Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P7644_RS02440 Genome accession   NZ_CP126238
Coordinates   510867..512003 (-) Length   378 a.a.
NCBI ID   WP_404301415.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain Ylb10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505867..517003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7644_RS02410 (P7644_02400) - 505962..506972 (-) 1011 WP_404301409.1 NAD-dependent epimerase/dehydratase family protein -
  P7644_RS02415 (P7644_02405) rsmI 507220..508143 (-) 924 WP_404301410.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  P7644_RS02420 (P7644_02410) - 508143..508541 (+) 399 WP_404301411.1 YraN family protein -
  P7644_RS02425 (P7644_02415) - 508594..509196 (+) 603 WP_404301412.1 SIS domain-containing protein -
  P7644_RS02430 (P7644_02420) - 509193..509852 (+) 660 WP_404301413.1 BON domain-containing protein -
  P7644_RS02435 (P7644_02425) - 509856..510785 (-) 930 WP_404301414.1 NAD(P)-dependent oxidoreductase -
  P7644_RS02440 (P7644_02430) pilU 510867..512003 (-) 1137 WP_404301415.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P7644_RS02445 (P7644_02435) - 512028..512657 (-) 630 WP_404301416.1 cyclic nucleotide-binding domain-containing protein -
  P7644_RS02450 (P7644_02440) pilT 512730..513773 (-) 1044 WP_404301417.1 type IV pilus twitching motility protein PilT Machinery gene
  P7644_RS02455 (P7644_02445) - 513820..514545 (+) 726 WP_404301418.1 YggS family pyridoxal phosphate-dependent enzyme -
  P7644_RS02460 (P7644_02450) - 514590..515729 (+) 1140 WP_404301419.1 aminotransferase class V-fold PLP-dependent enzyme -
  P7644_RS02465 (P7644_02455) - 515736..516431 (-) 696 WP_404301420.1 MarC family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41690.17 Da        Isoelectric Point: 7.1529

>NTDB_id=837225 P7644_RS02440 WP_404301415.1 510867..512003(-) (pilU) [Alicycliphilus denitrificans strain Ylb10]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARSIMSDRQIADFERSKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEIAMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWGAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIVNFFPEERRAQLLMDLSLNLKAMVSQRLLPKQDGKGRAAAVEVMLNTPLIADLIFKGEVVEIKEIMKKSRNLG
MQTFDQSLFDLFEANVIGYEDALRNADSLNDLRLQIKLGSQRTKTTDLSSGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=837225 P7644_RS02440 WP_404301415.1 510867..512003(-) (pilU) [Alicycliphilus denitrificans strain Ylb10]
ATGGAACGCGACCAGGCCAGCAAATTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCGGCGGCAGCGATCTGTTCAT
CACGGCCGAGTTTCCGCCGGCGATCAAGGTCGATGGCAAGGTCACCAAGGTCTCGCCCCAGCCACTGACACCGGCGCACA
CGCTGACGCTGGCGCGCTCCATCATGAGCGATCGCCAGATCGCGGACTTCGAACGCTCCAAGGAGTGCAATTTCGCCATC
TCGCCCGCCGGCATTGGGCGCTTTCGCGTGAACGCCTTCATCCAGCAGGGCCGCGTAGGCATGGTGCTGCGCACGATTCC
GCTCACCCTGCCCACCATCGATGGTCTGGGCGTGCCGCAGGTGCTCAAGGAAATCGCCATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCAACCACGCTGGCGGCCATGGTGGACTGGCGCAACGAGAATTCCTTCGGC
CACATCATCACCGTGGAAGACCCAATCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTCACCCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGGGGCGGCCCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGCCGTGCGCAGCTGCTCATGGACCTGTCGCTGAACCT
CAAGGCCATGGTGTCGCAGCGCCTGCTGCCCAAGCAGGACGGCAAGGGCCGGGCCGCGGCCGTCGAGGTCATGCTCAACA
CCCCCCTGATCGCCGACCTGATCTTCAAGGGTGAGGTCGTCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTTGACCTGTTCGAGGCCAATGTCATCGGCTACGAGGACGCGCTGCGCAACGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCGGCAGCCAGCGCACCAAGACCACCGACCTGTCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.043

92.328

0.601
  pilU Acinetobacter baylyi ADP1

56.983

94.709

0.54
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.291

89.153

0.413
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Acinetobacter nosocomialis M2

44.745

88.095

0.394
  pilT Legionella pneumophila strain Lp02

44.745

88.095

0.394
  pilT Legionella pneumophila strain ERS1305867

44.745

88.095

0.394
  pilT Acinetobacter baumannii D1279779

44.745

88.095

0.394
  pilT Acinetobacter baumannii strain A118

44.745

88.095

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37