Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QF117_RS07615 Genome accession   NZ_CP124614
Coordinates   333503..334609 (+) Length   368 a.a.
NCBI ID   WP_282388433.1    Uniprot ID   -
Organism   Vibrio sp. YMD68     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328503..339609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QF117_RS07585 (QF117_07585) - 328882..329478 (-) 597 WP_282388428.1 XTP/dITP diphosphatase -
  QF117_RS07590 (QF117_07590) - 329508..329939 (-) 432 WP_282388429.1 DUF4426 domain-containing protein -
  QF117_RS07595 (QF117_07595) - 330017..330574 (-) 558 WP_017034529.1 YggT family protein -
  QF117_RS07600 (QF117_07600) proC 330642..331460 (-) 819 WP_282388430.1 pyrroline-5-carboxylate reductase -
  QF117_RS07605 (QF117_07605) - 331610..332320 (-) 711 WP_282388431.1 YggS family pyridoxal phosphate-dependent enzyme -
  QF117_RS07610 (QF117_07610) pilT 332344..333381 (+) 1038 WP_282388432.1 type IV pilus twitching motility protein PilT Machinery gene
  QF117_RS07615 (QF117_07615) pilU 333503..334609 (+) 1107 WP_282388433.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QF117_RS07620 (QF117_07620) ruvX 334782..335207 (-) 426 WP_282388434.1 Holliday junction resolvase RuvX -
  QF117_RS07625 (QF117_07625) - 335350..335913 (-) 564 WP_282388435.1 YqgE/AlgH family protein -
  QF117_RS07630 (QF117_07630) gshB 335974..336921 (-) 948 WP_282388436.1 glutathione synthase -
  QF117_RS07635 (QF117_07635) rsmE 336935..337666 (-) 732 WP_282388437.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QF117_RS07640 (QF117_07640) - 337849..338340 (-) 492 WP_282388438.1 SprT family zinc-dependent metalloprotease -
  QF117_RS07645 (QF117_07645) - 338537..339334 (-) 798 WP_282388439.1 DUF2189 domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41233.17 Da        Isoelectric Point: 6.0641

>NTDB_id=826767 QF117_RS07615 WP_282388433.1 333503..334609(+) (pilU) [Vibrio sp. YMD68]
MELNQCLQGMITQKASDVYITVGAPILFRVDGELRAEGAKLGEAEVEQLLGAMMDQERASEYKQTREANFAIVRDIGRFR
VSAFYQRELPGAVIRRIETQIPTFEQLKLPDVLQNLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNANRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEVLLNSPRVSDLIRRGDLHELKTTMAKSKEIGMQTFDQALY
QLVVDGKISEQDALHSADSANDLRLMLKTQRGESFDTGTLQNVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=826767 QF117_RS07615 WP_282388433.1 333503..334609(+) (pilU) [Vibrio sp. YMD68]
ATGGAACTGAATCAGTGTTTGCAAGGCATGATTACGCAGAAAGCTTCGGATGTTTATATTACCGTTGGTGCGCCAATCCT
GTTTCGTGTTGACGGCGAGCTTAGAGCGGAAGGTGCCAAGTTAGGGGAAGCCGAGGTTGAGCAATTGCTGGGCGCAATGA
TGGATCAAGAGCGCGCTTCAGAATACAAGCAAACCCGAGAAGCGAACTTCGCAATTGTGCGAGATATCGGGCGATTTAGA
GTCAGTGCGTTTTATCAGCGAGAGTTGCCGGGCGCCGTAATTCGTCGTATTGAAACGCAAATTCCAACCTTTGAGCAGTT
GAAACTGCCCGATGTATTGCAAAACCTTTCAATCGCAAAGCGAGGTTTAGTTTTGGTGGTTGGAGCGACAGGCTCAGGGA
AGTCAACCACGATGGCCGCGATGACAGGGTACCGCAACGCGAATCGAACTGGGCATATTCTTACCGTTGAAGATCCTATC
GAGTTTGTCCATGAGCACCAACGCTGCATAGTAACCCAGCGTGAGGTCGGGCTTGACACTGAAAGCTATGAAGTTGCGCT
GAAAAACTCGCTGCGCCAAGCTCCTGACATGATTTTGATCGGTGAGATACGCAGTCGAGAAACCATGGAATACGCGATGA
CCTTTGCCGAAACGGGTCATTTATGCATGGCAACACTGCATGCTAATAATGCCAACCAAGCACTGGAACGTATTTTACAC
CTGGTCCCGAAAGAGCAAAAAGAGCAATTCTTGTTTGATCTTTCAATGAATCTAAAAGGTGTGATTGGTCAGCAGCTGAT
CCGTGACAAAAATGGTCAAGGCCGTCATGGTGTGTTTGAAGTGCTTTTGAATAGCCCAAGAGTATCGGATCTTATCCGTC
GTGGTGATCTTCATGAACTAAAAACAACGATGGCGAAGTCGAAAGAGATCGGAATGCAGACGTTTGATCAAGCTCTCTAC
CAGTTGGTGGTTGATGGAAAGATCAGCGAGCAGGATGCACTCCACAGCGCTGATTCTGCCAATGATCTGAGATTGATGTT
GAAAACTCAGCGTGGGGAAAGCTTCGATACTGGCACGTTGCAGAACGTTAAAATAGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.87

100

0.859
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.38

0.562
  pilU Acinetobacter baylyi ADP1

54.131

95.38

0.516
  pilT Acinetobacter baylyi ADP1

40.762

92.663

0.378
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Legionella pneumophila strain Lp02

40.708

92.12

0.375
  pilT Legionella pneumophila strain ERS1305867

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

40.413

92.12

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.975

88.043

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.761

0.367
  pilT Vibrio cholerae strain A1552

40.419

90.761

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361