Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K9V56_RS04775 Genome accession   NZ_CP124551
Coordinates   1091472..1092608 (+) Length   378 a.a.
NCBI ID   WP_102766116.1    Uniprot ID   -
Organism   Kinneretia aquatilis strain DH15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1086472..1097608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9V56_RS04755 (K9V56_004755) - 1087284..1088021 (-) 738 WP_223696759.1 c-type cytochrome -
  K9V56_RS04760 (K9V56_004760) - 1088096..1089517 (-) 1422 WP_223696758.1 PLP-dependent aminotransferase family protein -
  K9V56_RS04765 (K9V56_004765) - 1089514..1090257 (-) 744 WP_171827714.1 YggS family pyridoxal phosphate-dependent enzyme -
  K9V56_RS04770 (K9V56_004770) pilT 1090284..1091327 (+) 1044 WP_102766115.1 type IV pilus twitching motility protein PilT Machinery gene
  K9V56_RS04775 (K9V56_004775) pilU 1091472..1092608 (+) 1137 WP_102766116.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K9V56_RS04780 (K9V56_004780) - 1092658..1093599 (+) 942 WP_171827713.1 NAD(P)-dependent oxidoreductase -
  K9V56_RS04785 (K9V56_004785) - 1093653..1094297 (-) 645 WP_223696757.1 BON domain-containing protein -
  K9V56_RS04790 (K9V56_004790) - 1094378..1094950 (-) 573 WP_102766119.1 SIS domain-containing protein -
  K9V56_RS04795 (K9V56_004795) - 1095060..1095503 (-) 444 WP_102766577.1 YraN family protein -
  K9V56_RS04800 (K9V56_004800) rsmI 1095496..1096416 (+) 921 WP_171827711.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  K9V56_RS04805 (K9V56_004805) - 1096470..1096829 (-) 360 WP_171827710.1 hypothetical protein -
  K9V56_RS04810 (K9V56_004810) - 1097068..1097538 (-) 471 WP_171827709.1 DUF4188 domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42156.34 Da        Isoelectric Point: 6.7025

>NTDB_id=826428 K9V56_RS04775 WP_102766116.1 1091472..1092608(+) (pilU) [Kinneretia aquatilis strain DH15]
MERDQASKFINDLLRLMISRKGSDLFLTAEFPPAIKVDGKVTKVSPQPLTGQHTLQLARSIMNDKQAAEFERNKECNFAI
SPQGIGRFRVNAFLQQGSVGLVLRTIPADLPTIASLDLPPVLREVVQFKRGLVIVVGATGSGKSTSLAAMIDERNQTTFG
HIITIEDPIEFVHPHKNCIVTQREVGIDTDSWESALKNSLRQAPDVILMGELRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRSQLLMDLSLNLRALVSQRLLPRREGKGRVAAVEILLNTPLVSDLIFKGEVGEIKEIMKRSREQG
MQTFDQALFDLYESGKVTYEDALRNADSVNDLRLQIKLNSERARSGDLSTGTEHFTIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=826428 K9V56_RS04775 WP_102766116.1 1091472..1092608(+) (pilU) [Kinneretia aquatilis strain DH15]
ATGGAACGCGATCAGGCCTCGAAATTCATCAACGATCTGCTGCGCCTGATGATCTCGCGCAAGGGCTCGGATCTGTTCCT
CACGGCCGAGTTTCCGCCCGCCATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCCCTGACCGGCCAGCACA
CGCTGCAGCTGGCGCGTTCCATCATGAATGACAAGCAGGCGGCCGAGTTCGAGCGCAACAAGGAATGCAATTTCGCCATC
TCGCCGCAAGGCATTGGTCGCTTCCGCGTCAATGCCTTTTTGCAGCAGGGCTCGGTGGGCCTGGTGCTGCGGACGATTCC
GGCCGATCTGCCGACCATTGCCTCGCTGGACCTGCCGCCGGTGCTGCGCGAAGTGGTGCAGTTCAAGCGCGGCCTGGTGA
TCGTGGTGGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCCGCCATGATCGATGAGCGCAACCAGACCACCTTCGGC
CACATCATCACCATCGAAGACCCGATCGAGTTCGTGCATCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTTGGCAT
CGACACCGACAGCTGGGAGTCGGCCCTGAAGAACTCCCTGCGCCAGGCGCCCGATGTGATCCTGATGGGCGAGTTGCGCG
ACCGCGAGACCATGGAGCATGCCGTGGCCTTCGCCGAAACCGGTCACCTTTGCATGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGGCGCAGCCAACTGCTGATGGACCTGTCGCTCAATCT
GCGTGCCCTGGTCTCGCAGCGCCTGTTGCCGCGCCGCGAGGGCAAGGGCCGGGTGGCAGCGGTCGAGATCTTGCTCAACA
CACCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGAGATCATGAAGCGCTCGCGCGAGCAGGGC
ATGCAGACCTTTGACCAGGCCTTGTTCGATCTCTACGAGAGCGGCAAGGTCACCTACGAAGACGCGTTGCGCAATGCCGA
TTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCGAGCGCGCGCGTTCCGGCGATCTGAGCACCGGCACCGAGC
ACTTCACCATCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.736

96.296

0.614
  pilU Acinetobacter baylyi ADP1

58.939

94.709

0.558
  pilU Vibrio cholerae strain A1552

53.719

96.032

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.071

89.153

0.429
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.976

87.831

0.386
  pilT Vibrio cholerae strain A1552

43.976

87.831

0.386
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

42.342

88.095

0.373
  pilT Acinetobacter baumannii D1279779

42.342

88.095

0.373
  pilT Acinetobacter baumannii strain A118

42.342

88.095

0.373
  pilT Neisseria gonorrhoeae MS11

40.413

89.683

0.362
  pilT Neisseria meningitidis 8013

40.413

89.683

0.362