Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L6418_RS12425 Genome accession   NZ_AP023423
Coordinates   2554943..2556079 (+) Length   378 a.a.
NCBI ID   WP_237247238.1    Uniprot ID   A0AAN1XC10
Organism   Sideroxyarcus emersonii strain MIZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549943..2561079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6418_RS12390 (MIZ01_2529) - 2550091..2550900 (-) 810 WP_237247231.1 inositol monophosphatase family protein -
  L6418_RS12395 (MIZ01_2530) - 2551023..2551307 (-) 285 WP_237247232.1 HU family DNA-binding protein -
  L6418_RS12400 (MIZ01_2531) - 2551461..2551745 (-) 285 WP_237247233.1 DUF167 domain-containing protein -
  L6418_RS12405 (MIZ01_2532) - 2551742..2552308 (-) 567 WP_237247234.1 YggT family protein -
  L6418_RS12410 (MIZ01_2533) proC 2552308..2553120 (-) 813 WP_237247235.1 pyrroline-5-carboxylate reductase -
  L6418_RS12415 (MIZ01_2534) - 2553130..2553813 (-) 684 WP_237247236.1 YggS family pyridoxal phosphate-dependent enzyme -
  L6418_RS12420 (MIZ01_2535) pilT 2553866..2554909 (+) 1044 WP_237247237.1 type IV pilus twitching motility protein PilT Machinery gene
  L6418_RS12425 (MIZ01_2536) pilU 2554943..2556079 (+) 1137 WP_237247238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L6418_RS12430 (MIZ01_2537) msrA 2556125..2556751 (-) 627 WP_269807815.1 peptide-methionine (S)-S-oxide reductase MsrA -
  L6418_RS12435 (MIZ01_2538) - 2556769..2558472 (-) 1704 WP_237247239.1 cytochrome c biogenesis protein DipZ -
  L6418_RS12440 (MIZ01_2539) msrB 2558469..2559008 (-) 540 WP_237247240.1 peptide-methionine (R)-S-oxide reductase MsrB -
  L6418_RS12445 (MIZ01_2540) - 2559136..2559843 (-) 708 WP_237247241.1 FecR family protein -
  L6418_RS12450 (MIZ01_2541) - 2560019..2560564 (+) 546 WP_237247242.1 bacteriohemerythrin -
  L6418_RS12455 (MIZ01_2542) - 2560702..2560977 (+) 276 WP_237247243.1 DUF2282 domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42291.35 Da        Isoelectric Point: 6.7403

>NTDB_id=83176 L6418_RS12425 WP_237247238.1 2554943..2556079(+) (pilU) [Sideroxyarcus emersonii strain MIZ01]
MERDQATELVHNLLRGMISQKASDLFITSGFPPAFKIDGRMTPVSSQALTSQHTQELARSIMNDRQAAEFESTHECNFAI
SPPGIGRFRVNVFMQQQRVGMVLRTITTKIPTFDELGLPNVLKDVVMTKRGLVILVGGTGSGKSTSLAAMLGHRNHNSYG
HIITIEDPVEFVHDHDKCIITHREVGVDTDNWQIALKNTLRQAPDVILIGEIRDRETMEYAVAFSETGHLCLATLHANSA
NQALDRIINFFPEDRREQLLMDLSLNIKALISQRLIPRRDGKGRAAAFEILLNSPLISDLIFKGHVHEIKEVMSRSRELG
MQTFDQALFDLHESGAISYEEALKNADSVNDLRLKIKLEGQASKDRNVLSGTENLRMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=83176 L6418_RS12425 WP_237247238.1 2554943..2556079(+) (pilU) [Sideroxyarcus emersonii strain MIZ01]
ATGGAAAGAGACCAGGCCACCGAACTGGTGCACAACCTGCTGCGCGGGATGATCTCGCAGAAGGCATCCGACCTGTTCAT
CACCTCTGGCTTCCCGCCCGCATTCAAGATTGACGGCAGGATGACGCCGGTGTCCAGCCAGGCGCTGACATCTCAGCACA
CGCAGGAACTGGCGCGCAGCATCATGAACGACCGCCAGGCGGCGGAGTTCGAATCCACGCACGAATGCAACTTTGCCATC
AGCCCGCCCGGAATCGGGCGCTTCCGCGTCAACGTGTTCATGCAGCAGCAACGCGTCGGCATGGTGCTGCGCACCATCAC
CACCAAGATCCCCACCTTCGACGAACTCGGCTTGCCCAACGTGCTGAAGGATGTCGTGATGACCAAGCGCGGCCTGGTGA
TCCTGGTCGGCGGCACCGGTTCGGGCAAGTCCACTTCGCTGGCCGCGATGCTGGGGCACCGCAACCACAACAGTTACGGC
CACATCATCACCATCGAGGACCCGGTGGAGTTCGTGCACGACCACGACAAGTGCATCATCACCCACCGCGAAGTGGGCGT
GGATACCGACAATTGGCAGATCGCACTGAAGAACACACTGCGGCAGGCGCCCGACGTCATCCTGATCGGCGAGATCCGCG
ACCGCGAGACGATGGAATATGCCGTCGCCTTCTCTGAAACCGGCCACCTGTGTCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGACCGGCGCGAGCAGTTGCTGATGGACCTGTCGCTCAACAT
CAAGGCGCTCATCTCGCAGCGCCTGATCCCCAGGCGCGACGGCAAGGGCCGCGCTGCCGCCTTCGAAATCCTGCTCAATT
CGCCGCTGATCTCCGACCTGATCTTCAAAGGCCACGTGCATGAGATCAAGGAAGTCATGTCCAGGTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGGCCTTGTTCGACCTGCACGAGAGCGGGGCGATCAGTTACGAGGAAGCGCTCAAGAACGCCGA
CTCCGTCAACGACCTGCGCCTGAAGATCAAGCTGGAAGGCCAGGCTTCCAAGGACCGGAACGTGCTCAGCGGCACGGAAA
ACCTGCGTATGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.584

91.534

0.582
  pilU Acinetobacter baylyi ADP1

60.845

93.915

0.571
  pilU Vibrio cholerae strain A1552

55.747

92.063

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Pseudomonas aeruginosa PAK

42.73

89.153

0.381
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Acinetobacter baylyi ADP1

41.593

89.683

0.373
  pilT Acinetobacter nosocomialis M2

41.742

88.095

0.368
  pilT Acinetobacter baumannii D1279779

41.742

88.095

0.368
  pilT Acinetobacter baumannii strain A118

41.742

88.095

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.265

87.831

0.362
  pilT Vibrio cholerae strain A1552

41.265

87.831

0.362

Multiple sequence alignment