Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L6418_RS12420 Genome accession   NZ_AP023423
Coordinates   2553866..2554909 (+) Length   347 a.a.
NCBI ID   WP_237247237.1    Uniprot ID   A0AAN1XCF2
Organism   Sideroxyarcus emersonii strain MIZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2548866..2559909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6418_RS12385 (MIZ01_2528) - 2548885..2549934 (-) 1050 WP_237247230.1 permease -
  L6418_RS12390 (MIZ01_2529) - 2550091..2550900 (-) 810 WP_237247231.1 inositol monophosphatase family protein -
  L6418_RS12395 (MIZ01_2530) - 2551023..2551307 (-) 285 WP_237247232.1 HU family DNA-binding protein -
  L6418_RS12400 (MIZ01_2531) - 2551461..2551745 (-) 285 WP_237247233.1 DUF167 domain-containing protein -
  L6418_RS12405 (MIZ01_2532) - 2551742..2552308 (-) 567 WP_237247234.1 YggT family protein -
  L6418_RS12410 (MIZ01_2533) proC 2552308..2553120 (-) 813 WP_237247235.1 pyrroline-5-carboxylate reductase -
  L6418_RS12415 (MIZ01_2534) - 2553130..2553813 (-) 684 WP_237247236.1 YggS family pyridoxal phosphate-dependent enzyme -
  L6418_RS12420 (MIZ01_2535) pilT 2553866..2554909 (+) 1044 WP_237247237.1 type IV pilus twitching motility protein PilT Machinery gene
  L6418_RS12425 (MIZ01_2536) pilU 2554943..2556079 (+) 1137 WP_237247238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L6418_RS12430 (MIZ01_2537) msrA 2556125..2556751 (-) 627 WP_269807815.1 peptide-methionine (S)-S-oxide reductase MsrA -
  L6418_RS12435 (MIZ01_2538) - 2556769..2558472 (-) 1704 WP_237247239.1 cytochrome c biogenesis protein DipZ -
  L6418_RS12440 (MIZ01_2539) msrB 2558469..2559008 (-) 540 WP_237247240.1 peptide-methionine (R)-S-oxide reductase MsrB -
  L6418_RS12445 (MIZ01_2540) - 2559136..2559843 (-) 708 WP_237247241.1 FecR family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38271.13 Da        Isoelectric Point: 6.9359

>NTDB_id=83175 L6418_RS12420 WP_237247237.1 2553866..2554909(+) (pilT) [Sideroxyarcus emersonii strain MIZ01]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDMRRINLPPMDHKEVHAMVYDIMNDGQRKHYEENKEVDFSFEVPNLAR
FRVNAFIQNRGAGAVMRTIPSKILTLEDLKAPAIFKDISEFPRGMVLVTGPTGSGKSTTLAAMVNHINENEMGHILTVED
PIEFVHESKKSLVNQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGSGRAAAHEIMICTPAIRNLIREAKVPQMYSAIQTGGNIGMQTLDQC
LSDLVKRNIVSSAEARGKAVNKDLFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=83175 L6418_RS12420 WP_237247237.1 2553866..2554909(+) (pilT) [Sideroxyarcus emersonii strain MIZ01]
ATGGATATCACCGAACTGCTCGCCTTCGGCGTGAAGAACAAAGCTTCCGACTTGCACCTCTCCGCCGGCCTGCCACCGAT
GATCCGCGTACACGGCGACATGCGCCGCATCAACCTGCCCCCGATGGATCACAAGGAAGTCCACGCCATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGCATTACGAAGAGAATAAGGAAGTCGACTTCTCGTTCGAAGTACCTAACCTGGCGCGC
TTCCGCGTCAATGCCTTCATCCAGAACCGCGGCGCCGGCGCCGTCATGCGGACCATTCCTTCCAAGATCCTGACGCTGGA
AGACCTGAAGGCGCCGGCCATCTTCAAGGACATCTCGGAGTTCCCGCGCGGCATGGTGCTGGTGACCGGCCCGACCGGCT
CAGGCAAATCCACCACGCTGGCGGCGATGGTGAACCACATCAACGAGAACGAGATGGGCCACATCCTGACCGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGAGCCTGGTCAACCAGCGTGAGGTCGGCCCGCATACGCTGTCGTTCAACAA
CGCGCTGCGTTCGGCACTGCGCGAAGACCCGGACGTGATCCTCGTCGGCGAGATGCGCGACCTCGAGACCATCCGCCTGG
CCATGACGGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCAGTTCTGCAGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGACGAGAAGGAGATGGTGCGCGCAATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
GCTGCTCAAGACCAAGGACGGTTCCGGCCGCGCTGCGGCGCACGAGATCATGATCTGCACCCCGGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGCCGCAGATGTATTCCGCCATCCAGACCGGCGGCAACATCGGCATGCAGACGCTGGACCAATGC
CTGTCCGACCTGGTCAAGCGCAACATCGTGTCTTCCGCCGAAGCCCGCGGCAAGGCGGTGAACAAGGATCTTTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Pseudomonas aeruginosa PAK

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Neisseria meningitidis 8013

71.72

98.847

0.709
  pilT Neisseria gonorrhoeae MS11

71.429

98.847

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.16

97.406

0.654
  pilT Vibrio cholerae strain A1552

67.16

97.406

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Pseudomonas stutzeri DSM 10701

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.667

96.83

0.403
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401

Multiple sequence alignment