Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QC826_RS24535 Genome accession   NZ_CP122407
Coordinates   5326159..5327292 (+) Length   377 a.a.
NCBI ID   WP_279762851.1    Uniprot ID   -
Organism   Rugamonas sp. DEMB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5321159..5332292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC826_RS24510 (QC826_24510) - 5322011..5322376 (+) 366 WP_279762847.1 YqjD family protein -
  QC826_RS24515 (QC826_24515) - 5322508..5323302 (+) 795 WP_279762848.1 substrate-binding periplasmic protein -
  QC826_RS24520 (QC826_24520) proC 5323320..5324156 (-) 837 WP_279762849.1 pyrroline-5-carboxylate reductase -
  QC826_RS24525 (QC826_24525) - 5324185..5324988 (-) 804 WP_279762850.1 YggS family pyridoxal phosphate-dependent enzyme -
  QC826_RS24530 (QC826_24530) pilT 5325107..5326150 (+) 1044 WP_093388131.1 type IV pilus twitching motility protein PilT Machinery gene
  QC826_RS24535 (QC826_24535) pilU 5326159..5327292 (+) 1134 WP_279762851.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QC826_RS24540 (QC826_24540) - 5327292..5327777 (+) 486 WP_279762852.1 glutathione peroxidase -
  QC826_RS24545 (QC826_24545) - 5327947..5328552 (-) 606 WP_279762853.1 mobile mystery protein B -
  QC826_RS24550 (QC826_24550) - 5328531..5328977 (-) 447 WP_279762854.1 mobile mystery protein A -
  QC826_RS24555 (QC826_24555) - 5329318..5331831 (+) 2514 WP_279762855.1 putative bifunctional diguanylate cyclase/phosphodiesterase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41653.09 Da        Isoelectric Point: 6.5791

>NTDB_id=815482 QC826_RS24535 WP_279762851.1 5326159..5327292(+) (pilU) [Rugamonas sp. DEMB1]
MERDQAFKFMSDLLRLMVSKKGSDLFITAGFPPAIKIDGKLTPVSTQMLTAAHTVDLARAIMNDKQSAGFELSKEANFAI
SPGELGRFRVSAFVQMSSVGMVLRTITSEIPKLDDLGLPEVLKDVVMSKRGLVIMVGATGSGKSTTLAAMVDHRNQNSYG
HIITIEPVEYVHPHKNCIVTQREVGVDTEDWAVALKNTLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSAN
QALDRIINFFPEERRQQLLMDLSLNLKGMISQRLVPLKESAGRAVAIEILLNSPLISDLIFKGQVHEIKELMKKSRELGM
QTFDQSLFDLHEADKISYEDALRNADSVNDLRLAIKLEGKAAKNRDLSAGTEHLGII

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=815482 QC826_RS24535 WP_279762851.1 5326159..5327292(+) (pilU) [Rugamonas sp. DEMB1]
ATGGAACGCGACCAGGCATTCAAATTCATGTCCGACCTGCTGCGCCTGATGGTCAGCAAAAAAGGCTCGGACCTGTTCAT
CACCGCCGGCTTCCCGCCGGCCATCAAGATCGACGGCAAGCTGACGCCCGTCTCGACCCAGATGCTGACGGCGGCCCACA
CCGTCGACCTGGCGCGCGCTATCATGAACGACAAGCAGAGCGCCGGCTTCGAGCTGAGCAAGGAGGCCAACTTCGCCATC
AGCCCGGGCGAGCTGGGGCGCTTCCGCGTCTCCGCCTTCGTGCAGATGAGCTCGGTCGGCATGGTCTTGCGCACCATCAC
CAGCGAGATCCCGAAACTCGACGACCTGGGCCTGCCCGAGGTGCTCAAGGACGTCGTCATGTCCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACCGCAACCAGAACAGCTACGGC
CACATCATCACCATCGAACCGGTCGAGTACGTGCATCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTCGGCGTCGA
CACCGAGGACTGGGCGGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCGACC
GCGAGACCATGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCCAAC
CAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTCAACCTGAA
GGGCATGATCTCGCAGCGCCTGGTGCCGCTCAAGGAGAGCGCCGGGCGCGCCGTGGCGATCGAGATCCTGCTCAACTCGC
CGCTGATCTCCGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAGCTGATGAAGAAATCGCGCGAGCTGGGCATG
CAGACCTTCGACCAGTCGCTGTTCGATTTGCACGAAGCCGATAAAATCAGCTACGAGGACGCCTTGCGCAACGCCGATTC
GGTCAACGACCTGCGGCTGGCCATCAAACTCGAGGGCAAGGCGGCGAAAAACCGCGACCTCTCGGCCGGAACCGAACATT
TGGGGATCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(86-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.006

91.777

0.578
  pilU Acinetobacter baylyi ADP1

58.496

95.225

0.557
  pilU Vibrio cholerae strain A1552

57.185

90.451

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.73

89.39

0.382
  pilT Pseudomonas aeruginosa PAK

42.136

89.39

0.377
  pilT Legionella pneumophila strain ERS1305867

41.742

88.329

0.369
  pilT Legionella pneumophila strain Lp02

41.742

88.329

0.369
  pilT Acinetobacter nosocomialis M2

41.742

88.329

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.95

89.39

0.366
  pilT Acinetobacter baylyi ADP1

42.547

85.411

0.363