Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HSX14_RS06175 Genome accession   NZ_AP023189
Coordinates   1324246..1325949 (-) Length   567 a.a.
NCBI ID   WP_173173301.1    Uniprot ID   A0A6J4E108
Organism   Pseudomonas tohonis strain TUM18999     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1319246..1330949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX14_RS06135 (TUM18999_12180) - 1319445..1319870 (+) 426 WP_173173313.1 GNAT family N-acetyltransferase -
  HSX14_RS06140 (TUM18999_12190) - 1319867..1320334 (+) 468 WP_111263619.1 FAD/FMN-containing dehydrogenase -
  HSX14_RS06145 (TUM18999_12200) - 1320331..1321017 (+) 687 WP_173173311.1 energy-coupling factor ABC transporter permease -
  HSX14_RS06150 (TUM18999_12210) - 1321125..1321340 (+) 216 WP_173173309.1 hypothetical protein -
  HSX14_RS06155 (TUM18999_12220) yacG 1321344..1321544 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  HSX14_RS06160 (TUM18999_12230) coaE 1321541..1322152 (-) 612 WP_173173307.1 dephospho-CoA kinase -
  HSX14_RS06165 (TUM18999_12240) pilD 1322152..1323021 (-) 870 WP_173173305.1 prepilin peptidase Machinery gene
  HSX14_RS06170 (TUM18999_12250) pilC 1323022..1324242 (-) 1221 WP_173173303.1 type II secretion system F family protein Machinery gene
  HSX14_RS06175 (TUM18999_12260) pilB 1324246..1325949 (-) 1704 WP_173173301.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HSX14_RS06180 (TUM18999_12270) pilA 1326045..1326458 (-) 414 WP_173173299.1 pilin Machinery gene
  HSX14_RS06185 (TUM18999_12280) - 1326609..1328321 (+) 1713 WP_173173297.1 PglL family O-oligosaccharyltransferase -
  HSX14_RS06195 (TUM18999_12290) nadC 1328498..1329346 (-) 849 WP_173173295.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62536.67 Da        Isoelectric Point: 5.6312

>NTDB_id=81578 HSX14_RS06175 WP_173173301.1 1324246..1325949(-) (pilB) [Pseudomonas tohonis strain TUM18999]
MSDSIPLTGLARQIVIANLLDEKNAQQAQQQALRNKMSLVTYIVQNKLVKSRPLAEIAAEQFGVAFFDLGAIDKESQPKE
LISEKLIRQHRLLPLWRRGNKLFIGVSDPTNHQAVTDVQFSTGLTTEAILVEDDKLGEAIEKFFDSANSGMEDLGDVDLD
GVDIESVDEDRDDKGTGAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGVLHEVARPPIQLASRISAR
LKVMASLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCAACKKEVEIPRDTLLKEGFP
EERIGTFKLFGPVGCDSCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAAQMRKDGFNDLRTSGLLKAIHGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=81578 HSX14_RS06175 WP_173173301.1 1324246..1325949(-) (pilB) [Pseudomonas tohonis strain TUM18999]
ATGAGCGACTCAATTCCCCTCACCGGCCTGGCTCGCCAGATTGTGATTGCCAACCTGCTCGACGAGAAAAATGCCCAACA
GGCTCAGCAGCAGGCCCTGCGCAACAAGATGTCGCTGGTGACGTACATCGTTCAGAACAAGCTGGTGAAGAGTCGTCCGC
TCGCGGAAATCGCTGCTGAACAGTTCGGCGTGGCATTTTTTGATCTGGGAGCCATCGACAAGGAGTCCCAACCCAAGGAG
TTGATCAGCGAGAAGCTGATCCGCCAGCATCGCCTGCTCCCTCTATGGCGCAGAGGCAACAAGCTCTTTATTGGTGTCTC
GGACCCTACCAACCATCAAGCTGTAACCGATGTGCAGTTCAGTACCGGTCTCACCACCGAAGCCATCCTGGTGGAAGACG
ACAAGCTCGGCGAGGCCATTGAGAAATTCTTCGACTCCGCAAATAGTGGCATGGAGGACCTCGGCGATGTAGACCTCGAT
GGGGTGGACATCGAAAGTGTCGACGAGGACAGGGACGACAAGGGCACAGGTGCAGAAGCTGACGATGCGCCTGTTGTGCG
CTTCGTCAACAAGATGCTGCTGGACGCTATCAAAGGCGGTTCGTCAGACTTGCACTTCGAGCCTTATGAGAAGAGTTACC
GCGTCCGTCTCCGCACCGACGGCGTGCTGCATGAGGTCGCCCGCCCACCGATTCAACTGGCCAGCCGCATATCGGCACGC
CTCAAGGTAATGGCTTCCCTGGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGTATCAAAATGAAGATATCCAAAAC
CAAGGCCATCGATTTTCGCGTCAACACCTTGCCCACGCTGTGGGGAGAGAAGATTGTGATGCGAATCCTTGACCCCTCAA
GCGCGCAGATGGGCATCGATGCGCTGGGGTATGAGGAGTCGCAGAAAGAGCTTTATCTCAATGCCTTGAAACAGCCACAG
GGCATGATCCTGGTGACCGGCCCAACCGGGTCAGGCAAGACGGTGTCCCTCTACACCGGCTTGAATATCCTCAACACCGT
CGACATCAACATCTCGACAGCCGAAGACCCCGTTGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTGAACCCCCGGC
AAGGTATGGACTTTTCCCAGGCCCTTCGAGCCTTCCTCCGCCAGGACCCCGACGTCATCATGGTGGGTGAGATCCGCGAT
CTGGAGACAGCTGAGATCGCGATCAAGGCCGCTCAAACAGGACACATGGTGATGTCCACCCTTCACACCAACAGCGCCGC
TGAGACGCTGACACGGTTGCGTAACATGGGGGTGCCGGCGTTCAACATCGCCACATCGGTCAACCTGATCATTGCCCAAC
GACTTGCGCGAAAGCTTTGCGCCGCCTGCAAGAAGGAAGTAGAGATCCCACGCGATACGCTGCTCAAGGAAGGCTTCCCG
GAAGAGCGGATCGGAACCTTCAAGCTCTTTGGGCCCGTAGGTTGCGATAGCTGTAAAGGCGGCTACAAGGGAAGGGTGGG
TATTTATGAAGTGGTTAAAAACACTCCTGCCCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGATATCGCAGCTC
AGATGCGTAAAGATGGCTTCAACGATCTCCGCACTTCGGGGCTCTTGAAAGCCATCCATGGCGTGACGAGCCTCGAAGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6J4E108

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.043

99.471

0.587
  pilB Acinetobacter baylyi ADP1

58.082

99.295

0.577
  pilB Legionella pneumophila strain ERS1305867

55.379

100

0.554
  pilB Vibrio campbellii strain DS40M4

49.027

99.647

0.489
  pilB Vibrio cholerae strain A1552

49.464

98.765

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

50.093

95.062

0.476
  pilF Neisseria gonorrhoeae MS11

47.78

99.295

0.474
  pilF Thermus thermophilus HB27

36.252

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.606

93.651

0.362

Multiple sequence alignment