Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QPK06_RS06470 Genome accession   NZ_CP126578
Coordinates   1367271..1368305 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain AV066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1362271..1373305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPK06_RS06440 (QPK06_06440) glnK 1362859..1363197 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QPK06_RS06445 (QPK06_06445) arfB 1363399..1363815 (+) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QPK06_RS06450 (QPK06_06450) - 1363841..1364332 (+) 492 WP_124930248.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QPK06_RS06455 (QPK06_06455) - 1364692..1365117 (-) 426 WP_005340360.1 hypothetical protein -
  QPK06_RS06460 (QPK06_06460) yaaA 1365312..1366085 (-) 774 WP_064338818.1 peroxide stress protein YaaA -
  QPK06_RS06465 (QPK06_06465) pilU 1366133..1367242 (-) 1110 WP_124930305.1 type IVa pilus ATPase TapU Machinery gene
  QPK06_RS06470 (QPK06_06470) pilT 1367271..1368305 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QPK06_RS06475 (QPK06_06475) - 1368345..1369046 (+) 702 WP_013724131.1 YggS family pyridoxal phosphate-dependent enzyme -
  QPK06_RS06480 (QPK06_06480) proC 1369189..1370013 (+) 825 WP_139743295.1 pyrroline-5-carboxylate reductase -
  QPK06_RS06485 (QPK06_06485) - 1370025..1370576 (+) 552 WP_124930250.1 YggT family protein -
  QPK06_RS06490 (QPK06_06490) yggU 1370576..1370878 (+) 303 WP_110304033.1 DUF167 family protein YggU -
  QPK06_RS06495 (QPK06_06495) - 1370922..1371341 (+) 420 WP_113740088.1 DUF4426 domain-containing protein -
  QPK06_RS06500 (QPK06_06500) - 1371443..1372639 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=838919 QPK06_RS06470 WP_005340354.1 1367271..1368305(-) (pilT) [Aeromonas veronii strain AV066]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=838919 QPK06_RS06470 WP_005340354.1 1367271..1368305(-) (pilT) [Aeromonas veronii strain AV066]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCTGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACATCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACTCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATCCTTACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTGCACCGTGATACCAAGAGCTTCAGCAA
CGCCCTACGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATTGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGTTCCATGCTCTCCGAATCCCTGCGGGCAGTTATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGTGGCCGGGTTGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGTCTC
AAGCAGTTGGTGAGTCGTGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407