Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OH686_RS10735 Genome accession   NZ_CP126126
Coordinates   2261400..2262434 (-) Length   344 a.a.
NCBI ID   WP_289538480.1    Uniprot ID   -
Organism   Pseudomonas sp. SO81     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2256400..2267434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH686_RS10715 (OH686_10950) - 2257030..2257428 (-) 399 WP_289538474.1 TM2 domain-containing protein -
  OH686_RS10720 (OH686_10955) - 2257536..2258747 (+) 1212 WP_289538476.1 putative DNA modification/repair radical SAM protein -
  OH686_RS10725 (OH686_10960) - 2258747..2260195 (+) 1449 WP_289538478.1 UdgX family uracil-DNA binding protein -
  OH686_RS10730 (OH686_10965) pilU 2260196..2261341 (-) 1146 WP_192399133.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OH686_RS10735 (OH686_10970) pilT 2261400..2262434 (-) 1035 WP_289538480.1 type IV pilus twitching motility protein PilT Machinery gene
  OH686_RS10740 (OH686_10975) - 2262507..2263199 (+) 693 WP_289538482.1 YggS family pyridoxal phosphate-dependent enzyme -
  OH686_RS10745 (OH686_10980) proC 2263213..2264031 (+) 819 WP_289538484.1 pyrroline-5-carboxylate reductase -
  OH686_RS10750 (OH686_10985) - 2264041..2264634 (+) 594 WP_289538486.1 YggT family protein -
  OH686_RS10755 (OH686_10990) - 2264636..2264932 (+) 297 WP_289538488.1 DUF167 family protein -
  OH686_RS10760 (OH686_10995) - 2265075..2267063 (+) 1989 WP_289538490.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37926.60 Da        Isoelectric Point: 6.8889

>NTDB_id=836763 OH686_RS10735 WP_289538480.1 2261400..2262434(-) (pilT) [Pseudomonas sp. SO81]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGALGMQTLDNCL
KGLVSKGLVSREAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=836763 OH686_RS10735 WP_289538480.1 2261400..2262434(-) (pilT) [Pseudomonas sp. SO81]
ATGGATATTACCGAGCTGCTTGCCTTCAGTGCCAAGCAAGGCGCGTCGGACCTGCACCTCTCGGCGGGTCTGCCGCCGAT
GATTCGTGTCGATGGCGACGTACGGCGGATCAACCTGCCGGCGATGGACCACAAACAGGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAGACCGACTTCTCCTTCGAAGTGCCTGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTCTTGAGCATGGA
GGACCTGGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGTCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTGAACAGCAACAAGTACCACCACATCCTCACCATCGAAGAC
CCGATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGTGATACCCACGGTTTCTCCGA
AGCCCTGCGTTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTCCGCTCCATGCTGTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGCGTGGCGGCCCACGAGATCATGATCGGTACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGTGGTGCGCTGGGCATGCAGACCCTCGACAACTGCCTC
AAGGGCTTGGTTTCCAAGGGCCTGGTCAGCCGCGAGGCGGCCCGCGAGAAGGCCAAGACACCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Acinetobacter nosocomialis M2

85.174

100

0.852
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395