Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QNH36_RS17645 Genome accession   NZ_CP126080
Coordinates   3481735..3482775 (-) Length   346 a.a.
NCBI ID   WP_283903891.1    Uniprot ID   A0AA95HVA6
Organism   Mesobacillus sp. AQ2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3476735..3487775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH36_RS17620 (QNH36_17620) - 3477529..3478170 (-) 642 WP_283903889.1 hypothetical protein -
  QNH36_RS17625 (QNH36_17625) pilM 3478167..3479162 (-) 996 WP_283903890.1 pilus assembly protein PilM -
  QNH36_RS17630 (QNH36_17630) comC 3479179..3479931 (-) 753 WP_283905433.1 A24 family peptidase Machinery gene
  QNH36_RS17635 (QNH36_17635) - 3479936..3480400 (-) 465 WP_144477703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QNH36_RS17640 (QNH36_17640) - 3480527..3481732 (-) 1206 WP_144477701.1 type II secretion system F family protein -
  QNH36_RS17645 (QNH36_17645) pilT 3481735..3482775 (-) 1041 WP_283903891.1 type IV pilus twitching motility protein PilT Machinery gene
  QNH36_RS17650 (QNH36_17650) - 3482789..3484450 (-) 1662 WP_283903892.1 ATPase, T2SS/T4P/T4SS family -
  QNH36_RS17655 (QNH36_17655) - 3484476..3485828 (-) 1353 WP_283903893.1 VanW family protein -
  QNH36_RS17660 (QNH36_17660) - 3486160..3486576 (+) 417 WP_283903894.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QNH36_RS17665 (QNH36_17665) - 3486581..3487009 (+) 429 WP_283903895.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38004.95 Da        Isoelectric Point: 7.6762

>NTDB_id=834713 QNH36_RS17645 WP_283903891.1 3481735..3482775(-) (pilT) [Mesobacillus sp. AQ2]
MKEKIEHLLSTGFERKASDIHITVGVPPILRINGELQRFGTEAILPEDTEGMAKAIIPAKLWDDFKSKGELDFSYGIPGL
SRFRVNAYHQRGGVSLAARVVPTTIPTLEELELPSVLKKIAEKPQGLVLVTGPTGSGKSTTLAAMIQYINKNMSKHIVTL
EDPIEYLHKHGNSIIDQREVGFDTNNFANGLRAALRQDPDVILVGEMRDLDTIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPSAQQAQIRIQLASVLVAVVSQRLFPTADKRSRRGATEILINNAAVANLIRNEKIHQIISIMQTSKAFGMHTLE
MSIKELVMQGIISRESAEPYLLEKLV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=834713 QNH36_RS17645 WP_283903891.1 3481735..3482775(-) (pilT) [Mesobacillus sp. AQ2]
ATGAAAGAAAAAATCGAACATTTGCTGAGTACCGGATTTGAACGTAAGGCCTCTGACATCCACATTACAGTCGGTGTGCC
ACCCATATTGAGAATCAACGGTGAATTACAAAGATTTGGCACAGAGGCGATTTTGCCTGAAGATACAGAGGGAATGGCAA
AAGCAATCATTCCTGCAAAACTATGGGACGACTTCAAGTCCAAGGGGGAACTGGATTTCTCCTACGGCATACCTGGCTTA
TCGAGATTCAGGGTCAATGCTTATCACCAGAGGGGAGGTGTCTCCCTCGCAGCACGTGTTGTCCCAACCACAATTCCTAC
TCTTGAGGAACTGGAACTCCCCTCGGTCTTGAAGAAGATTGCCGAGAAGCCACAGGGACTTGTACTCGTTACCGGGCCAA
CAGGAAGCGGGAAGTCAACAACACTGGCAGCAATGATACAGTACATAAATAAAAATATGTCGAAGCACATAGTCACACTA
GAGGACCCAATTGAGTATTTGCATAAGCATGGGAACAGCATCATAGACCAGCGTGAAGTGGGCTTCGATACTAACAACTT
TGCCAACGGATTGCGTGCCGCGCTCAGACAGGATCCTGACGTAATCCTGGTAGGGGAAATGCGTGACCTTGATACAATCC
AGACGGCTATTACTGCTGCTGAAACAGGCCATCTCGTGCTGGGTACATTGCATACATCAAGTGCTCCGGCAACCATCAAC
CGGATTATCGATGTGTTTCCATCCGCCCAGCAAGCGCAAATCCGGATCCAGCTGGCTTCAGTCCTGGTTGCTGTTGTTTC
ACAGCGATTGTTTCCGACGGCTGATAAAAGAAGCCGCCGCGGAGCAACTGAAATCCTGATCAATAATGCGGCAGTGGCCA
ATTTGATCCGGAATGAAAAAATCCACCAAATCATCAGCATTATGCAAACATCAAAAGCATTTGGCATGCACACGCTTGAG
ATGAGCATTAAGGAACTGGTCATGCAGGGGATTATCTCGAGGGAATCAGCTGAACCATACTTACTGGAGAAGTTGGTGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.057

95.665

0.488
  pilT Neisseria meningitidis 8013

49.701

96.532

0.48
  pilT Neisseria gonorrhoeae MS11

49.401

96.532

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

96.532

0.471
  pilT Vibrio cholerae strain A1552

48.802

96.532

0.471
  pilT Legionella pneumophila strain Lp02

47.353

98.266

0.465
  pilT Legionella pneumophila strain ERS1305867

47.353

98.266

0.465
  pilT Acinetobacter nosocomialis M2

48.348

96.243

0.465
  pilT Acinetobacter baylyi ADP1

48.348

96.243

0.465
  pilT Acinetobacter baumannii D1279779

48.348

96.243

0.465
  pilT Acinetobacter baumannii strain A118

48.348

96.243

0.465
  pilT Pseudomonas aeruginosa PAK

47.447

96.243

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.006

96.532

0.454
  pilU Pseudomonas stutzeri DSM 10701

43.413

96.532

0.419
  pilU Acinetobacter baylyi ADP1

40.483

95.665

0.387
  pilU Vibrio cholerae strain A1552

41.304

93.064

0.384