Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QAO71_RS00815 Genome accession   NZ_CP123445
Coordinates   176139..177278 (+) Length   379 a.a.
NCBI ID   WP_036991400.1    Uniprot ID   A0A031MCY8
Organism   Halopseudomonas sp. SMJS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 171139..182278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO71_RS00790 (QAO71_00790) - 171671..172813 (-) 1143 WP_036991407.1 homoserine O-acetyltransferase -
  QAO71_RS00795 (QAO71_00795) - 172859..173449 (-) 591 WP_036991406.1 YggT family protein -
  QAO71_RS00800 (QAO71_00800) proC 173459..174277 (-) 819 WP_036991405.1 pyrroline-5-carboxylate reductase -
  QAO71_RS00805 (QAO71_00805) - 174274..174980 (-) 707 Protein_160 YggS family pyridoxal phosphate-dependent enzyme -
  QAO71_RS00810 (QAO71_00810) pilT 175059..176093 (+) 1035 WP_036991401.1 type IV pilus twitching motility protein PilT Machinery gene
  QAO71_RS00815 (QAO71_00815) pilU 176139..177278 (+) 1140 WP_036991400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QAO71_RS00820 (QAO71_00820) - 177297..178568 (-) 1272 WP_280165037.1 dihydroorotase -
  QAO71_RS00825 (QAO71_00825) - 178571..179566 (-) 996 WP_280165038.1 aspartate carbamoyltransferase catalytic subunit -
  QAO71_RS00830 (QAO71_00830) pyrR 179574..180086 (-) 513 WP_036991395.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QAO71_RS00835 (QAO71_00835) ruvX 180083..180523 (-) 441 WP_136868477.1 Holliday junction resolvase RuvX -
  QAO71_RS00840 (QAO71_00840) - 180523..181092 (-) 570 WP_036991392.1 YqgE/AlgH family protein -
  QAO71_RS00845 (QAO71_00845) - 181175..182080 (-) 906 WP_036992026.1 energy transducer TonB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42216.61 Da        Isoelectric Point: 6.7792

>NTDB_id=821123 QAO71_RS00815 WP_036991400.1 176139..177278(+) (pilU) [Halopseudomonas sp. SMJS2]
MEFEKLLRLMVEKGASDLFITAGVAPSMKVHGKILPVTKTVLSPEQTRETVLGVMSEAQRREFAENKECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIEVNIPTMEELRLPEILKQLSMTKRGLVIFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
IHFFPTEMHQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAAIEVLLNTPLAADMIRKGEVHELKPLMARSNEVGMQTFDQA
LYKLYSQGEITYEDALAHADSANDLRLLIKLGSETDGKHLMGKAVDGLALEDSDDNRRY

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=821123 QAO71_RS00815 WP_036991400.1 176139..177278(+) (pilU) [Halopseudomonas sp. SMJS2]
ATGGAGTTTGAAAAACTGCTGCGACTGATGGTCGAGAAGGGCGCTTCCGACCTGTTCATCACCGCTGGTGTGGCGCCGAG
CATGAAAGTGCACGGCAAGATCCTGCCGGTGACCAAGACTGTATTGTCACCGGAACAGACCCGGGAAACCGTGCTGGGGG
TGATGTCCGAGGCTCAGCGCCGGGAATTCGCCGAGAACAAGGAATGCAACTTTGCGATCAGCGCCCGGGGTGTCGGTCGC
TTTCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGGATCGAGGTCAATATCCCAACCATGGA
AGAGCTGCGGCTGCCGGAGATCCTCAAGCAGCTGTCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGTAAATCCACTTCGCTGGCATCGATGATCGGCTATCGCAACAAGAACTCCAGCGGACACATCATCTCCATCGAAGAC
CCGATCGAATTCATTCACCAGCATCAGAATTGCATCGTCACCCAGCGGGAAGTGGGCATCGATACCGATTCCTTTGAGGT
GGCGCTGAAGAATACCCTGCGCCAGGCGCCGGATGTGATCCTGATCGGTGAGGTGCGGACCCGCGAAACCATGGACCATG
CGGTGGCCTTTGCCGAAACCGGCCACCTATGTCTGGCCACGCTGCATGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCCACTTCTTCCCCACCGAGATGCATCAGCAGGTGTGGATGGACCTGTCACTCAATCTCAAGGCCATCGTCGCCCAGCA
GCTGATCCCGACGCCGGATGGCAAGGGTCGCCGTGCCGCCATCGAGGTGTTGCTCAACACCCCATTGGCCGCCGACATGA
TTCGCAAGGGCGAAGTGCATGAGCTCAAGCCGCTGATGGCGCGTTCCAACGAAGTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAAACTGTACAGCCAAGGCGAGATCACCTACGAAGATGCACTGGCTCACGCCGATTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTGGGCTCGGAGACCGATGGCAAGCATCTGATGGGCAAGGCGGTAGACGGCCTGGCACTGGAAGACTCGG
ATGATAACCGGCGGTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A031MCY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

85.372

99.208

0.847
  pilU Acinetobacter baylyi ADP1

65.826

94.195

0.62
  pilU Vibrio cholerae strain A1552

59.143

92.348

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

89.446

0.388