRNSTMDG
HIITIEDPIEFVHSHKKSIITQREVGVDTDSYENALKNTLRQAPNVILIGEIRSKETMEHAIAFSETGHLCLSTLHANNT
NQTIDRIVNFFPANQRDHILMDLSLNLKAIVSQRLIPKIGGGLIAAVEVLINTPRVAELIFKGKVSEIKQLMQSSGALGM
QTFDQALFELYENGLITYQDALRGADSMNDLRLNIKLNSKLPKPTDDEGELLNENVTFSLKQE

Nucleotide

• Length: 1152 bp

>NTDB_id=83994 L6421_RS02510 WP_237262851.1 577973..579124(-) (pilU) [Thiomicrorhabdus immobilis strain Am19]
ATGTCTGTCACAGAAAGCCTTTCCGAACTGGATAAACTTTTAGTCTATTTCTCTAACAATGGGGGGTCTGACCTTTTCAT
CACCGCAGGCCGACCAGCCACCATGAAAAAGGATGGCAAACTGATTTCATTAACCGAAGACAAACTCACGGCGGAGATGT
CGAGAAAGTTGACTTTTGAGTTGATGAATGAAGATCAAACGGAAATCTTTGAAAACACACAGGAATGTAACTTTGCCTAT
CACATTGCGGGGATAGCCCGTTACCGTGTTAACGTCTTTGTACAACGTGGAACCCCTGGCATGGTTATCCGTTCAGTTCG
TACTGAAATACCCAATTTTGAAAGTTTGAACCTTCCTCCGATTCTTAAAGAATCCATTATGGACAAACTCGGACTGATAC
TGATTGTTGGTGGAACGGGATCAGGTAAATCAACCACCTTGGCAAGCTTACTTGATGTACGAAACAGTACGATGGATGGT
CACATCATTACCATTGAAGACCCTATCGAATTTGTCCATAGCCATAAGAAAAGTATTATTACTCAGCGTGAAGTTGGGGT
GGATACCGATAGCTATGAAAATGCCTTGAAAAATACCTTACGACAAGCACCAAATGTCATATTGATTGGGGAAATCCGTA
GTAAAGAGACCATGGAGCATGCCATTGCGTTCTCGGAAACAGGTCACTTGTGCTTGTCTACTCTACACGCAAACAACACC
AACCAAACCATTGACCGTATTGTTAATTTCTTCCCTGCAAACCAACGCGACCACATTTTGATGGATTTATCTCTGAACCT
TAAAGCGATTGTCTCTCAGCGTCTGATTCCTAAAATAGGGGGTGGATTAATTGCCGCAGTAGAAGTGCTAATCAATACTC
CACGTGTCGCAGAGTTGATTTTTAAAGGCAAGGTCAGTGAGATAAAACAACTCATGCAGAGTTCTGGTGCCTTAGGCATG
CAAACATTTGACCAAGCTTTGTTTGAGCTATATGAAAATGGTCTCATTACCTACCAGGATGCACTTAGAGGCGCTGACTC
AATGAATGATTTACGCCTTAATATCAAATTAAATAGCAAACTACCGAAACCAACCGATGACGAAGGTGAGCTTTTAAATG
AAAACGTCACATTTAGTTTGAAACAAGAATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (121-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.64	92.167	0.54
pilU	Acinetobacter baylyi ADP1	53.476	97.65	0.522
pilU	Vibrio cholerae strain A1552	49.465	97.65	0.483
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.195	88.251	0.381
pilT	Legionella pneumophila strain Lp02	41.246	87.99	0.363
pilT	Legionella pneumophila strain ERS1305867	41.246	87.99	0.363