Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R5028_RS20200 Genome accession   NZ_CP137486
Coordinates   4294480..4295514 (-) Length   344 a.a.
NCBI ID   WP_003084552.1    Uniprot ID   A0A0H2ZKV1
Organism   Pseudomonas aeruginosa strain HPA2660     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4289480..4300514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5028_RS20180 (R5028_20180) - 4290073..4291446 (-) 1374 WP_003100955.1 pyridoxal-phosphate dependent enzyme -
  R5028_RS20185 (R5028_20185) - 4291689..4292093 (-) 405 WP_003084560.1 NINE protein -
  R5028_RS20190 (R5028_20190) czcD 4292247..4293146 (+) 900 WP_003100957.1 CDF family zinc transporter CzcD -
  R5028_RS20195 (R5028_20195) pilU 4293154..4294302 (-) 1149 WP_003100959.1 type IV pilus ATPase PilU Machinery gene
  R5028_RS20200 (R5028_20200) pilT 4294480..4295514 (-) 1035 WP_003084552.1 type IV pilus twitching motility protein PilT Machinery gene
  R5028_RS20205 (R5028_20205) - 4295729..4296421 (+) 693 WP_003084549.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5028_RS20210 (R5028_20210) proC 4296433..4297254 (+) 822 WP_003084546.1 pyrroline-5-carboxylate reductase -
  R5028_RS20215 (R5028_20215) - 4297265..4297858 (+) 594 WP_003084544.1 YggT family protein -
  R5028_RS20220 (R5028_20220) - 4297855..4298151 (+) 297 WP_031628111.1 DUF167 domain-containing protein -
  R5028_RS20225 (R5028_20225) - 4298259..4300226 (+) 1968 WP_003084539.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38020.89 Da        Isoelectric Point: 6.8364

>NTDB_id=820578 R5028_RS20200 WP_003084552.1 4294480..4295514(-) (pilT) [Pseudomonas aeruginosa strain HPA2660]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGEVFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQSVISQTLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLISRENAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=820578 R5028_RS20200 WP_003084552.1 4294480..4295514(-) (pilT) [Pseudomonas aeruginosa strain HPA2660]
ATGGATATTACCGAGCTGCTCGCCTTCAGTGCCAAACAGGGCGCTTCGGACCTGCACCTCTCCGCCGGCCTGCCGCCCAT
GATCCGGGTGGATGGCGATGTACGCCGGATCAACCTGCCACCGCTGGAACACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
GGAGCTTGGCATGGGAGAAGTGTTCAAACGTGTTTCAGACGTCCCGCGCGGGCTGGTACTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTCGGCTTCAGCGA
AGCGCTGCGCTCGGCGCTGCGGGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCGGAGACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCCATGGTTCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GTGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGATCAGCCGCGAGAACGCCCGCGAGAAGGCGAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZKV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

100

100

1
  pilT Pseudomonas stutzeri DSM 10701

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.765

98.837

0.66
  pilT Vibrio cholerae strain A1552

66.765

98.837

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.298

98.547

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395