Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HSX14_RS06170 Genome accession   NZ_AP023189
Coordinates   1323022..1324242 (-) Length   406 a.a.
NCBI ID   WP_173173303.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain TUM18999     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1318022..1329242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX14_RS06125 (TUM18999_12160) - 1318446..1318619 (-) 174 WP_173173318.1 DUF3094 domain-containing protein -
  HSX14_RS06130 (TUM18999_12170) - 1318706..1319332 (-) 627 WP_173173316.1 DUF1780 domain-containing protein -
  HSX14_RS06135 (TUM18999_12180) - 1319445..1319870 (+) 426 WP_173173313.1 GNAT family N-acetyltransferase -
  HSX14_RS06140 (TUM18999_12190) - 1319867..1320334 (+) 468 WP_111263619.1 FAD/FMN-containing dehydrogenase -
  HSX14_RS06145 (TUM18999_12200) - 1320331..1321017 (+) 687 WP_173173311.1 energy-coupling factor ABC transporter permease -
  HSX14_RS06150 (TUM18999_12210) - 1321125..1321340 (+) 216 WP_173173309.1 hypothetical protein -
  HSX14_RS06155 (TUM18999_12220) yacG 1321344..1321544 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  HSX14_RS06160 (TUM18999_12230) coaE 1321541..1322152 (-) 612 WP_173173307.1 dephospho-CoA kinase -
  HSX14_RS06165 (TUM18999_12240) pilD 1322152..1323021 (-) 870 WP_173173305.1 prepilin peptidase Machinery gene
  HSX14_RS06170 (TUM18999_12250) pilC 1323022..1324242 (-) 1221 WP_173173303.1 type II secretion system F family protein Machinery gene
  HSX14_RS06175 (TUM18999_12260) pilB 1324246..1325949 (-) 1704 WP_173173301.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HSX14_RS06180 (TUM18999_12270) pilA 1326045..1326458 (-) 414 WP_173173299.1 pilin Machinery gene
  HSX14_RS06185 (TUM18999_12280) - 1326609..1328321 (+) 1713 WP_173173297.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44313.09 Da        Isoelectric Point: 9.9851

>NTDB_id=81577 HSX14_RS06170 WP_173173303.1 1323022..1324242(-) (pilC) [Pseudomonas tohonis strain TUM18999]
MAEKALKTTLFSWEGTDKKGARVKGDMSGTNPALVKAQLRKQGINPTKVRKKSASLFGKGKKIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGVENQNMRKLVDEVKQEVAAGNSFANSLRKKPLYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIKKAMTYPIAVILVALIVSAILLIKVVPQFQSVFSGFGAELPAFTMMVIHLSEFLQTWWFVVLLGMFGTAFGLR
ELHRRSKKFRDNVDRGVLKLPIVGDILYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNVVFKDAVTKIKQDVSTGM
QLNFSMRTTGVFPSMAIQMTAIGEESGALDTMLDKVASFYEEEVDNAVDNLTTLMEPLIMAVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=81577 HSX14_RS06170 WP_173173303.1 1323022..1324242(-) (pilC) [Pseudomonas tohonis strain TUM18999]
ATGGCGGAAAAAGCTCTCAAAACCACCCTGTTCAGCTGGGAAGGTACAGATAAAAAAGGCGCCAGGGTCAAAGGGGACAT
GAGCGGCACCAACCCCGCTCTTGTTAAAGCCCAGCTTCGTAAGCAGGGCATCAACCCCACCAAAGTCAGGAAAAAAAGCG
CATCCCTTTTTGGCAAGGGAAAGAAAATCAAGCCTCTAGATATAGCCCTGTTCACTCGCCAGATGGCTACCATGATGAAA
GCAGGCGTTCCCTTGCTTCAGAGCTTTGACATCATTGGAGAGGGCGTCGAAAACCAAAACATGCGAAAACTGGTTGACGA
GGTCAAACAGGAGGTAGCCGCAGGGAACAGCTTTGCAAACTCTCTAAGAAAAAAACCTCTGTATTTTGACGAACTCTACT
GCAACTTGGTGGACGCTGGCGAGCAGTCCGGCGCTTTGGAAAACCTGCTGGATCGAGTGGCTACCTATAAGGAGAAAACC
GAATCGCTCAAGGCAAAAATCAAGAAGGCAATGACCTACCCGATCGCCGTAATCTTGGTTGCCTTGATAGTTTCTGCAAT
CCTGCTTATTAAAGTTGTTCCGCAATTTCAGTCCGTTTTTTCAGGTTTCGGCGCTGAATTACCCGCTTTCACAATGATGG
TTATTCATCTTTCCGAATTTCTTCAGACATGGTGGTTCGTCGTGTTACTCGGCATGTTCGGAACCGCTTTCGGCCTCCGA
GAGCTTCATCGCCGCTCAAAAAAATTCAGAGATAACGTCGACCGAGGCGTGCTCAAGTTACCCATCGTCGGCGACATACT
CTACAAATCTGCCGTCGCACGTTACGCCCGTACGCTGTCCACCACCTTTGCAGCAGGTGTGCCTCTTGTAGACGCCCTCG
ACTCCGTTTCCGGCGCAACCGGTAACGTCGTATTCAAAGACGCGGTTACGAAGATCAAGCAGGATGTCTCTACCGGCATG
CAACTGAACTTCTCCATGCGCACCACTGGTGTATTTCCATCAATGGCCATTCAGATGACCGCCATCGGTGAAGAATCAGG
AGCCCTCGACACTATGCTCGACAAAGTCGCCTCCTTCTACGAAGAAGAAGTCGACAACGCCGTCGACAACCTGACCACCC
TAATGGAGCCCCTGATCATGGCGGTTCTCGGCGTGCTGGTCGGCGGCCTGATCATCGCGATGTACCTGCCTATCTTCCAA
CTCGGTTCGGTAGTGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

99.754

0.793
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Acinetobacter baumannii D1279779

59.259

99.754

0.591
  pilC Legionella pneumophila strain ERS1305867

55.303

97.537

0.539
  pilG Neisseria gonorrhoeae MS11

43.921

99.261

0.436
  pilG Neisseria meningitidis 44/76-A

43.564

99.507

0.433
  pilC Vibrio cholerae strain A1552

42.821

97.783

0.419
  pilC Vibrio campbellii strain DS40M4

42.317

97.783

0.414
  pilC Thermus thermophilus HB27

37.656

98.768

0.372

Multiple sequence alignment