Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M2042_RS14020 Genome accession   NZ_AP023187
Coordinates   2921558..2922664 (-) Length   368 a.a.
NCBI ID   WP_064368776.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HLBS-07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2916558..2927664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2042_RS13990 (HLBS07_26930) - 2917191..2917688 (+) 498 WP_248525346.1 SprT family zinc-dependent metalloprotease -
  M2042_RS13995 (HLBS07_26950) endA 2917839..2918534 (+) 696 WP_053306786.1 deoxyribonuclease I -
  M2042_RS14000 (HLBS07_26960) rsmE 2918648..2919379 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M2042_RS14005 (HLBS07_26970) gshB 2919393..2920343 (+) 951 WP_005381021.1 glutathione synthase -
  M2042_RS14010 (HLBS07_26980) - 2920472..2921035 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  M2042_RS14015 (HLBS07_26990) ruvX 2921071..2921496 (+) 426 WP_104979707.1 Holliday junction resolvase RuvX -
  M2042_RS14020 (HLBS07_27000) pilU 2921558..2922664 (-) 1107 WP_064368776.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M2042_RS14025 (HLBS07_27010) pilT 2922695..2923735 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  M2042_RS14030 (HLBS07_27020) - 2923764..2924465 (+) 702 WP_104979709.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2042_RS14035 (HLBS07_27030) proC 2924625..2925443 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  M2042_RS14040 (HLBS07_27040) - 2925496..2926053 (+) 558 WP_005381012.1 YggT family protein -
  M2042_RS14045 (HLBS07_27050) yggU 2926053..2926343 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  M2042_RS14050 (HLBS07_27060) - 2926472..2926903 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  M2042_RS14055 (HLBS07_27070) - 2927022..2927624 (+) 603 WP_213891979.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41284.39 Da        Isoelectric Point: 6.5943

>NTDB_id=81547 M2042_RS14020 WP_064368776.1 2921558..2922664(-) (pilU) [Vibrio alginolyticus strain HLBS-07]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTSSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=81547 M2042_RS14020 WP_064368776.1 2921558..2922664(-) (pilU) [Vibrio alginolyticus strain HLBS-07]
ATGGATCTAGATAAATTTCTCGAAGGCATGTTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTTACCGAGAGCGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCAAATTTTGCCATTGTGAGAGATTGTGGCCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACCAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCAATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCATATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGAGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment