Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QAO71_RS00810 Genome accession   NZ_CP123445
Coordinates   175059..176093 (+) Length   344 a.a.
NCBI ID   WP_036991401.1    Uniprot ID   A0A4U0YKI8
Organism   Halopseudomonas sp. SMJS2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 170059..181093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO71_RS00780 (QAO71_00780) - 170616..171065 (-) 450 WP_074779176.1 DUF4426 domain-containing protein -
  QAO71_RS00785 (QAO71_00785) metW 171078..171668 (-) 591 WP_036992029.1 methionine biosynthesis protein MetW -
  QAO71_RS00790 (QAO71_00790) - 171671..172813 (-) 1143 WP_036991407.1 homoserine O-acetyltransferase -
  QAO71_RS00795 (QAO71_00795) - 172859..173449 (-) 591 WP_036991406.1 YggT family protein -
  QAO71_RS00800 (QAO71_00800) proC 173459..174277 (-) 819 WP_036991405.1 pyrroline-5-carboxylate reductase -
  QAO71_RS00805 (QAO71_00805) - 174274..174980 (-) 707 Protein_160 YggS family pyridoxal phosphate-dependent enzyme -
  QAO71_RS00810 (QAO71_00810) pilT 175059..176093 (+) 1035 WP_036991401.1 type IV pilus twitching motility protein PilT Machinery gene
  QAO71_RS00815 (QAO71_00815) pilU 176139..177278 (+) 1140 WP_036991400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QAO71_RS00820 (QAO71_00820) - 177297..178568 (-) 1272 WP_280165037.1 dihydroorotase -
  QAO71_RS00825 (QAO71_00825) - 178571..179566 (-) 996 WP_280165038.1 aspartate carbamoyltransferase catalytic subunit -
  QAO71_RS00830 (QAO71_00830) pyrR 179574..180086 (-) 513 WP_036991395.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QAO71_RS00835 (QAO71_00835) ruvX 180083..180523 (-) 441 WP_136868477.1 Holliday junction resolvase RuvX -
  QAO71_RS00840 (QAO71_00840) - 180523..181092 (-) 570 WP_036991392.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37883.65 Da        Isoelectric Point: 6.8853

>NTDB_id=821122 QAO71_RS00810 WP_036991401.1 175059..176093(+) (pilT) [Halopseudomonas sp. SMJS2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINVPALEHKQVHALIYDIMNDRQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGDVFKKISDVPRGLVLVTGPTGSGKSTTLAAMIDHLNCNKYQHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGNIGMQTLDMCL
KNLVSRGLVTREAAREKAKAGESL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=821122 QAO71_RS00810 WP_036991401.1 175059..176093(+) (pilT) [Halopseudomonas sp. SMJS2]
ATGGATATAACCGAGCTGTTAGCCTTCAGCGCCAAACAGGGCGCGTCGGACTTACACCTGTCGGCCGGATTGCCGCCCAT
GATTCGAGTGGATGGCGATGTGCGTCGGATCAACGTACCGGCACTGGAACACAAGCAGGTTCATGCGCTGATCTACGACA
TCATGAATGATCGCCAGCGCAAGGATTTCGAGGAATTTCTCGAAACCGACTTCTCCTTCGAAGTGCCCGGCGTGGCCCGC
TTCCGGGTTAACGCCTTCAACCAGAATCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
AGAGCTGGGGATGGGCGACGTGTTCAAGAAGATCTCCGATGTGCCTCGCGGGCTGGTGCTGGTCACCGGACCGACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGATCATCTGAACTGCAACAAGTACCAGCACATTCTCACCATCGAAGAC
CCGATCGAATTCGTCCACGAATCAAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGATACCCACGGTTTCTCCGA
AGCCCTGCGCTCGGCCCTGCGCGAAGACCCCGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACTGCGGCAGAGACTGGTCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCGGCGAGGAAAAGGCTATGGTCCGCTCGATGCTCTCCGAATCATTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTGGCAGCCCACGAAATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGCAATATCGGCATGCAGACGCTGGATATGTGCCTG
AAGAACCTGGTATCCCGCGGTTTGGTTACCCGGGAAGCTGCGCGCGAGAAAGCCAAGGCGGGCGAAAGCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U0YKI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

92.151

100

0.922
  pilT Pseudomonas stutzeri DSM 10701

89.826

100

0.898
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.687

98.547

0.529
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384