Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS07_RS07360 Genome accession   NZ_CP121837
Coordinates   1542490..1543524 (+) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18263     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537490..1548524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS07_RS07330 - 1538156..1539352 (+) 1197 WP_279488827.1 NupC/NupG family nucleoside CNT transporter -
  QBS07_RS07335 - 1539454..1539873 (-) 420 WP_113740088.1 DUF4426 domain-containing protein -
  QBS07_RS07340 yggU 1539917..1540219 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS07_RS07345 - 1540219..1540770 (-) 552 WP_005340343.1 YggT family protein -
  QBS07_RS07350 proC 1540782..1541606 (-) 825 WP_042053316.1 pyrroline-5-carboxylate reductase -
  QBS07_RS07355 - 1541749..1542450 (-) 702 WP_279488828.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS07_RS07360 pilT 1542490..1543524 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS07_RS07365 pilU 1543553..1544662 (+) 1110 WP_101530224.1 type IVa pilus ATPase TapU Machinery gene
  QBS07_RS07370 yaaA 1544710..1545483 (+) 774 WP_064338818.1 peroxide stress protein YaaA -
  QBS07_RS07375 - 1545678..1546103 (+) 426 WP_279488829.1 hypothetical protein -
  QBS07_RS07380 - 1546465..1546956 (-) 492 WP_279488830.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS07_RS07385 arfB 1546987..1547403 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS07_RS07390 glnK 1547605..1547943 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=814525 QBS07_RS07360 WP_005340354.1 1542490..1543524(+) (pilT) [Aeromonas veronii strain ANYA 18263]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814525 QBS07_RS07360 WP_005340354.1 1542490..1543524(+) (pilT) [Aeromonas veronii strain ANYA 18263]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTTATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTAGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTACCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCACGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGCT
CGGGTAAGTCGACCACTTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
TGCTCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACCGCCGCCGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCAGGCGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGAGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGTCTC
AAGCAGCTGGTGAGTCGCGGCGTAGTGGCATCCCTCGATGCTAAGGCCAAGGCCGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407