Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QF117_RS07610 Genome accession   NZ_CP124614
Coordinates   332344..333381 (+) Length   345 a.a.
NCBI ID   WP_282388432.1    Uniprot ID   -
Organism   Vibrio sp. YMD68     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327344..338381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QF117_RS07580 (QF117_07580) hemW 327704..328876 (-) 1173 WP_282388427.1 radical SAM family heme chaperone HemW -
  QF117_RS07585 (QF117_07585) - 328882..329478 (-) 597 WP_282388428.1 XTP/dITP diphosphatase -
  QF117_RS07590 (QF117_07590) - 329508..329939 (-) 432 WP_282388429.1 DUF4426 domain-containing protein -
  QF117_RS07595 (QF117_07595) - 330017..330574 (-) 558 WP_017034529.1 YggT family protein -
  QF117_RS07600 (QF117_07600) proC 330642..331460 (-) 819 WP_282388430.1 pyrroline-5-carboxylate reductase -
  QF117_RS07605 (QF117_07605) - 331610..332320 (-) 711 WP_282388431.1 YggS family pyridoxal phosphate-dependent enzyme -
  QF117_RS07610 (QF117_07610) pilT 332344..333381 (+) 1038 WP_282388432.1 type IV pilus twitching motility protein PilT Machinery gene
  QF117_RS07615 (QF117_07615) pilU 333503..334609 (+) 1107 WP_282388433.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QF117_RS07620 (QF117_07620) ruvX 334782..335207 (-) 426 WP_282388434.1 Holliday junction resolvase RuvX -
  QF117_RS07625 (QF117_07625) - 335350..335913 (-) 564 WP_282388435.1 YqgE/AlgH family protein -
  QF117_RS07630 (QF117_07630) gshB 335974..336921 (-) 948 WP_282388436.1 glutathione synthase -
  QF117_RS07635 (QF117_07635) rsmE 336935..337666 (-) 732 WP_282388437.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QF117_RS07640 (QF117_07640) - 337849..338340 (-) 492 WP_282388438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38282.78 Da        Isoelectric Point: 6.3898

>NTDB_id=826766 QF117_RS07610 WP_282388432.1 332344..333381(+) (pilT) [Vibrio sp. YMD68]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHAEVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFNQTRGSSAVFRTIPVHIPTLEQIEAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHENNKCLVNQREVHKDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESMRAVIAQKLLKRVGGGRVACHEVMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAKGLVDPQEVAKKIEIESSSF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=826766 QF117_RS07610 WP_282388432.1 332344..333381(+) (pilT) [Vibrio sp. YMD68]
ATGGATATTGCAGAGTTATTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACACCTTTCTGCAGGGGTGCCTCCAAT
GGTACGGATAGATGGTGAAGTAAGAAAGCTTGGTGTTCCTGCTTTTAGCCATGCAGAGGTTCATCGACTTGTTTTTGAGA
TCATGAACGATGCTCAGCGCAGTGAATATGAAGAAAAGCTTGAAGTGGATTTTTCTTTTGAACTGCCGAATGTTGGCCGA
TTTCGTGTGAATGCGTTTAATCAGACGCGTGGTAGCTCTGCGGTATTTCGTACAATCCCAGTGCATATCCCTACCTTAGA
GCAAATTGAAGCTCCCGATATCTTTGAAAAAATAGCAAATTATGAAAAAGGCCTAGTGCTCGTCACAGGGCCTACAGGCT
CAGGTAAATCAACAACCTTAGCGGCGATGGTGGATTACATTAACCGTAATCATAATAAACACATTCTAACCATTGAAGAT
CCGATTGAATTTGTTCATGAGAACAACAAGTGCTTGGTGAATCAACGAGAAGTACACAAAGATACCCATAGTTTTAAGAA
TGCCCTCCGTTCGGCACTGCGTGAAGATCCAGACGTGATCTTAGTGGGTGAGCTTCGAGATCAAGAAACCATCAGTCTCG
CCTTAACAGCGGCTGAAACGGGGCATTTAGTTTTTGGTACGCTTCACACCAGCTCGGCGGCGAAAACTGTTGACCGGATT
ATCGATGTGTTTCCTGGTAGTGATAAAGACATGGTGCGTTCGATGCTTTCTGAATCAATGAGGGCGGTTATTGCGCAGAA
GCTATTGAAACGAGTCGGTGGGGGAAGGGTCGCCTGTCATGAGGTGATGCTTGCGACGCCAGCGATCCGAAACTTGATTC
GAGAAGACAAAGTGGCACAGATGTATTCGATTATTCAAACCGGTGCGGCCCATGGCATGCAAACCATGGAGCAAAATGCG
AAACAATTGATCGCAAAAGGTTTGGTTGATCCACAAGAAGTAGCGAAAAAAATCGAAATTGAATCATCGAGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

90.145

100

0.901
  pilT Vibrio cholerae strain A1552

90.145

100

0.901
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter nosocomialis M2

71.386

96.232

0.687
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.793

97.971

0.635
  pilT Neisseria gonorrhoeae MS11

63.112

100

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.568

100

0.499
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.768

95.072

0.397
  pilU Acinetobacter baylyi ADP1

39.375

92.754

0.365