Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2042_RS14025 Genome accession   NZ_AP023187
Coordinates   2922695..2923735 (-) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio alginolyticus strain HLBS-07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2917695..2928735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2042_RS13995 (HLBS07_26950) endA 2917839..2918534 (+) 696 WP_053306786.1 deoxyribonuclease I -
  M2042_RS14000 (HLBS07_26960) rsmE 2918648..2919379 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M2042_RS14005 (HLBS07_26970) gshB 2919393..2920343 (+) 951 WP_005381021.1 glutathione synthase -
  M2042_RS14010 (HLBS07_26980) - 2920472..2921035 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  M2042_RS14015 (HLBS07_26990) ruvX 2921071..2921496 (+) 426 WP_104979707.1 Holliday junction resolvase RuvX -
  M2042_RS14020 (HLBS07_27000) pilU 2921558..2922664 (-) 1107 WP_064368776.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M2042_RS14025 (HLBS07_27010) pilT 2922695..2923735 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  M2042_RS14030 (HLBS07_27020) - 2923764..2924465 (+) 702 WP_104979709.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2042_RS14035 (HLBS07_27030) proC 2924625..2925443 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  M2042_RS14040 (HLBS07_27040) - 2925496..2926053 (+) 558 WP_005381012.1 YggT family protein -
  M2042_RS14045 (HLBS07_27050) yggU 2926053..2926343 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  M2042_RS14050 (HLBS07_27060) - 2926472..2926903 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  M2042_RS14055 (HLBS07_27070) - 2927022..2927624 (+) 603 WP_213891979.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=81548 M2042_RS14025 WP_005381015.1 2922695..2923735(-) (pilT) [Vibrio alginolyticus strain HLBS-07]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=81548 M2042_RS14025 WP_005381015.1 2922695..2923735(-) (pilT) [Vibrio alginolyticus strain HLBS-07]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGTATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCCTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCGGATGTGATTCTAGTCGGTGAGATGCGTGATAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCATACCAGCTCGGCGGCAAAAACTATTGACCGTATT
ATCGATGTTTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCTCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAATTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment