Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QJS82_RS10615 Genome accession   NZ_CP124526
Coordinates   2496387..2497442 (+) Length   351 a.a.
NCBI ID   WP_149490560.1    Uniprot ID   -
Organism   Psychrobacter sp. WB2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491387..2502442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJS82_RS10600 (QJS82_10600) - 2492971..2493672 (+) 702 WP_200662116.1 IS1 family transposase -
  QJS82_RS10605 (QJS82_10605) - 2493878..2495155 (+) 1278 WP_281490449.1 hemolysin family protein -
  QJS82_RS10610 (QJS82_10610) - 2495246..2496025 (-) 780 WP_281490450.1 YggS family pyridoxal phosphate-dependent enzyme -
  QJS82_RS10615 (QJS82_10615) pilT 2496387..2497442 (+) 1056 WP_149490560.1 type IV pilus twitching motility protein PilT Machinery gene
  QJS82_RS10620 (QJS82_10620) pilU 2497617..2498738 (+) 1122 WP_201619098.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QJS82_RS10625 (QJS82_10625) fur 2498866..2499300 (-) 435 WP_201562358.1 ferric iron uptake transcriptional regulator -
  QJS82_RS10630 (QJS82_10630) bamE 2499625..2500044 (+) 420 WP_183619781.1 outer membrane protein assembly factor BamE -
  QJS82_RS10635 (QJS82_10635) - 2500095..2500511 (-) 417 WP_281490451.1 RnfH family protein -
  QJS82_RS10640 (QJS82_10640) - 2500511..2501653 (-) 1143 WP_281490452.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39194.05 Da        Isoelectric Point: 6.6713

>NTDB_id=826220 QJS82_RS10615 WP_149490560.1 2496387..2497442(+) (pilT) [Psychrobacter sp. WB2]
MAEKNNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPEMSHQVVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMI
TLDQTLKNLVSKGTISKDVARPYAKQPEAFI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=826220 QJS82_RS10615 WP_149490560.1 2496387..2497442(+) (pilT) [Psychrobacter sp. WB2]
ATGGCTGAAAAGAATAATTTAAGCATCGAAGACTTGCTGCGCTTTACGGTCAAGCATAAAGCCTCAGATTTACATATTTC
AGCCGGTATGCCAGCGATGATTCGTGTCGATGGTGAGATGACCCGTATCAATATGCCAGAGATGAGCCATCAGGTTGTGC
ATCAGCTTATTTATGACATCATGAATGATAAGCAACGTGCTGACTTTGAAGAGTTTTTTGAGACCGACTTCTCTTTTGAG
ATTCCAAATTTGGCGCGCTTTCGTGTTAACGCCTTTAACCAAAATCGCGGCGCAGGCGCAGTCTTTCGTACCATTCCCTC
TAAAGTCCTTACCATGGAAGACTTGGGTATGGGCGAGGTTTTCAAACGTGTGTCTGACTTTAAACGCGGCGTAGTATTGG
TTACAGGTCCGACGGGTTCAGGGAAGTCTACCACGCTGGCGGCCATGATTGATTATATCAATGAAACTCGTAAAGAGCAT
ATTTTAACCATTGAAGACCCGATTGAATTTGTCCACGAGTCTAAAAAGAGCTTGATTAACCAACGTGAAGTCCACCGCGA
TACCTTAGGTTTCGAGCCTGCGTTGCGCTCTGCGTTACGTGAAGATCCAGACGTTATCCTTGTTGGTGAGCTTCGTGACC
TTGAAACTATTCGCTTAGCTTTGACCGCCGCAGAAACAGGGCACTTGGTCTTTGGTACTTTGCACACCAGCTCAGCGGCA
AAAACCATTGACCGTGTGATTGACGTCTTCCCTGCTGCTGAAAAAGACATGATTCGAGCGATGCTTTCGGAGTCATTACA
AGCGGTCATCTCACAGACTCTTTTGCGTCGTCAAACCGGCGGACGAATTGCCGCGCATGAGATTATGCTAGGCACGCCTG
CTATTCGTAACTTGATACGTGAAAACAAAATCGCCCAGATGTACTCCTCTATTCAGACGGGTGCTGGTGATGGCATGATT
ACCCTTGACCAAACGCTTAAAAATCTTGTCTCAAAAGGTACTATTAGCAAAGATGTTGCACGTCCTTATGCCAAGCAGCC
TGAGGCGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

98.291

0.775
  pilT Acinetobacter baumannii D1279779

78.551

98.291

0.772
  pilT Acinetobacter baumannii strain A118

78.551

98.291

0.772
  pilT Pseudomonas stutzeri DSM 10701

77.907

98.006

0.764
  pilT Pseudomonas aeruginosa PAK

77.907

98.006

0.764
  pilT Acinetobacter baylyi ADP1

76.453

98.006

0.749
  pilT Legionella pneumophila strain Lp02

71.802

98.006

0.704
  pilT Legionella pneumophila strain ERS1305867

71.802

98.006

0.704
  pilT Neisseria meningitidis 8013

64.928

98.291

0.638
  pilT Neisseria gonorrhoeae MS11

64.348

98.291

0.632
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.173

93.732

0.63
  pilT Vibrio cholerae strain A1552

67.173

93.732

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.72

98.86

0.501
  pilU Acinetobacter baylyi ADP1

41.691

97.721

0.407
  pilU Vibrio cholerae strain A1552

43.344

92.023

0.399
  pilU Pseudomonas stutzeri DSM 10701

41.003

96.581

0.396