Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QLH32_RS15560 Genome accession   NZ_CP125669
Coordinates   3265990..3267027 (-) Length   345 a.a.
NCBI ID   WP_267980244.1    Uniprot ID   -
Organism   Acinetobacter sp. KCTC 92772     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3260990..3272027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLH32_RS15530 (QLH32_15530) - 3261633..3262088 (+) 456 WP_267980240.1 bacteriohemerythrin -
  QLH32_RS15535 (QLH32_15535) - 3262338..3263420 (+) 1083 WP_283266949.1 hypothetical protein -
  QLH32_RS15540 (QLH32_15540) - 3263420..3263743 (+) 324 WP_283266950.1 RnfH family protein -
  QLH32_RS15545 (QLH32_15545) bamE 3263797..3264195 (-) 399 WP_004676608.1 outer membrane protein assembly factor BamE -
  QLH32_RS15550 (QLH32_15550) fur 3264307..3264744 (+) 438 WP_004682786.1 ferric iron uptake transcriptional regulator -
  QLH32_RS15555 (QLH32_15555) pilU 3264846..3265964 (-) 1119 WP_279959583.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QLH32_RS15560 (QLH32_15560) pilT 3265990..3267027 (-) 1038 WP_267980244.1 type IV pilus twitching motility protein PilT Machinery gene
  QLH32_RS15565 (QLH32_15565) - 3267154..3267840 (+) 687 WP_283266951.1 YggS family pyridoxal phosphate-dependent enzyme -
  QLH32_RS15570 (QLH32_15570) - 3267837..3268628 (-) 792 WP_283266952.1 transglutaminase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38389.01 Da        Isoelectric Point: 6.6655

>NTDB_id=832364 QLH32_RS15560 WP_267980244.1 3265990..3267027(-) (pilT) [Acinetobacter sp. KCTC 92772]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGIINTQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=832364 QLH32_RS15560 WP_267980244.1 3265990..3267027(-) (pilT) [Acinetobacter sp. KCTC 92772]
ATGGATATTACAGAACTACTCGCCTTTTCAGTCAAAAACGGTGCTTCGGATTTACACCTTTCAGCGGGTATGCCTCCTAT
GATTCGTGTGGATGGTGAAGTTCGCCGAATCAACTTGCCAGCACTTGAGCATAAAGATGTACACCGTCTGGTGTATGACA
TCATGAACGATAAACAACGTCGTGACTATGAAGAAAAGCTGGAAACAGACTTTTCATTTGAAGTTCCCAACATTGCCCGT
TTCCGTGTGAACGCATTTAACCAGAACCGTGGTGCTGGTGCGGTGTTCCGTACCATTCCATCAAAGGTTTTGACCATGGA
AGACCTTGGCCTTGGTTCAATCTTCAAGGAAATCTGTGATTATCCACGCGGGATTGTACTGGTAACAGGACCGACAGGTT
CTGGTAAGTCAACCACACTTGCTGCGATGATCGACTATATTAACGAAAACCGTTATGACCACATTTTAACGGTCGAAGAC
CCAATCGAATTTGTACACCAGTCTAAAAAATGCCTGATCAACCAGCGTGAAGTGCACCGTGATACACATGGCTTCAATGA
AGCACTGCGTTCAGCACTGCGTGAAGACCCTGACATTATCCTGGTTGGTGAGATGCGTGACCTGGAAACCATTCGTCTGG
CATTGACTGCTGCGGAAACGGGTCACTTGGTGTTTGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTGCGTGCCATGTTGTCAGAATCATTACAAGCCGTAATTTCACAAAC
ACTCTTGAAAAAGAATGGTGGCGGTCGTGTTGCAGCGCACGAGATCATGATTGGTATTCCTGCAATTCGTAACCTTGTCC
GTGAGAACAAGGTTGCCCAAATGTACTCTGCAATTCAGACAGGTGCAAACTATGGTATGACCACACTGGATCAAAGTCTG
AAAACACTTGTATCCAAGGGTATTATCAATACTCAAACTGCCCGTACCGCAGCAAAACAGCCAGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.812

100

0.968
  pilT Acinetobacter baumannii D1279779

96.522

100

0.965
  pilT Acinetobacter baumannii strain A118

96.522

100

0.965
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

76.163

99.71

0.759
  pilT Legionella pneumophila strain ERS1305867

76.163

99.71

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.94

97.391

0.681
  pilT Vibrio cholerae strain A1552

69.94

97.391

0.681
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388