Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QMY55_RS21750 Genome accession   NZ_CP125947
Coordinates   4685946..4687082 (+) Length   378 a.a.
NCBI ID   WP_283486188.1    Uniprot ID   -
Organism   Comamonas resistens strain ZM22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4680946..4692082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY55_RS21730 (QMY55_21730) - 4681816..4682571 (+) 756 WP_283486184.1 TetR/AcrR family transcriptional regulator -
  QMY55_RS21735 (QMY55_21735) - 4682614..4683861 (+) 1248 WP_283486185.1 branched-chain amino acid ABC transporter substrate-binding protein -
  QMY55_RS21740 (QMY55_21740) - 4684006..4684737 (-) 732 WP_283486186.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMY55_RS21745 (QMY55_21745) pilT 4684775..4685818 (+) 1044 WP_283486187.1 type IV pilus twitching motility protein PilT Machinery gene
  QMY55_RS21750 (QMY55_21750) pilU 4685946..4687082 (+) 1137 WP_283486188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMY55_RS21755 (QMY55_21755) - 4687204..4688109 (+) 906 WP_283486189.1 NAD(P)-dependent oxidoreductase -
  QMY55_RS21760 (QMY55_21760) - 4688264..4688917 (-) 654 WP_283486190.1 BON domain-containing protein -
  QMY55_RS21765 (QMY55_21765) - 4688914..4689522 (-) 609 WP_283486191.1 SIS domain-containing protein -
  QMY55_RS21770 (QMY55_21770) - 4689601..4689990 (-) 390 WP_283486192.1 YraN family protein -
  QMY55_RS21775 (QMY55_21775) rsmI 4689990..4690913 (+) 924 WP_283486193.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41888.33 Da        Isoelectric Point: 6.7686

>NTDB_id=833646 QMY55_RS21750 WP_283486188.1 4685946..4687082(+) (pilU) [Comamonas resistens strain ZM22]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSPLHTLALARSIMSDKQVADFEHTKECNFAI
SPAGVGRFRVNAFVQQGKVGMVLRTIPTTLPTIDGLGVPQILKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIVSQRLIPKQDAKGRVAAIEVMLNSPLIADLIFKGEVAEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTQDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=833646 QMY55_RS21750 WP_283486188.1 4685946..4687082(+) (pilU) [Comamonas resistens strain ZM22]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGAGTTTCCGCCAGCCATCAAGATCGATGGCAAGGTGACCAAGGTCTCGCCCCAGCCGCTGTCGCCGTTGCACA
CGCTGGCTCTGGCCCGTTCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCTCCGGCCGGCGTGGGGCGTTTTCGCGTCAATGCCTTTGTGCAGCAGGGCAAGGTCGGCATGGTTCTGCGGACCATCCC
CACCACGCTGCCCACGATCGACGGCCTGGGTGTGCCCCAGATCCTCAAGGAAGTGACCATGACCAAGCGTGGTCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGTAAATCGACCACTCTGGCCGCCATGGTGGACTGGCGCAACGAGAATTCCTTCGGC
CACATCATCACCGTGGAAGACCCCGTGGAATTCGTGCATCCGCACAAGAACTGCGTGGTCACGCAACGCGAGGTGGGTCT
GGATACGGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATTCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCGATTGCCTTCTCCGAAACCGGGCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGCCGCCCCCAGCTGCTGATGGATGTGTCGCTGAATCT
GCGCGCCATCGTCTCCCAGCGCCTGATTCCCAAGCAGGACGCCAAGGGCCGCGTGGCCGCCATCGAGGTGATGCTGAACT
CCCCCCTGATTGCCGATCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACATCGGT
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTCTTCGAGTCCAATCTCATCACCTATGAGGATGCGCTGCGCAATGCCGA
CTCGGTCAATGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGCACCCAGGATCTGGCTGCTGGTACCGAGC
ATCTGACGATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.475

96.825

0.595
  pilU Acinetobacter baylyi ADP1

56.941

93.386

0.532
  pilU Vibrio cholerae strain A1552

52.066

96.032

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Acinetobacter baylyi ADP1

42.073

86.772

0.365
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362