Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QJS82_RS10620 Genome accession   NZ_CP124526
Coordinates   2497617..2498738 (+) Length   373 a.a.
NCBI ID   WP_201619098.1    Uniprot ID   -
Organism   Psychrobacter sp. WB2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2492617..2503738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJS82_RS10600 (QJS82_10600) - 2492971..2493672 (+) 702 WP_200662116.1 IS1 family transposase -
  QJS82_RS10605 (QJS82_10605) - 2493878..2495155 (+) 1278 WP_281490449.1 hemolysin family protein -
  QJS82_RS10610 (QJS82_10610) - 2495246..2496025 (-) 780 WP_281490450.1 YggS family pyridoxal phosphate-dependent enzyme -
  QJS82_RS10615 (QJS82_10615) pilT 2496387..2497442 (+) 1056 WP_149490560.1 type IV pilus twitching motility protein PilT Machinery gene
  QJS82_RS10620 (QJS82_10620) pilU 2497617..2498738 (+) 1122 WP_201619098.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QJS82_RS10625 (QJS82_10625) fur 2498866..2499300 (-) 435 WP_201562358.1 ferric iron uptake transcriptional regulator -
  QJS82_RS10630 (QJS82_10630) bamE 2499625..2500044 (+) 420 WP_183619781.1 outer membrane protein assembly factor BamE -
  QJS82_RS10635 (QJS82_10635) - 2500095..2500511 (-) 417 WP_281490451.1 RnfH family protein -
  QJS82_RS10640 (QJS82_10640) - 2500511..2501653 (-) 1143 WP_281490452.1 hypothetical protein -
  QJS82_RS10645 (QJS82_10645) - 2501909..2502577 (-) 669 WP_281490453.1 c-type cytochrome -
  QJS82_RS10650 (QJS82_10650) - 2502934..2503272 (-) 339 WP_281490454.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41714.97 Da        Isoelectric Point: 6.9359

>NTDB_id=826221 QJS82_RS10620 WP_201619098.1 2497617..2498738(+) (pilU) [Psychrobacter sp. WB2]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTPSQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRESGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSNKNGTANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=826221 QJS82_RS10620 WP_201619098.1 2497617..2498738(+) (pilU) [Psychrobacter sp. WB2]
ATGGACATTGATAAACTTTTACAGTTAATGATCAATAAAAACGGCTCTGACCTTTTTATTACCGCTGACGTTGCGCCATC
CATGAAAATTAATGGCAAAATTCTCCCCGTGGGTAAAACGCCATTAACGGCTGAGCAAACCATGAGATTGGTTAAAGGGG
TGATGACGCCAAGCCAACAAAAAGAGTTCGATGAAACCAATGAGTGTCAGTTTGCGATTACAGACCAAGCTCAGCAAGCG
CGTTTTCGGGTTAGTGCTTTTATACAGCGCGACATGGCGGGGATGATTTTACGGAAAATTGAGAATAAAATTCCGACAGT
CGAAGAGCTGCGCCTGCCACCAGCATTAAAAGAGCTGGCCATGAAAAAGCGCGGTATTATCTTGTTGGTTGGTGCAACAG
GTACCGGTAAATCGACCACCCTTGCGGCAATGATTGGGCACCGTAACCAGAACTCACATGGTCATATTATTACCATCGAA
GATCCTATCGAGTTTGTCCATAAGCATCGCGGTTGTATCATTACTCAGCGTGAAGTCGGTATCGACACCCACTCTTTTGA
AGCGGGGCTGAAAAACACCTTACGCCAAGCGCCTGATGTTATTTTGATTGGCGAGATTCGTAACCGCGAGGTTATGAGCT
ACGCGATTCAATATGCGGAGACCGGACATTTGGTCTTTGCTACCTTACACGCCAATAACGCCAACCAAGCGATTGACCGT
ATTATTCACTTCTTTGAAACCAGTCGCCATAATCAGTTGTTTATGGATTTATCGCTGAATTTACAAGCTATTATCGCTCA
GCAACTGATTCCAACGCCTGATGGCAAAGGACGCCGCGCTGCTATTGAGATTTTATTAAACTCACAGCTAATTAGTGACT
ATATTCGTAAAGGCGAGGTTCATGAAATCAAAGATGTGATGACGCGCTCACGCGAGAGCGGTATGCAGACCTTTGACCAA
GCGCTGTTTGATTTGTATGAACAGGGCGAAATCACCTATAAAGAAGCCATTCTGCACGCCGACTCGCCTAATGATTTGCG
CTTAAAAATCAAATTGAGCAATAAAAATGGCACCGCCAATTTAGATGAAGGCGCTGATAGTTTATCGCTAGATATTACTT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.684

100

0.657
  pilU Acinetobacter baylyi ADP1

67.324

95.174

0.641
  pilU Vibrio cholerae strain A1552

52.446

98.66

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.803

98.123

0.41
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

89.812

0.37
  pilT Vibrio cholerae strain A1552

41.194

89.812

0.37
  pilT Acinetobacter nosocomialis M2

39.942

91.957

0.367
  pilT Acinetobacter baumannii D1279779

39.942

91.957

0.367
  pilT Acinetobacter baumannii strain A118

39.942

91.957

0.367
  pilT Legionella pneumophila strain Lp02

40.774

90.08

0.367
  pilT Legionella pneumophila strain ERS1305867

40.774

90.08

0.367
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362