Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QLH32_RS15555 Genome accession   NZ_CP125669
Coordinates   3264846..3265964 (-) Length   372 a.a.
NCBI ID   WP_279959583.1    Uniprot ID   -
Organism   Acinetobacter sp. KCTC 92772     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3259846..3270964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLH32_RS15520 (QLH32_15520) - 3260116..3260964 (+) 849 WP_283266946.1 class II glutamine amidotransferase -
  QLH32_RS15525 (QLH32_15525) - 3260967..3261383 (+) 417 WP_283266947.1 zinc ABC transporter substrate-binding protein -
  QLH32_RS15530 (QLH32_15530) - 3261633..3262088 (+) 456 WP_267980240.1 bacteriohemerythrin -
  QLH32_RS15535 (QLH32_15535) - 3262338..3263420 (+) 1083 WP_283266949.1 hypothetical protein -
  QLH32_RS15540 (QLH32_15540) - 3263420..3263743 (+) 324 WP_283266950.1 RnfH family protein -
  QLH32_RS15545 (QLH32_15545) bamE 3263797..3264195 (-) 399 WP_004676608.1 outer membrane protein assembly factor BamE -
  QLH32_RS15550 (QLH32_15550) fur 3264307..3264744 (+) 438 WP_004682786.1 ferric iron uptake transcriptional regulator -
  QLH32_RS15555 (QLH32_15555) pilU 3264846..3265964 (-) 1119 WP_279959583.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QLH32_RS15560 (QLH32_15560) pilT 3265990..3267027 (-) 1038 WP_267980244.1 type IV pilus twitching motility protein PilT Machinery gene
  QLH32_RS15565 (QLH32_15565) - 3267154..3267840 (+) 687 WP_283266951.1 YggS family pyridoxal phosphate-dependent enzyme -
  QLH32_RS15570 (QLH32_15570) - 3267837..3268628 (-) 792 WP_283266952.1 transglutaminase family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41782.94 Da        Isoelectric Point: 6.2891

>NTDB_id=832363 QLH32_RS15555 WP_279959583.1 3264846..3265964(-) (pilU) [Acinetobacter sp. KCTC 92772]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVAKTKLSGEIVGQLINSVMTDKQRKEFADTRECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPSMEDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFVHQHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNMRAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDVMKRSRELGMQTFDQ
ALFDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGADQLLSASKNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=832363 QLH32_RS15555 WP_279959583.1 3264846..3265964(-) (pilU) [Acinetobacter sp. KCTC 92772]
ATGGATTTTAATGATTTACTTAATCTGATGATTCAACAAAATGCATCGGACTTGTTTGTCACCGCAGATGTTGAACCGTC
GATGAAAATTAACGGGCAAATTGTGCCTGTTGCCAAAACGAAGTTATCGGGTGAAATTGTCGGTCAACTGATCAACTCAG
TGATGACTGACAAGCAACGTAAAGAATTTGCCGATACACGTGAATGTAACTTTGCCATCACCAACCGTGAAAAAACAGCA
CGCTTCCGTGTCAGCGCATTTCAACAACGTGATATGCCTGGGATGATCCTGCGTAAAATTGAAACGCATATTCCAAGCAT
GGAAGACCTGAAACTACCAGAAGTGCTCAAAGAACTTGCCATGACCAAGCGTGGCATCATTATCTTTGTGGGTGCAACAG
GTACAGGTAAATCCACCTCTTTGGCCTCCATGATTAGCTATCGCAACCACAATTCAAAAGGTCACATCATTACCATCGAG
GACCCAATTGAATTTGTACACCAACATGCGGGCTGTATCATCACCCAGCGCGAAGTGGGTATTGATACTGACTCATTTGA
AATCGCATTGAAGAATACCTTACGTCAGGCACCTGATGTCATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGACT
ATGCCATTGCCTTTGCGGAAACAGGTCACTTGGTATTAGCCACCCTACATGCCAACAACGCCAACCAGGCACTGGACCGT
ATCATTCACTTCTTTGAGGCAGATCGCCATAGCCAGCTTTATATGGACTTGTCGCTGAATATGAGAGCAATGGTCGCACA
ACAACTTATTCCAACCCCTGACGGTAATTCACGCCGTGCTGCCATTGAAATTTTGATCAATACACCATTATTGGCAGATT
ATATTCGTAAGGGCGAAATTCATGAGATTAAAGATGTCATGAAGCGTTCACGCGAACTCGGTATGCAGACCTTTGACCAA
GCCTTATTTGACCTTTATAAGGCAGGTGAAATCACCTACAAAGATGCACTTAAACATGCAGACTCGCCAAACGATTTACG
TCTCACCATCAAACTTTCAGAAGAAGGTGCAGACCAATTACTCAGTGCCAGCAAAAATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

74.868

100

0.761
  pilU Pseudomonas stutzeri DSM 10701

69.272

99.731

0.691
  pilU Vibrio cholerae strain A1552

52.703

99.462

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.329

93.011

0.384
  pilT Neisseria gonorrhoeae MS11

40.588

91.398

0.371
  pilT Neisseria meningitidis 8013

40.588

91.398

0.371
  pilT Legionella pneumophila strain Lp02

41.59

87.903

0.366
  pilT Legionella pneumophila strain ERS1305867

41.59

87.903

0.366
  pilT Pseudomonas aeruginosa PAK

38.728

93.011

0.36