Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JLC71_RS09915 Genome accession   NZ_AP024094
Coordinates   2080485..2081621 (-) Length   378 a.a.
NCBI ID   WP_305066867.1    Uniprot ID   A0A7I8EB31
Organism   Jeongeupia sp. HS-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2075485..2086621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLC71_RS09910 (JHS3_19270) - 2078377..2080413 (-) 2037 WP_200915264.1 FimV family protein -
  JLC71_RS09915 (JHS3_19280) pilU 2080485..2081621 (-) 1137 WP_305066867.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JLC71_RS09920 (JHS3_19290) pilT 2081693..2082736 (-) 1044 WP_200915265.1 type IV pilus twitching motility protein PilT Machinery gene
  JLC71_RS09925 (JHS3_19300) - 2082829..2083515 (+) 687 WP_236251041.1 YggS family pyridoxal phosphate-dependent enzyme -
  JLC71_RS09930 (JHS3_19310) proC 2083577..2084392 (+) 816 WP_200915267.1 pyrroline-5-carboxylate reductase -
  JLC71_RS09935 (JHS3_19320) - 2084402..2084989 (+) 588 WP_200915268.1 YggT family protein -
  JLC71_RS09940 (JHS3_19330) - 2084995..2085294 (+) 300 WP_200915269.1 DUF167 domain-containing protein -
  JLC71_RS09945 (JHS3_19340) - 2085315..2085734 (-) 420 WP_200915270.1 FxsA family protein -
  JLC71_RS09950 (JHS3_19350) cutA 2085733..2086080 (+) 348 WP_200915271.1 divalent-cation tolerance protein CutA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42339.57 Da        Isoelectric Point: 6.5692

>NTDB_id=83461 JLC71_RS09915 WP_305066867.1 2080485..2081621(-) (pilU) [Jeongeupia sp. HS-3]
MEKEQSAKFMHDLLRHMRTKDASDLFITADFPPAMKIDGRVTPVSNQMLSAQHTKELARAIMNDRQAEEFEGSKECNFAI
SPGNLGRFRVNAFMQQGRVGMVLRTINSEIPKLEELGLPSVLKDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNETAYD
HIITIEDPIEYVHEHKRSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSA
NQALDRIINFFPEERKAQLLMDLSLNLKAFVSQRLVPHRAGKGRVAAVEVMLNSPLISELVFKGDVHEIKEIMKRSRELG
MQTFDQSLFDLHEAGKISYEDALRNADSVNDLRLQIKLNGSDAKQKDVLSGFDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=83461 JLC71_RS09915 WP_305066867.1 2080485..2081621(-) (pilU) [Jeongeupia sp. HS-3]
ATGGAGAAAGAACAGTCCGCGAAATTCATGCATGACTTGCTGCGGCACATGCGAACCAAGGATGCGTCCGATCTTTTCAT
TACCGCCGATTTTCCGCCGGCGATGAAGATCGATGGCCGGGTCACGCCGGTGTCGAACCAGATGCTCTCGGCCCAGCACA
CCAAGGAGCTGGCGCGCGCGATCATGAATGATCGGCAGGCCGAGGAGTTCGAAGGCAGCAAGGAGTGCAATTTCGCCATC
AGCCCGGGCAACCTGGGGCGTTTTCGCGTCAATGCCTTCATGCAGCAAGGCCGTGTCGGCATGGTGCTGCGGACGATCAA
TTCCGAAATCCCCAAACTCGAAGAGCTGGGCCTGCCGTCGGTATTGAAAGACATCGCCATGACCAAGCGCGGGCTGGTGA
TCTTTGTCGGCGGTACCGGCAGCGGCAAGTCGACCTCGCTGGCGGCAATGATCGGGCATCGCAACGAGACGGCCTACGAT
CACATCATCACGATTGAAGACCCGATCGAGTATGTGCACGAGCACAAGCGCTCGATCATCACCCAGCGCGAAGTCGGCGT
GGATACCGATTCATGGATGGCTGCGCTGAAGAATACGTTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ATCGTGAAACCATGGACTACGCGATCGCCTTCGCCGAAACCGGCCATCTGTGCATGGCGACGCTGCATGCCAACTCGGCC
AATCAGGCGTTGGATCGGATCATCAACTTTTTCCCGGAAGAACGTAAGGCACAGTTGCTGATGGATTTGTCGCTGAATCT
CAAGGCTTTCGTGTCGCAGCGTCTGGTGCCGCATCGTGCGGGCAAGGGCCGTGTGGCTGCGGTTGAGGTGATGCTCAACA
GCCCGCTGATTTCCGAGCTGGTGTTCAAGGGCGACGTGCACGAGATCAAGGAAATCATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGAGCCTGTTCGATCTCCATGAGGCGGGCAAAATCAGTTACGAGGATGCACTGCGCAATGCCGA
TTCGGTCAATGACCTGCGCTTGCAGATCAAGCTCAACGGGAGTGACGCGAAGCAGAAAGACGTGCTGAGCGGATTCGACC
ACCTGGATATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(83-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7I8EB31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.873

91.534

0.585
  pilU Acinetobacter baylyi ADP1

58.774

94.974

0.558
  pilU Vibrio cholerae strain A1552

54.674

93.386

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.866

88.624

0.415
  pilT Pseudomonas aeruginosa PAK

43.582

88.624

0.386
  pilT Legionella pneumophila strain Lp02

43.807

87.566

0.384
  pilT Legionella pneumophila strain ERS1305867

43.807

87.566

0.384
  pilT Acinetobacter baylyi ADP1

45.171

84.921

0.384
  pilT Acinetobacter baumannii D1279779

44.548

84.921

0.378
  pilT Acinetobacter nosocomialis M2

44.548

84.921

0.378
  pilT Acinetobacter baumannii strain A118

44.548

84.921

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.624

0.376
  pilT Neisseria gonorrhoeae MS11

41.246

89.153

0.368
  pilT Neisseria meningitidis 8013

41.246

89.153

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.987

83.598

0.368
  pilT Vibrio cholerae strain A1552

43.987

83.598

0.368

Multiple sequence alignment