Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS04_RS17305 Genome accession   NZ_CP121854
Coordinates   3674216..3675250 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 13997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3669216..3680250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS04_RS17275 glnK 3669799..3670137 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS04_RS17280 arfB 3670339..3670755 (+) 417 WP_019445599.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS04_RS17285 - 3670787..3671278 (+) 492 WP_279465306.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS04_RS17290 - 3671637..3672062 (-) 426 WP_005340360.1 hypothetical protein -
  QBS04_RS17295 yaaA 3672257..3673030 (-) 774 WP_279465307.1 peroxide stress protein YaaA -
  QBS04_RS17300 pilU 3673078..3674187 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  QBS04_RS17305 pilT 3674216..3675250 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS04_RS17310 - 3675290..3675991 (+) 702 WP_139441948.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS04_RS17315 proC 3676134..3676958 (+) 825 WP_279465308.1 pyrroline-5-carboxylate reductase -
  QBS04_RS17320 - 3676970..3677521 (+) 552 WP_047436998.1 YggT family protein -
  QBS04_RS17325 yggU 3677521..3677823 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS04_RS17330 - 3677867..3678286 (+) 420 WP_033135536.1 DUF4426 domain-containing protein -
  QBS04_RS17335 - 3678388..3679584 (-) 1197 WP_113740087.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=814806 QBS04_RS17305 WP_005340354.1 3674216..3675250(-) (pilT) [Aeromonas veronii strain ANYA 13997]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814806 QBS04_RS17305 WP_005340354.1 3674216..3675250(-) (pilT) [Aeromonas veronii strain ANYA 13997]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAATGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGTGGTCTGGTGTTGGTGACCGGCCCGACCGGCT
CGGGTAAGTCGACCACTTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTACATCGAGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACTATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCAGGTGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGTCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGTATGCAGACCATGGATCAGAGTCTC
AAGCAGTTGGTGAGTCGTGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407