Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMY55_RS21745 Genome accession   NZ_CP125947
Coordinates   4684775..4685818 (+) Length   347 a.a.
NCBI ID   WP_283486187.1    Uniprot ID   -
Organism   Comamonas resistens strain ZM22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4679775..4690818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY55_RS21725 (QMY55_21725) - 4680052..4681650 (-) 1599 WP_283486183.1 AMP-binding protein -
  QMY55_RS21730 (QMY55_21730) - 4681816..4682571 (+) 756 WP_283486184.1 TetR/AcrR family transcriptional regulator -
  QMY55_RS21735 (QMY55_21735) - 4682614..4683861 (+) 1248 WP_283486185.1 branched-chain amino acid ABC transporter substrate-binding protein -
  QMY55_RS21740 (QMY55_21740) - 4684006..4684737 (-) 732 WP_283486186.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMY55_RS21745 (QMY55_21745) pilT 4684775..4685818 (+) 1044 WP_283486187.1 type IV pilus twitching motility protein PilT Machinery gene
  QMY55_RS21750 (QMY55_21750) pilU 4685946..4687082 (+) 1137 WP_283486188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMY55_RS21755 (QMY55_21755) - 4687204..4688109 (+) 906 WP_283486189.1 NAD(P)-dependent oxidoreductase -
  QMY55_RS21760 (QMY55_21760) - 4688264..4688917 (-) 654 WP_283486190.1 BON domain-containing protein -
  QMY55_RS21765 (QMY55_21765) - 4688914..4689522 (-) 609 WP_283486191.1 SIS domain-containing protein -
  QMY55_RS21770 (QMY55_21770) - 4689601..4689990 (-) 390 WP_283486192.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38330.18 Da        Isoelectric Point: 6.7743

>NTDB_id=833645 QMY55_RS21745 WP_283486187.1 4684775..4685818(+) (pilT) [Comamonas resistens strain ZM22]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFSDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNMGMQTLDQN
LTDLVRRNVISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=833645 QMY55_RS21745 WP_283486187.1 4684775..4685818(+) (pilT) [Comamonas resistens strain ZM22]
GTGGACATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCCGCGGGACTGCCACCCAT
GATCCGCGTCCACGGCGATGTGCGCCGCATCAATATCGATCCGCTCGATCACAAGACCGTGCACGCCATGGTCTACGACA
TCATGAGCGATGCGCAGCGCAAGCAGTACGAAGAATTCCTGGAGGTGGACTTCTCGTTCGAGATCGAAGGTCTGGCCCGC
TTCCGTGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGTACCATTCCGAGCAAGATTCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATTTTCTCCGACCTGGCCCTGAAGCCGCGCGGACTGGTGCTCGTAACCGGCCCCACGGGTT
CGGGCAAATCGACCACGCTGGCAGCCATGGTCAACTACCTCAACGAGACCGAGTACGGCCACATTCTGACGGTGGAAGAC
CCCATCGAATTCGTGCATGAATCCAAGAAATGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCCGC
GGCGCTGAAGTCCGCGCTGCGTGAGGACCCGGACGCGATTCTGGTCGGTGAAATGCGCGACCTGGAAACCATACGCCTGG
CCATGACGGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCATCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTTGCGGCGCACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TTCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCCAGAACATGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTTCGCCGCAATGTCATCAGCGCCGCCGAGGCCCGTTCCAAGGCGAAAATTCCCGAAAATTTCCCTGG
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409