Detailed information NTG0820577

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5028_RS20195
• Genome accession: NZ_CP137486
• Coordinates: 4293154..4294302 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA2660
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4288154..4299302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5028_RS20175 (R5028_20175)	-	4288892..4290076 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
R5028_RS20180 (R5028_20180)	-	4290073..4291446 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
R5028_RS20185 (R5028_20185)	-	4291689..4292093 (-)	405	WP_003084560.1	NINE protein	-
R5028_RS20190 (R5028_20190)	czcD	4292247..4293146 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
R5028_RS20195 (R5028_20195)	pilU	4293154..4294302 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5028_RS20200 (R5028_20200)	pilT	4294480..4295514 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5028_RS20205 (R5028_20205)	-	4295729..4296421 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5028_RS20210 (R5028_20210)	proC	4296433..4297254 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5028_RS20215 (R5028_20215)	-	4297265..4297858 (+)	594	WP_003084544.1	YggT family protein	-
R5028_RS20220 (R5028_20220)	-	4297855..4298151 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=820577 R5028_RS20195 WP_003100959.1 4293154..4294302(-) (pilU) [Pseudomonas aeruginosa strain HPA2660]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=820577 R5028_RS20195 WP_003100959.1 4293154..4294302(-) (pilU) [Pseudomonas aeruginosa strain HPA2660]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382