Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P7644_RS02450 Genome accession   NZ_CP126238
Coordinates   512730..513773 (-) Length   347 a.a.
NCBI ID   WP_404301417.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain Ylb10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507730..518773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7644_RS02420 (P7644_02410) - 508143..508541 (+) 399 WP_404301411.1 YraN family protein -
  P7644_RS02425 (P7644_02415) - 508594..509196 (+) 603 WP_404301412.1 SIS domain-containing protein -
  P7644_RS02430 (P7644_02420) - 509193..509852 (+) 660 WP_404301413.1 BON domain-containing protein -
  P7644_RS02435 (P7644_02425) - 509856..510785 (-) 930 WP_404301414.1 NAD(P)-dependent oxidoreductase -
  P7644_RS02440 (P7644_02430) pilU 510867..512003 (-) 1137 WP_404301415.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P7644_RS02445 (P7644_02435) - 512028..512657 (-) 630 WP_404301416.1 cyclic nucleotide-binding domain-containing protein -
  P7644_RS02450 (P7644_02440) pilT 512730..513773 (-) 1044 WP_404301417.1 type IV pilus twitching motility protein PilT Machinery gene
  P7644_RS02455 (P7644_02445) - 513820..514545 (+) 726 WP_404301418.1 YggS family pyridoxal phosphate-dependent enzyme -
  P7644_RS02460 (P7644_02450) - 514590..515729 (+) 1140 WP_404301419.1 aminotransferase class V-fold PLP-dependent enzyme -
  P7644_RS02465 (P7644_02455) - 515736..516431 (-) 696 WP_404301420.1 MarC family protein -
  P7644_RS02470 (P7644_02460) - 516479..517963 (-) 1485 WP_404301421.1 FAD-linked oxidase C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37962.66 Da        Isoelectric Point: 6.8353

>NTDB_id=837226 P7644_RS02450 WP_404301417.1 512730..513773(-) (pilT) [Alicycliphilus denitrificans strain Ylb10]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMAYDIMSDAQRKAYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLGAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNESEYGHILTIED
PIEFVHESKKCLINQREVGPMTLSFSAALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKEMVRAMLSESLVAVVSQTLCKLKDGSGRVAAHEIMTGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=837226 P7644_RS02450 WP_404301417.1 512730..513773(-) (pilT) [Alicycliphilus denitrificans strain Ylb10]
GTGGACATCACCCAGTTGCTCGCCTTCAGCGTGAAGAACAAGGCGTCTGACCTGCATCTTTCCGCAGGCCTGCCGCCCAT
GATCCGGGTGCATGGCGATGTGCGGCGCATCAACGTCGACGCACTGGACCACAAGACCGTGCACGCCATGGCCTACGACA
TCATGAGCGATGCCCAGCGCAAGGCCTATGAAGAGTTTCTGGAGGTCGACTTTTCCTTCGAGATCGACGGCCTGGCACGC
TTTCGCGTGAATGCCTTCAACCAGAACCGCGGCGCAGCCGCCGTCTTTCGTACCATCCCGAGCAAGATCCTGACGCTGGA
GCAGTTGGGCGCCCCGAAGATCTTTGCCGACCTGGCGCTGAAACCCCGCGGCCTGGTGCTTGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTGAACCACCTGAACGAATCCGAGTACGGCCACATCCTGACCATTGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAATGCCTGATCAACCAGCGCGAGGTCGGGCCCATGACGCTGTCGTTCTCCGC
CGCCCTGCGCTCTGCCCTGCGCGAGGATCCGGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAGACCATACGCCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATAGACCGGGTG
ATCGACGTGTTTCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCCTGGTGGCCGTGGTCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGTCGGGTCGCGGCGCACGAGATCATGACCGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTCGGCATGCAGACCCTGGACCAGAAC
CTGACCGACCTGGTGCGGCGCAACATCATCAGCCCCGCCGAGGCGCGCAGCAAGGCCAAGATTCCGGACAACTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Neisseria meningitidis 8013

67.059

97.983

0.657
  pilT Neisseria gonorrhoeae MS11

66.765

97.983

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395