Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JLC71_RS09920 Genome accession   NZ_AP024094
Coordinates   2081693..2082736 (-) Length   347 a.a.
NCBI ID   WP_200915265.1    Uniprot ID   A0A7I8EAQ2
Organism   Jeongeupia sp. HS-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2076693..2087736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLC71_RS09910 (JHS3_19270) - 2078377..2080413 (-) 2037 WP_200915264.1 FimV family protein -
  JLC71_RS09915 (JHS3_19280) pilU 2080485..2081621 (-) 1137 WP_305066867.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JLC71_RS09920 (JHS3_19290) pilT 2081693..2082736 (-) 1044 WP_200915265.1 type IV pilus twitching motility protein PilT Machinery gene
  JLC71_RS09925 (JHS3_19300) - 2082829..2083515 (+) 687 WP_236251041.1 YggS family pyridoxal phosphate-dependent enzyme -
  JLC71_RS09930 (JHS3_19310) proC 2083577..2084392 (+) 816 WP_200915267.1 pyrroline-5-carboxylate reductase -
  JLC71_RS09935 (JHS3_19320) - 2084402..2084989 (+) 588 WP_200915268.1 YggT family protein -
  JLC71_RS09940 (JHS3_19330) - 2084995..2085294 (+) 300 WP_200915269.1 DUF167 domain-containing protein -
  JLC71_RS09945 (JHS3_19340) - 2085315..2085734 (-) 420 WP_200915270.1 FxsA family protein -
  JLC71_RS09950 (JHS3_19350) cutA 2085733..2086080 (+) 348 WP_200915271.1 divalent-cation tolerance protein CutA -
  JLC71_RS09955 (JHS3_19360) dsbD 2086080..2087642 (+) 1563 WP_236251042.1 protein-disulfide reductase DsbD -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38471.27 Da        Isoelectric Point: 6.8841

>NTDB_id=83462 JLC71_RS09920 WP_200915265.1 2081693..2082736(-) (pilT) [Jeongeupia sp. HS-3]
MEISELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMDHKDVHDMIYDIMNDGQRKVYEDTLECDFSFEIPNLAR
FRVNAFVQQRGAGAVFRTIPSKVLTLEELNAPKIFTEIAQNPRGLVLVTGPTGSGKSTTLAAILNYVNENEYGHILTVED
PIEFVHQSKKCLINQREVGPHTMSFSAALRSALREDPDVILIGEMRDLETIRLALTAAETGHLVFGTLHTSTAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGIPAIRNLIRENKIAQMYSSIQTGQQYGMQTLDQC
LQGLVQRNIVSMAEARTKAASPDHFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=83462 JLC71_RS09920 WP_200915265.1 2081693..2082736(-) (pilT) [Jeongeupia sp. HS-3]
ATGGAAATTTCTGAACTCTTGGCCTTTGCCGTCAAGAACAAGGCATCCGACTTGCACCTGTCGTCCGGCCTGCCGCCGAT
GATTCGCGTGCATGGCGACGTGCGGCGCATCAACCTGCCGCCGATGGATCACAAAGATGTTCATGACATGATCTATGACA
TCATGAACGACGGTCAGCGCAAGGTCTATGAAGACACGCTCGAGTGCGATTTCTCGTTCGAGATCCCCAATCTGGCGCGT
TTCCGGGTCAACGCCTTCGTGCAGCAGCGCGGTGCCGGTGCGGTCTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
AGAGCTTAACGCACCGAAGATCTTTACCGAAATCGCCCAGAACCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACGACGCTGGCGGCGATTCTCAATTACGTCAACGAGAACGAATACGGCCATATCCTGACCGTTGAAGAT
CCGATCGAGTTCGTACACCAATCGAAAAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGCATACGATGAGCTTTTCCGC
TGCGCTGCGCAGTGCATTGCGGGAAGATCCGGATGTCATCCTCATCGGCGAAATGCGTGATCTGGAAACGATCCGGCTGG
CGCTGACCGCGGCTGAAACCGGCCACCTGGTCTTCGGTACGCTGCACACCAGTACCGCGGCCAAGACCATCGACCGGATT
GTCGATGTGTTTCCGGCTGCCGAAAAGGAGATGGTCCGGTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATTTCGCAGAC
GCTGCTGAAAACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAAATCATGATCGGCATTCCGGCGATTCGTAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGTCGATTCAGACCGGCCAGCAGTACGGTATGCAGACGCTGGATCAATGT
CTGCAGGGTCTGGTTCAGCGTAATATCGTGTCGATGGCCGAAGCACGAACCAAGGCCGCCAGCCCGGATCACTTCAAGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7I8EAQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria meningitidis 8013

72.566

97.695

0.709
  pilT Neisseria gonorrhoeae MS11

72.271

97.695

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

41.329

99.712

0.412
  pilU Acinetobacter baylyi ADP1

40.346

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.462

99.712

0.403

Multiple sequence alignment