Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HSX14_RS06180 Genome accession   NZ_AP023189
Coordinates   1326045..1326458 (-) Length   137 a.a.
NCBI ID   WP_173173299.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain TUM18999     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1321045..1331458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX14_RS06150 (TUM18999_12210) - 1321125..1321340 (+) 216 WP_173173309.1 hypothetical protein -
  HSX14_RS06155 (TUM18999_12220) yacG 1321344..1321544 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  HSX14_RS06160 (TUM18999_12230) coaE 1321541..1322152 (-) 612 WP_173173307.1 dephospho-CoA kinase -
  HSX14_RS06165 (TUM18999_12240) pilD 1322152..1323021 (-) 870 WP_173173305.1 prepilin peptidase Machinery gene
  HSX14_RS06170 (TUM18999_12250) pilC 1323022..1324242 (-) 1221 WP_173173303.1 type II secretion system F family protein Machinery gene
  HSX14_RS06175 (TUM18999_12260) pilB 1324246..1325949 (-) 1704 WP_173173301.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HSX14_RS06180 (TUM18999_12270) pilA 1326045..1326458 (-) 414 WP_173173299.1 pilin Machinery gene
  HSX14_RS06185 (TUM18999_12280) - 1326609..1328321 (+) 1713 WP_173173297.1 PglL family O-oligosaccharyltransferase -
  HSX14_RS06195 (TUM18999_12290) nadC 1328498..1329346 (-) 849 WP_173173295.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14258.23 Da        Isoelectric Point: 9.1461

>NTDB_id=81579 HSX14_RS06180 WP_173173299.1 1326045..1326458(-) (pilA) [Pseudomonas tohonis strain TUM18999]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIARAEANTGLQAIAPLKTAVEDQFSRGVGGASTTVANLGSGATASPL
GTITATFTSSGTGTLVFVFDRQASPRVKDSRITLNRASDGTWSCTSDVNSEYRPRGC

Nucleotide


Download         Length: 414 bp        

>NTDB_id=81579 HSX14_RS06180 WP_173173299.1 1326045..1326458(-) (pilA) [Pseudomonas tohonis strain TUM18999]
ATGAAAGCCCAAAAAGGTTTTACCCTGATTGAACTGATGATCGTCGTGGCGATCATCGGCATTCTGGCCGCTATCGCGAT
TCCGGCCTACCAGAACTACATTGCGCGCGCCGAAGCCAACACTGGCCTGCAGGCAATCGCCCCCCTGAAGACTGCTGTTG
AAGATCAGTTCTCCCGTGGAGTGGGTGGTGCCTCCACTACCGTTGCAAACCTTGGCAGCGGCGCCACCGCCAGCCCGCTG
GGTACAATCACTGCGACCTTCACCTCCAGCGGTACCGGCACGCTCGTGTTCGTTTTCGATCGACAAGCCAGCCCGCGCGT
AAAAGACTCGCGTATTACCCTGAATCGTGCCAGCGACGGTACTTGGTCCTGCACTTCTGACGTAAACTCGGAATACCGTC
CGCGCGGTTGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

50.676

100

0.547
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.521

100

0.474
  pilA Vibrio cholerae strain A1552

44.521

100

0.474
  pilA Vibrio cholerae C6706

44.521

100

0.474
  pilA Acinetobacter baumannii strain A118

45.714

100

0.467
  comP Acinetobacter baylyi ADP1

42.568

100

0.46
  pilA2 Legionella pneumophila str. Paris

42.282

100

0.46
  pilA2 Legionella pneumophila strain ERS1305867

40.94

100

0.445
  pilA Acinetobacter nosocomialis M2

40.268

100

0.438
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

98.54

0.431
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.727

100

0.423
  pilA Ralstonia pseudosolanacearum GMI1000

42.963

98.54

0.423
  pilA Vibrio parahaemolyticus RIMD 2210633

45.528

89.781

0.409
  pilE Neisseria gonorrhoeae MS11

35.065

100

0.394

Multiple sequence alignment