Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QC826_RS24530 Genome accession   NZ_CP122407
Coordinates   5325107..5326150 (+) Length   347 a.a.
NCBI ID   WP_093388131.1    Uniprot ID   A0A1I4MYW3
Organism   Rugamonas sp. DEMB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5320107..5331150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC826_RS24505 (QC826_24505) ubiA 5321062..5321917 (+) 856 Protein_4852 4-hydroxybenzoate octaprenyltransferase -
  QC826_RS24510 (QC826_24510) - 5322011..5322376 (+) 366 WP_279762847.1 YqjD family protein -
  QC826_RS24515 (QC826_24515) - 5322508..5323302 (+) 795 WP_279762848.1 substrate-binding periplasmic protein -
  QC826_RS24520 (QC826_24520) proC 5323320..5324156 (-) 837 WP_279762849.1 pyrroline-5-carboxylate reductase -
  QC826_RS24525 (QC826_24525) - 5324185..5324988 (-) 804 WP_279762850.1 YggS family pyridoxal phosphate-dependent enzyme -
  QC826_RS24530 (QC826_24530) pilT 5325107..5326150 (+) 1044 WP_093388131.1 type IV pilus twitching motility protein PilT Machinery gene
  QC826_RS24535 (QC826_24535) pilU 5326159..5327292 (+) 1134 WP_279762851.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QC826_RS24540 (QC826_24540) - 5327292..5327777 (+) 486 WP_279762852.1 glutathione peroxidase -
  QC826_RS24545 (QC826_24545) - 5327947..5328552 (-) 606 WP_279762853.1 mobile mystery protein B -
  QC826_RS24550 (QC826_24550) - 5328531..5328977 (-) 447 WP_279762854.1 mobile mystery protein A -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38005.69 Da        Isoelectric Point: 6.7267

>NTDB_id=815481 QC826_RS24530 WP_093388131.1 5325107..5326150(+) (pilT) [Rugamonas sp. DEMB1]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKEVHGMIYDIMNDGQRKAYEEMLEVDFSFAIPGLAR
FRVNAYNQERGASAVLRTIPSKIMSLEELNCPRIFAELASKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPADEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMVATPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQT
LTDLVRRNVISAAAARSAAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=815481 QC826_RS24530 WP_093388131.1 5325107..5326150(+) (pilT) [Rugamonas sp. DEMB1]
ATGGACATCTCAGAACTTCTCGCATTCTCGGTCAAGAACAAGGCCTCGGACCTGCACTTGTCGGCCGGGCTGCCGCCGAT
GATACGGGTGCACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGGAAGTCCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCCTACGAGGAGATGCTCGAGGTCGACTTCTCCTTCGCCATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTACAACCAGGAACGCGGCGCCTCGGCGGTGCTGCGCACCATCCCGTCGAAGATCATGAGCCTGGA
GGAGTTGAACTGCCCGCGCATCTTCGCCGAGCTGGCCTCCAAGCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACCACGTCAACGAGAACGAGTACGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTCCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGGCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CCCTGTCGGCCGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGCATC
GTCGACGTCTTCCCGGCCGACGAGAAGGAGATGGTGCGGGCCATGCTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCAGCGGCCGCGTCGCCGCCCACGAGATCATGGTGGCCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGATCGCCCAGATGTACTCGGCGATCCAGACCGGCAGCAACGTCGGCATGCAGACGCTGGACCAGACG
CTGACCGACCTGGTGCGGCGCAACGTGATCTCGGCGGCGGCGGCCCGTTCCGCCGCCAAGATCCCGGACAACTTCCCCGG
ATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I4MYW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

73.623

99.424

0.732
  pilT Legionella pneumophila strain Lp02

74.631

97.695

0.729
  pilT Legionella pneumophila strain ERS1305867

74.631

97.695

0.729
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Neisseria gonorrhoeae MS11

70.294

97.983

0.689
  pilT Neisseria meningitidis 8013

70.294

97.983

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.118

0.671
  pilT Vibrio cholerae strain A1552

69.139

97.118

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

42.486

99.712

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401
  pilU Vibrio cholerae strain A1552

40.708

97.695

0.398