Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QPK06_RS06465 Genome accession   NZ_CP126578
Coordinates   1366133..1367242 (-) Length   369 a.a.
NCBI ID   WP_124930305.1    Uniprot ID   -
Organism   Aeromonas veronii strain AV066     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1361133..1372242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPK06_RS06435 (QPK06_06435) - 1361593..1362600 (-) 1008 WP_124930304.1 Fe(3+) ABC transporter substrate-binding protein -
  QPK06_RS06440 (QPK06_06440) glnK 1362859..1363197 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QPK06_RS06445 (QPK06_06445) arfB 1363399..1363815 (+) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QPK06_RS06450 (QPK06_06450) - 1363841..1364332 (+) 492 WP_124930248.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QPK06_RS06455 (QPK06_06455) - 1364692..1365117 (-) 426 WP_005340360.1 hypothetical protein -
  QPK06_RS06460 (QPK06_06460) yaaA 1365312..1366085 (-) 774 WP_064338818.1 peroxide stress protein YaaA -
  QPK06_RS06465 (QPK06_06465) pilU 1366133..1367242 (-) 1110 WP_124930305.1 type IVa pilus ATPase TapU Machinery gene
  QPK06_RS06470 (QPK06_06470) pilT 1367271..1368305 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QPK06_RS06475 (QPK06_06475) - 1368345..1369046 (+) 702 WP_013724131.1 YggS family pyridoxal phosphate-dependent enzyme -
  QPK06_RS06480 (QPK06_06480) proC 1369189..1370013 (+) 825 WP_139743295.1 pyrroline-5-carboxylate reductase -
  QPK06_RS06485 (QPK06_06485) - 1370025..1370576 (+) 552 WP_124930250.1 YggT family protein -
  QPK06_RS06490 (QPK06_06490) yggU 1370576..1370878 (+) 303 WP_110304033.1 DUF167 family protein YggU -
  QPK06_RS06495 (QPK06_06495) - 1370922..1371341 (+) 420 WP_113740088.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41080.11 Da        Isoelectric Point: 6.5659

>NTDB_id=838918 QPK06_RS06465 WP_124930305.1 1366133..1367242(-) (pilU) [Aeromonas veronii strain AV066]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIHRETLGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=838918 QPK06_RS06465 WP_124930305.1 1366133..1367242(-) (pilU) [Aeromonas veronii strain AV066]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGACCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGCGAAGCGCTTGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATTCACCGTGAAACCTTGGGCCGG
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATTTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCAGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCATATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTACCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACTCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGTTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGATTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36